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Rabbit Polyclonal C6 antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Complement component C6 aa 500-700.

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Images

Western blot - Anti-C6 antibody (AB229992), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C6 antibody (AB229992), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human Complement component C6 aa 500-700. The exact immunogen used to generate this antibody is proprietary information. Database link P13671

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Expected
Tested
Mouse
Tested
Expected
Rat
Tested
Expected
Chimpanzee
Predicted
Predicted

Tested
Tested

Species

Rat

Dilution info

1/500 - 1/5000

Notes

-

Species

Mouse

Dilution info

1/500 - 1/5000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Chimpanzee

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/20 - 1/200

Notes

-

Expected
Expected

Species

Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Chimpanzee

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells.

Alternative names

Recommended products

Rabbit Polyclonal C6 antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Complement component C6 aa 500-700.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human Complement component C6 aa 500-700. The exact immunogen used to generate this antibody is proprietary information. Database link P13671
Purification technique

Affinity purification Protein G

Concentration
Loading...
Purification notes

Purity >95%

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Complement component C6 often called simply C6 is an essential protein in the complement system. It is part of the terminal complement pathway which leads to the formation of the membrane attack complex (MAC). C6 has a molecular weight of about 93 kDa and is mainly produced in the liver. It circulates in plasma and interacts with other complement proteins to form MAC aiding in pathogen elimination and cell lysis.

Biological function summary

The role of C6 is the progression and stabilization of the MAC complex which is part of the innate immune system's defense. C6 collaborates with other complement proteins such as C5b C7 C8 and C9. This cooperation helps penetrate the membrane of pathogenic cells leading to cell destruction. Detecting and quantifying C6 levels is possible using techniques like complement C6 ELISA and C6 graphs valuable for research into immune response mechanisms.

Pathways

The complement component C6 is an important player in the terminal complement pathway and the larger complement activation pathway. Within these pathways C6 interacts closely with proteins like C5b and C9 to facilitate the assembly of the MAC. C6's involvement is critical for mounting an efficient immune response against invading pathogens making it an important focus for immunology studies involving the complement cascade.

Associated diseases and disorders

C6 deficiencies are linked to increased susceptibility to infections particularly by Neisseria species which cause diseases like meningitis. Individuals with C6-related complement deficiencies might show recurrent bacterial infections highlighting its role in immune defense. The protein's interplay with other complement components notably C5 and C7 can influence the disease states and inform potential therapeutic approaches aimed at boosting host defense mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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2 product images

  • Western blot - Anti-C6 antibody (ab229992), expandable thumbnail

    Western blot - Anti-C6 antibody (ab229992)

    All lanes: Western blot - Anti-C6 antibody (ab229992) at 1/500 dilution

    Lane 1: Rat liver tissue lysate

    Lane 2: Mouse kidney tissue lysate

    Secondary

    All lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 105 kDa

    Observed band size: 105 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C6 antibody (ab229992), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C6 antibody (ab229992)

    Paraffin-embedded human kidney tissue stained for C6 using ab229992 at 1/100 dilution in immunohistochemical analysis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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