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AB288720

Anti-C7 antibody [EPR25751-34] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal C7 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

Complement component C7, C7

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Sandwich ELISA - Anti-C7 antibody [EPR25751-34] - BSA and Azide free (Capture) (AB288720)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-C7 antibody [EPR25751-34] - BSA and Azide free (Capture) (AB288720)

Sandwich ELISA of ab288736 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25751-34

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab288720 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human C7. The recommended pair for sandwich ELISA is:
Capture: ab288720, Human anti-C7 Capture Antibody (unconjugated)
Detector: ab288721, Human anti-C7 Detector Antibody (unconjugated)
The reference range value is 9.8-2500 pg/mL.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Complement component 7 (C7) with a molecular mass of approximately 93 kDa plays an essential mechanical role in the complement system a critical part of innate immunity. C7 is one of the late components of the complement cascade involved in the formation of the membrane attack complex (MAC). It is primarily expressed in the liver and found in the blood plasma. By associating with other components C5b and C6 C7 anchors into the lipid bilayer of target cells serving as a foothold for further MAC assembly.
Biological function summary

C7 functions as an important facilitator in the innate immune response being an integral part of the membrane attack complex (MAC). This complex forms transmembrane channels in the cell membrane of pathogens leading to their lysis and destruction. Through its interaction with complement components like C5b and C6 C7 enables the insertion and stabilization of the MAC. This process is important for the immune system’s ability to eliminate pathogens quickly and effectively protecting the host from microbial invaders.

Pathways

The complement system pathway is the primary biological pathway involving C7. Within this pathway C7 interacts closely with complement proteins C5 and C6 contributing to the terminal pathway that leads to pathogen lysis. Besides it is linked with the classical and alternative complement pathways both of which converge at the activation of C5. Through these interactions and processes C7 works synergistically with other complement proteins to execute robust immune defense mechanisms.

C7 deficiencies have been associated with an increased susceptibility to infections particularly by Neisseria species due to impaired MAC formation. This link highlights the importance of C7 for adequate host defense. Additionally overactivation of the complement system potentially involving C7 contributes to the pathology of autoimmune disorders like systemic lupus erythematosus. In such conditions dysregulation involving proteins like C5b and C6 can lead to tissue damage and inflammation further illustrating the importance of C7 in maintaining immune homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor.
See full target information C7
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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