AB59140

Anti-C8 antibody [056B-373]

Name: Anti-C8 antibody [056B-373] (ab59140) | Abcam
Brand: Abcam Limited
SKU: AB59140
Price: 495 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal CO8A antibody. Suitable for Flow Cyt, ELISA, WB, IHC-Fr and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Native Full Length Protein corresponding to Human C8G.

View Alternative Names

Complement component C8 alpha chain, Complement component 8 subunit alpha, C8A, Complement component C8 beta chain, Complement component 8 subunit beta, C8B, Complement component C8 gamma chain, C8G

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

056B-373

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ELISA, IHC-Fr, Flow Cyt, WB

applications

Immunogen

Native Full Length Protein corresponding to Human C8G.

P07360

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.1% Sodium azide Constituents: 99% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C8 also known as complement component C8 is a protein that plays an important role in the immune system's complement pathway. This protein has a mass of approximately 151 kDa and is expressed mainly in the liver. It circulates in the blood plasma until activated. C8 is an integral part of the membrane attack complex (MAC) which forms pores in the cell membranes of pathogens leading to their destruction. The protein occurs as a heterotrimer composed of three subunits: alpha (C8A) beta (C8B) and gamma (C8G) each contributing to its function.

Biological function summary

Complement component C8 participates in the terminal phase of the complement cascade. Within this process it integrates into the MAC a large protein complex that forms on the surface of pathogens. The formation of MAC creates transmembrane channels that lead to cell lysis. C8 is part of a larger group of proteins known as the complement system which works to clear pathogens from an organism. Its assembly and function are dependent on previous steps in the complement activation pathway particularly interaction with components like C9 for forming the complete MAC.

Pathways

The complement component C8 is actively involved in the complement activation classical pathway and the alternative pathway. These pathways are important for the immune response aiding in the opsonization of pathogens and promoting inflammation. In the context of these pathways C8 interacts with other complement proteins such as C5b which is necessary for the initiation of MAC assembly. This interaction ensures the proper functioning of the complement responses safeguarding against infections.

Mutations or deficiencies in complement component C8 can lead to immunological abnormalities such as recurrent bacterial infections notably Neisseria infections. Individuals with C8 deficiency may suffer from increased susceptibility to such infections due to impaired MAC formation. Furthermore an inappropriate activation or regulation of complement pathways involving C8 can contribute to autoimmune diseases like systemic lupus erythematosus where other complement proteins like C3 also play an important role. Understanding the functioning of C8 in these contexts is essential for developing therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C8A inserts into the target membrane, but does not form pores by itself.

See full target information C8A

Additional targets

C8G,C8B

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Food & nutrition research 61:1290740 PubMed28326005

2017

Bioactivity of food peptides: biological response of rats to bovine milk whey peptides following acute exercise.

Applications

Unspecified application

Species

Unspecified reactive species

Carolina Soares Moura,Pablo Christiano Barboza Lollo,Priscila Neder Morato,Eder Muller Risso,Jaime Amaya-Farfan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-C8 antibody [056B-373]

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

Product promise