AB251004

Anti-C6orf64 antibody [EPR15780] - BSA and Azide free

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal C6orf64 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C6orf64, SAYSD1, SAYSvFN domain-containing protein 1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C6orf64 antibody [EPR15780] - BSA and Azide free (AB251004)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C6orf64 antibody [EPR15780] - BSA and Azide free (AB251004)

This data was developed using ab189918, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling C6orf64 with ab189918 at 1/50 with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-C6orf64 antibody [EPR15780] - BSA and Azide free (AB251004)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-C6orf64 antibody [EPR15780] - BSA and Azide free (AB251004)

This data was developed using ab189918, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of paraformaldehyde-fixed HepG2 cells labeling C6orf64 with ab189918 at 1/100, Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and DAPI staining (blue).

Supplier Data

Immunoprecipitation - Anti-C6orf64 antibody [EPR15780] - BSA and Azide free (AB251004)

This data was developed using ab189918, the same antibody clone in a different buffer formulation.

Immunoprecipitation of C6orf64 from Human fetal liver lysate using ab189918 at 1/50 or 1X PBS (negative control) (lane 2). Immunostained with ab189918 at 1/10000 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-C6orf64 antibody [EPR15780] - C-terminal (<a href='/en-us/products/primary-antibodies/c6orf64-antibody-epr15780-c-terminal-ab189918'>ab189918</a>)

Predicted band size: 20 kDa

false

Supplier Data

Western blot - Anti-C6orf64 antibody [EPR15780] - BSA and Azide free (AB251004)

This data was developed using ab189918, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-C6orf64 antibody [EPR15780] - C-terminal (<a href='/en-us/products/primary-antibodies/c6orf64-antibody-epr15780-c-terminal-ab189918'>ab189918</a>) at 1/10000 dilution

Lane 1:

Human testis cell lysate at 20 µg

Lane 2:

Human fetal liver tissue lysate at 20 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa

Observed band size: 20 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15780

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

ab251004 is the carrier-free version of ab189918.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C6orf64 also known as FAM217B is a protein-coding gene with a molecular weight of around 23 kDa. This protein is present in multiple tissues with higher levels identified in the brain and testis. Researchers have not fully elucidated its mechanical function but studies suggest that it interacts with various cellular components influencing molecular signaling and cellular processes.

Biological function summary

C6orf64 has roles in cellular growth and proliferation. While it is not part of a known multi-protein complex it associates with other proteins involved in cell cycle regulation. The presence of C6orf64 in the brain indicates a specific function related to neural cell operation which points to its importance in maintaining normal cellular function in these tissues.

Pathways

C6orf64 interacts with key cellular signaling pathways including the PI3K-AKT pathway. It associates with proteins like AKT1 which play a role in cell survival and growth. This ties C6orf64 to important signaling processes that affect cellular decisions impacting activities like metabolism and cell cycle progression.

C6orf64 shows a relation to conditions such as cancer and neurodegenerative diseases. In cancer its interaction with AKT1 positions it as potentially influential in tumorigenic processes. In neurodegenerative diseases its significant presence in the brain and potential interaction with proteins involved in neural health suggest its involvement. Nevertheless further exploration is necessary to establish clearer connections.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Ufmylation 'reader' component of a translocation-associated quality control pathway, a mechanism that takes place when a ribosome has stalled during translation, and which is required to degrade clogged substrates (PubMed : 36848233). Specifically recognizes and binds ufmylated ribosomes when a ribosome has stalled, promoting the transport of stalled nascent chain via the TRAPP complex to lysosomes for degradation (PubMed : 36848233).

See full target information SAYSD1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Genes 14: PubMed37239471

2023

Differential Regulation of POC5 by ERα in Human Normal and Scoliotic Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Amani Hassan,Edward T Bagu,Shunmoogum A Patten,Sirinart Molidperee,Stefan Parent,Soraya Barchi,Isabelle Villemure,André Tremblay,Florina Moldovan

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-C6orf64 antibody [EPR15780] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C6orf64 antibody [EPR15780] - BSA and Azide free (AB251004)

Immunocytochemistry/ Immunofluorescence - Anti-C6orf64 antibody [EPR15780] - BSA and Azide free (AB251004)

Immunoprecipitation - Anti-C6orf64 antibody [EPR15780] - BSA and Azide free (AB251004)

Western blot - Anti-C6orf64 antibody [EPR15780] - BSA and Azide free (AB251004)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise