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AB241985

Anti-C5orf25 antibody

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(2 Publications)

Rabbit Polyclonal C5orf25 antibody. Suitable for IP and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SIMC1 aa 1-100.

View Alternative Names

C5orf25, PLEIAD, SIMC1, SUMO-interacting motif-containing protein 1, Platform element for inhibition of autolytic degradation

1 Images
Immunoprecipitation - Anti-C5orf25 antibody (AB241985)
  • IP

Supplier Data

Immunoprecipitation - Anti-C5orf25 antibody (AB241985)

C5orf25 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241985 at 8 μL per reaction. Western blot was performed from the immunoprecipitate using ab241985 at 1/1000 dilution.

Lane 1 : ab241985 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG in HEK-293T whole cell lysate.

Detection : Chemiluminescence with an exposure time of 30 seconds.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-C5orf25 antibody (ab241985)

Predicted band size: 97 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP

applications

Immunogen

Synthetic Peptide within Human SIMC1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q8NDZ2

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>8 - 15 μl/mg lysate</p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibody was affinity purified using an epitope specific to C5orf25 immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C5orf25 also known as Chromosome 5 open reading frame 25 is a protein with a molecular weight of approximately 17 kDa. This protein shows expression in various tissues with notable presence in the brain and heart. C5orf25 has no definitive enzymatic function identified yet but studies suggest it might play roles in cellular processes through potential interactions with other cellular components. Understanding the mechanical role of C5orf25 remains an active area of research.
Biological function summary

This protein contributes to intracellular functions although not clearly defined. C5orf25 may partake in cellular signaling or structural integrity within specific cells. It does not appear as part of any well-characterized protein complex currently suggesting that it might function independently or as part of transient interactions in cellular biology. This protein’s biological roles could influence how cells respond to stress or maintain their regular operations.

Pathways

Researchers have begun mapping its interactions to various cellular processes. Current findings suggest potential links to signal transduction pathways such as the MAPK signaling. In this context it might interact indirectly with proteins like ERK and MEK known for regulating cell growth and differentiation. Although these connections are still theoretical they form the basis for further investigations into how C5orf25 fits into broader cellular networks.

Researchers have explored links between C5orf25 and neurological conditions. Some studies propose that variations in this protein might associate with neurodevelopmental disorders alongside proteins like DISC1. Furthermore altered expression levels of C5orf25 in cardiac tissues have drawn attention to possible involvement in heart diseases. Understanding these associations could help develop biomarkers or novel therapeutic targets leveraging the relationship between C5orf25 and disease pathways.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Plays a role in SMC5-SMC6 complex recruitment for viral restriction. Forms a complex with SLF2 and this complex is required to recruit SMC5-SMC6 complex to PML nuclear bodies and sites of viral replication.. Isoform 1. Inhibits the protease activity of CAPN3.. Isoform 5. Inhibits the protease activity of CAPN3.
See full target information SIMC1

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

eLife 11: PubMed36373674

2022

The Nse5/6-like SIMC1-SLF2 complex localizes SMC5/6 to viral replication centers.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Oravcová,Minghua Nie,Nicola Zilio,Shintaro Maeda,Yasaman Jami-Alahmadi,Eros Lazzerini-Denchi,James A Wohlschlegel,Helle D Ulrich,Takanori Otomo,Michael N Boddy

Frontiers in cell and developmental biology 7:351 PubMed31921861

2019

Role of TGF-β/Smad Pathway in the Transcription of Pancreas-Specific Genes During Beta Cell Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhua Gao,Ranxi Zhang,Shanshan Dai,Xue Zhang,Xiangchen Li,Chunyu Bai
View all publications

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