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AB250296

Anti-C8orf30A antibody [EPR13203-28] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal C8orf30A antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C8orf30A, C8orf30B, FAM203A, FAM203B, HGH1, Co-chaperone protein HGH1 homolog

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C8orf30A antibody [EPR13203-28] - BSA and Azide free (AB250296)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C8orf30A antibody [EPR13203-28] - BSA and Azide free (AB250296)

This data was developed using ab181031, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling C8orf30A with ab181031 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C8orf30A antibody [EPR13203-28] - BSA and Azide free (AB250296)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C8orf30A antibody [EPR13203-28] - BSA and Azide free (AB250296)

This data was developed using ab181031, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human endometrium tissue labeling C8orf30A with ab181031 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-C8orf30A antibody [EPR13203-28] - BSA and Azide free (AB250296)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-C8orf30A antibody [EPR13203-28] - BSA and Azide free (AB250296)

This data was developed using ab181031, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HeLa cells (-20°C acetone-fixed) labeling C8orf30A with ab181031 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Immunoprecipitation - Anti-C8orf30A antibody [EPR13203-28] - BSA and Azide free (AB250296)
  • IP

Supplier Data

Immunoprecipitation - Anti-C8orf30A antibody [EPR13203-28] - BSA and Azide free (AB250296)

This data was developed using ab181031, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from HeLa cell lysate (+) or a negative control (-) immunoprecipitated using ab181031 at 1/40 dilution.

Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-C8orf30A antibody [EPR13203-28] (<a href='/en-us/products/primary-antibodies/c8orf30a-antibody-epr13203-28-ab181031'>ab181031</a>)

Predicted band size: 42 kDa

false

Western blot - Anti-C8orf30A antibody [EPR13203-28] - BSA and Azide free (AB250296)
  • WB

Supplier Data

Western blot - Anti-C8orf30A antibody [EPR13203-28] - BSA and Azide free (AB250296)

This data was developed using ab181031, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-C8orf30A antibody [EPR13203-28] (<a href='/en-us/products/primary-antibodies/c8orf30a-antibody-epr13203-28-ab181031'>ab181031</a>) at 1/5000 dilution

Lane 1:

Human fetal liver tissue lysate at 20 µg

Lane 2:

Human spleen tissue lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

Caco-2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 42 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13203-28

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250296 is the carrier-free version of ab181031.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The C8orf30A protein also called Chromosome 8 open reading frame 30A has a molecular mass of about 12 kDa. This protein's function involves cellular processes related to energy metabolism and plays an unclear yet significant mechanical role in the cells. Expression of C8orf30A occurs in tissues that display high metabolic activity such as liver and muscle tissues. Current research suggests its activity might impact the efficiency of cellular energy utilization.
Biological function summary

C8orf30A contributes to the regulation of mitochondrial function and energy metabolism. It forms a part of a larger protein complex that participates in the cellular energy management. The presence of this protein influences the mitochondrial respiratory chain affecting the production of ATP—a critical energy currency of the cell. The modulation of energy dynamics by C8orf30A highlights its importance in maintaining cellular energy homeostasis.

Pathways

C8orf30A involvement reflects in the regulation of oxidative phosphorylation and glycolytic pathways. This protein interacts closely with enzymes and other proteins within these pathways to modulate energy production and balance. It associates with proteins like ATP synthase and complexes involved in electron transport reflecting an essential integrative role within metabolic pathways to harness energy efficiently.

Changes in C8orf30A levels connect to metabolic disorders including diabetes and metabolic syndrome. The protein's regulation influences factors related to these diseases impacting insulin sensitivity and energy utilization in cells. C8orf30A interacts with proteins such as glucose transporters and alters their function emphasizing its role in disease progression and providing potential targets for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Co-chaperone involved in the folding of nascent eukaryotic elongation factor eEF2, an essential component of the translation machinery. Recognizes a non-native conformation in the central domain of eEF2 and recruits the chaperonin-containing T-complex (TRiC) to the C-terminal region of the nascent polypeptide, preventing unproductive interactions and promoting efficient folding of the N-terminal GTPase domain. May also assist in eEF2 maturation by recruiting the molecular chaperone Hsp90.
See full target information HGH1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com