Rabbit Polyclonal C5orf45 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MRNIP aa 50-200.
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- Nature communications 13:26382022MRNIP condensates promote DNA double-strand break sensing and end resection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun-Long Wang,Wan-Wen Zhao,Shao-Mei Bai,Li-Li Feng,Shu-Ying Bie,Li Gong,Fang Wang,Ming-Biao Wei,Wei-Xing Feng,Xiao-Lin Pang,Cao-Litao Qin,Xin-Ke Yin,Ying-Nai Wang,Weihua Zhou,Daniel R Wahl,Quentin Liu,Ming Chen,Mien-Chie Hung,Xiang-Bo WanPubMed 35551189
- Science advances 6:eaba59742020MRNIP is a replication fork protection factor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesL G Bennett et. al.PubMed 32832601
- Cell reports 16:2565-25752016MRNIP/C5orf45 Interacts with the MRN Complex and Contributes to the DNA Damage Response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristopher J Staples et. al.PubMed 27568553
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MRNIP aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q6NTE8
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100 |
Target data
Function
Plays a role in the cellular response to DNA damage and the maintenance of genome stability through its association with the MRN damage-sensing complex (PubMed:27568553). Promotes chromatin loading and activity of the MRN complex to facilitate subsequent ATM-mediated DNA damage response signaling and DNA repair (PubMed:27568553).
Alternative names
C5orf45, MRNIP, MRN complex-interacting protein, MRN-interacting protein
Recommended products
Rabbit Polyclonal C5orf45 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MRNIP aa 50-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MRNIP aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q6NTE8
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
C5orf45 also known as Chromosome 5 open reading frame 45 is a protein encoded by the C5orf45 gene. This protein has an approximate mass of 27 kDa. It is mainly expressed in the brain tissue and other areas at lower levels indicating a potential specialized function. Structurally C5orf45's exact mechanical function is not fully understood which suggests that more research may be required for the determination of its precise role in cellular mechanisms.
- Biological function summary
C5orf45 may contribute to cellular development processes in the brain. It has been suggested that C5orf45 interacts with other proteins to form functional complexes that may facilitate cell communication or growth regulation. These interactions make it valuable for proper cellular function especially in neural networks where timing and precision are important. The protein does not function in isolation but likely co-operates as part of larger protein networks.
- Pathways
Researchers suggest that C5orf45 might participate in signal transduction pathways related to neurodevelopment. It may interact with proteins in these pathways to influence how neuron signals travel and affect brain development. While specific interactions in these pathways are yet to be conclusively mapped the involvement of C5orf45 alongside other protein regulators like NRP1 (Neuropilin-1) points towards a role in neuronal signaling dynamics.
- Associated diseases and disorders
C5orf45's function potentially links it to neurodevelopmental disorders such as autism spectrum disorder (ASD) and schizophrenia. Some studies have shown changes in the expression levels of C5orf45 in individuals with these conditions. The relationship between C5orf45 and proteins like FMR1 (Fragile X Mental Retardation 1) suggests a complex network may influence the emergence and progression of these disorders. This indicates that C5orf45 may play a contributory role in neurological stability and could be a target for therapeutic research.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5orf45 antibody (ab150917)
Immunohistochemical analysis of human Fallopian tube tissue labeling C5orf45 in the nucleus of glandular cells with ab150917 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-C5orf45 antibody (ab150917)
Immunocytochemical analysis of A431 (Human epidermoid carcinoma cell line) whole cells labeling C5orf45 in the plasma membrane and cytoplasm with ab150917 at 2 μg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5orf45 antibody (ab150917)
Immunohistochemical analysis of human endometrium tissue labeling C5orf45 in the nucleus of glandular cells with ab150917 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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