AB189918

Anti-C6orf64 antibody [EPR15780] - C-terminal

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal C6orf64 antibody. C-terminal. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

C6orf64, SAYSD1, SAYSvFN domain-containing protein 1

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-C6orf64 antibody [EPR15780] - C-terminal (AB189918)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-C6orf64 antibody [EPR15780] - C-terminal (AB189918)

Immunofluorescent analysis of paraformaldehyde-fixed HepG2 cells labeling C6orf64 with ab189918 at 1/100 Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and DAPI staining (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C6orf64 antibody [EPR15780] - C-terminal (AB189918)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C6orf64 antibody [EPR15780] - C-terminal (AB189918)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling C6orf64 with ab189918 at 1/50 with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-C6orf64 antibody [EPR15780] - C-terminal (AB189918)

Immunoprecipitation of C6orf64 from Human fetal liver lysate using ab189918 at 1/50 or 1X PBS (negative control) (lane 2). Immunostained with ab189918 at 1/10000 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-C6orf64 antibody [EPR15780] - C-terminal (ab189918)

Predicted band size: 20 kDa

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Supplier Data

Western blot - Anti-C6orf64 antibody [EPR15780] - C-terminal (AB189918)

All lanes:

Western blot - Anti-C6orf64 antibody [EPR15780] - C-terminal (ab189918) at 1/10000 dilution

Lane 1:

Human testis cell lysate at 20 µg

Lane 2:

Human fetal liver tissue lysate at 20 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa

Observed band size: 20 kDa

false

Carrier free

Anti-C6orf64 antibody [EPR15780] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15780

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C6orf64 also known as FAM217B is a protein-coding gene with a molecular weight of around 23 kDa. This protein is present in multiple tissues with higher levels identified in the brain and testis. Researchers have not fully elucidated its mechanical function but studies suggest that it interacts with various cellular components influencing molecular signaling and cellular processes.

Biological function summary

C6orf64 has roles in cellular growth and proliferation. While it is not part of a known multi-protein complex it associates with other proteins involved in cell cycle regulation. The presence of C6orf64 in the brain indicates a specific function related to neural cell operation which points to its importance in maintaining normal cellular function in these tissues.

Pathways

C6orf64 interacts with key cellular signaling pathways including the PI3K-AKT pathway. It associates with proteins like AKT1 which play a role in cell survival and growth. This ties C6orf64 to important signaling processes that affect cellular decisions impacting activities like metabolism and cell cycle progression.

C6orf64 shows a relation to conditions such as cancer and neurodegenerative diseases. In cancer its interaction with AKT1 positions it as potentially influential in tumorigenic processes. In neurodegenerative diseases its significant presence in the brain and potential interaction with proteins involved in neural health suggest its involvement. Nevertheless further exploration is necessary to establish clearer connections.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ufmylation 'reader' component of a translocation-associated quality control pathway, a mechanism that takes place when a ribosome has stalled during translation, and which is required to degrade clogged substrates (PubMed : 36848233). Specifically recognizes and binds ufmylated ribosomes when a ribosome has stalled, promoting the transport of stalled nascent chain via the TRAPP complex to lysosomes for degradation (PubMed : 36848233).

See full target information SAYSD1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 42:112028 PubMed36848233

2023

SAYSD1 senses UFMylated ribosome to safeguard co-translational protein translocation at the endoplasmic reticulum.

Applications

Unspecified application

Species

Unspecified reactive species

Lihui Wang,Yue Xu,Sijung Yun,Quan Yuan,Prasanna Satpute-Krishnan,Yihong Ye

Cell death and differentiation 23:1765-1777 PubMed27391797

2016

Impaired DNA double-strand break repair contributes to the age-associated rise of genomic instability in humans.

Applications

Unspecified application

Species

Unspecified reactive species

Z Li,W Zhang,Y Chen,W Guo,J Zhang,H Tang,Z Xu,H Zhang,Y Tao,F Wang,Y Jiang,F L Sun,Z Mao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-C6orf64 antibody [EPR15780] - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-C6orf64 antibody [EPR15780] - C-terminal (AB189918)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C6orf64 antibody [EPR15780] - C-terminal (AB189918)

Immunoprecipitation - Anti-C6orf64 antibody [EPR15780] - C-terminal (AB189918)

Western blot - Anti-C6orf64 antibody [EPR15780] - C-terminal (AB189918)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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