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Rabbit Recombinant Monoclonal C6orf64 antibody. C-terminal. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 1 publication.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C6orf64 antibody [EPR15780] - C-terminal (AB189918), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-C6orf64 antibody [EPR15780] - C-terminal (AB189918), expandable thumbnail
  • Immunoprecipitation - Anti-C6orf64 antibody [EPR15780] - C-terminal (AB189918), expandable thumbnail
  • Western blot - Anti-C6orf64 antibody [EPR15780] - C-terminal (AB189918), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFIHC-P
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/10000 - 1/50000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Ufmylation 'reader' component of a translocation-associated quality control pathway, a mechanism that takes place when a ribosome has stalled during translation, and which is required to degrade clogged substrates (PubMed:36848233). Specifically recognizes and binds ufmylated ribosomes when a ribosome has stalled, promoting the transport of stalled nascent chain via the TRAPP complex to lysosomes for degradation (PubMed:36848233).

Alternative names

C6orf64, SAYSD1, SAYSvFN domain-containing protein 1

Recommended products

Rabbit Recombinant Monoclonal C6orf64 antibody. C-terminal. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR15780
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

C6orf64 also known as FAM217B is a protein-coding gene with a molecular weight of around 23 kDa. This protein is present in multiple tissues with higher levels identified in the brain and testis. Researchers have not fully elucidated its mechanical function but studies suggest that it interacts with various cellular components influencing molecular signaling and cellular processes.

Biological function summary

C6orf64 has roles in cellular growth and proliferation. While it is not part of a known multi-protein complex it associates with other proteins involved in cell cycle regulation. The presence of C6orf64 in the brain indicates a specific function related to neural cell operation which points to its importance in maintaining normal cellular function in these tissues.

Pathways

C6orf64 interacts with key cellular signaling pathways including the PI3K-AKT pathway. It associates with proteins like AKT1 which play a role in cell survival and growth. This ties C6orf64 to important signaling processes that affect cellular decisions impacting activities like metabolism and cell cycle progression.

Associated diseases and disorders

C6orf64 shows a relation to conditions such as cancer and neurodegenerative diseases. In cancer its interaction with AKT1 positions it as potentially influential in tumorigenic processes. In neurodegenerative diseases its significant presence in the brain and potential interaction with proteins involved in neural health suggest its involvement. Nevertheless further exploration is necessary to establish clearer connections.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C6orf64 antibody [EPR15780] - C-terminal (ab189918), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C6orf64 antibody [EPR15780] - C-terminal (ab189918)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling C6orf64 with ab189918 at 1/50 with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-C6orf64 antibody [EPR15780] - C-terminal (ab189918), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-C6orf64 antibody [EPR15780] - C-terminal (ab189918)

    Immunofluorescent analysis of paraformaldehyde-fixed HepG2 cells labeling C6orf64 with ab189918 at 1/100, Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and DAPI staining (blue).

  • Immunoprecipitation - Anti-C6orf64 antibody [EPR15780] - C-terminal (ab189918), expandable thumbnail

    Immunoprecipitation - Anti-C6orf64 antibody [EPR15780] - C-terminal (ab189918)

    Immunoprecipitation of C6orf64 from Human fetal liver lysate using ab189918 at 1/50 or 1X PBS (negative control) (lane 2). Immunostained with ab189918 at 1/10000 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    All lanes: Immunoprecipitation - Anti-C6orf64 antibody [EPR15780] - C-terminal (ab189918)

    Predicted band size: 20 kDa

  • Western blot - Anti-C6orf64 antibody [EPR15780] - C-terminal (ab189918), expandable thumbnail

    Western blot - Anti-C6orf64 antibody [EPR15780] - C-terminal (ab189918)

    All lanes: Western blot - Anti-C6orf64 antibody [EPR15780] - C-terminal (ab189918) at 1/10000 dilution

    Lane 1: Human testis cell lysate at 20 µg

    Lane 2: Human fetal liver tissue lysate at 20 µg

    Lane 3: 293T (Human embryonic kidney epithelial cell) cell lysate at 20 µg

    Lane 4: MCF7 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 20 kDa

    Observed band size: 20 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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