AB185948

Anti-C7orf42 antibody [EPR15381]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal C7orf42 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

C7orf42, TMEM248, Transmembrane protein 248

3 Images

Flow Cytometry (Intracellular) - Anti-C7orf42 antibody [EPR15381] (AB185948)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-C7orf42 antibody [EPR15381] (AB185948)

Intracellular Flow Cytometry analysis of HeLa cells usingab185948 at 1/210 dilution. Secondary ab : Goat anti rabbit IgG (FITC) at 1/150 dilution. Fixative : 2% paraformaldehyde. Isotype control : Rabbit monoclonal IgG.

Supplier Data

Western blot - Anti-C7orf42 antibody [EPR15381] (AB185948)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-C7orf42 antibody [EPR15381] (ab185948) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

Raw264.7 cell lysate at 10 µg

Lane 3:

PC-12 cell lysate at 10 µg

Lane 4:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 35 kDa

false

Supplier Data

Western blot - Anti-C7orf42 antibody [EPR15381] (AB185948)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-C7orf42 antibody [EPR15381] (ab185948) at 1/10000 dilution

Lane 1:

Hela cell lysate at 20 µg

Lane 2:

MCF-7 cell lysate at 20 µg

Lane 3:

A549 cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15381

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/210", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C7orf42 also known by some researchers as FAM157A is a protein that has a molecular mass of around 33 kDa. It is expressed in several tissues with noticeable levels in the brain testis and skeletal muscle. This expression pattern suggests a role in specific physiological processes. Currently the detailed mechanical function of C7orf42 remains under study but researchers are actively investigating its role to understand its functions.

Biological function summary

C7orf42 may contribute to cellular homeostasis and signaling dynamics. It does not appear to be a part of any major complex but its independent role in the cellular environment warrants further study. Studies suggest C7orf42 could influence functions such as cellular proliferation and differentiation given its expression in rapidly changing environments like the testis and brain indicative of its participation in dynamic cellular processes.

Pathways

C7orf42 interacts with key signaling cascades. It has associations with the Wnt signaling pathway which is important for cell growth and differentiation. C7orf42 is related to proteins like β-catenin in the Wnt pathway suggesting its possible impact on development and cellular communication. It may also have a role in the PI3K-Akt signaling pathway which strongly influences cellular survival and growth hinting at a broader involvement in regulatory networks.

Current research links C7orf42 to certain neurological disorders and metabolic issues. Studies have found correlations with neural development disorders which points to its importance in brain physiology. Its connection with proteins in metabolic pathways also suggests possible implications in metabolic syndrome. Researchers are particularly interested in how it relates to proteins like mTOR a known regulator of cellular metabolism and growth suggesting areas for future investigation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information TMEM248

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 10:eadm8196 PubMed38787953

2024

Human DNA topoisomerase I poisoning causes R loop-mediated genome instability attenuated by transcription factor IIS.

Applications

Unspecified application

Species

Unspecified reactive species

Renée C Duardo,Jessica Marinello,Marco Russo,Sara Morelli,Simona Pepe,Federico Guerra,Belén Gómez-González,Andrés Aguilera,Giovanni Capranico

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-C7orf42 antibody [EPR15381]

Flow Cytometry (Intracellular) - Anti-C7orf42 antibody [EPR15381] (AB185948)

Western blot - Anti-C7orf42 antibody [EPR15381] (AB185948)

Western blot - Anti-C7orf42 antibody [EPR15381] (AB185948)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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