AB181182

Anti-C8G antibody [EPR11274] - C-terminal

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal CO8G antibody. C-terminal. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Complement component C8 gamma chain, C8G

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-C8G antibody [EPR11274] - C-terminal (AB181182)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-C8G antibody [EPR11274] - C-terminal (AB181182)

Immunofluorescence analysis of Human SW480 cells staining C8G (red) with ab181182 at 1/100 dilution. An Alexa Fluor^® 555-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

Supplier Data

Western blot - Anti-C8G antibody [EPR11274] - C-terminal (AB181182)

All lanes:

Western blot - Anti-C8G antibody [EPR11274] - C-terminal (ab181182) at 1/1000 dilution

Lane 1:

Human colon cancer lysate at 20 µg

Lane 2:

Human plasma at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa

false

Carrier free

Anti-C8G antibody [EPR11274] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11274

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C8G also known as complement component 8 gamma plays a significant role in the immune system's function. This protein has a mass of approximately 22 kDa and forms a part of the complement component 8 complex which acts as a critical player in the formation of the Membrane Attack Complex (MAC). Researchers have found C8G in various tissues with notable expression in the liver. The protein is generally released into the bloodstream as part of its involvement in immune responses.

Biological function summary

Complement component 8 gamma participates in puncturing pathogen cell membranes. It functions within the Membrane Attack Complex a structure important for antimicrobial activity. C8G partners with complement components C8 alpha and C8 beta to form the complete C8 complex which then associates with other complement proteins to facilitate membrane penetration. This activity highlights its role in protecting the host from infectious agents by disrupting pathogen integrity.

Pathways

C8G contributes critically to the complement cascade particularly within the terminal pathway. This pathway involves other complement proteins like C5b C6 and C7 coordinating to assemble the MAC which serves as a defense mechanism against microbial invasion. Consistently C8G shares functional relationships with these proteins reinforcing its participation in immune mediators.

C8G dysfunction associates with conditions like Complement Component 8 Deficiency which results in increased susceptibility to Neisseria infections. Moreover alterations in C8G can link to systemic lupus erythematosus an autoimmune disease where complement activation may become dysregulated. C8G's interactions with C8 alpha and C8 beta components remain important in these pathophysiological contexts potentially serving as targets for therapeutic interventions aimed at modulating complement activity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

C8 is a constituent of the membrane attack complex. C8 binds to the C5B-7 complex, forming the C5B-8 complex. C5-B8 binds C9 and acts as a catalyst in the polymerization of C9. The gamma subunit seems to be able to bind retinol.

See full target information C8G

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:106417 PubMed37153444

2023

Ultra-rare complement factor 8 coding variants in families with age-related macular degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Lina Zelinger,Tammy M Martin,Jayshree Advani,Laura Campello,Milton A English,Alan Kwong,Claire Weber,Jennifer Maykoski,Yuri V Sergeev,Robert Fariss,Emily Y Chew,Michael L Klein,Anand Swaroop

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-C8G antibody [EPR11274] - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-C8G antibody [EPR11274] - C-terminal (AB181182)

Western blot - Anti-C8G antibody [EPR11274] - C-terminal (AB181182)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise