AB17931

Anti-C9 antibody [53]

Name: Anti-C9 antibody [53] (ab17931) | Abcam
Brand: Abcam Limited
SKU: AB17931
Price: 495 USD
Availability: InStock

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(4 Publications)

Mouse Monoclonal C9 antibody. Suitable for Flow Cyt, ELISA, WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human C9.

View Alternative Names

Complement component C9, C9

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9 antibody [53] (AB17931)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9 antibody [53] (AB17931)

ab17931 staining human normal liver. Staining is localized to the cytoplasm.
Left panel : with primary antibody at 2 ug/ml. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer, EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Flow Cytometry - Anti-C9 antibody [53] (AB17931)

Flow Cyt

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Flow Cytometry - Anti-C9 antibody [53] (AB17931)

Overlay histogram showing HeLa cells stained with ab17931 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab17931, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

WB, ELISA, Flow Cyt, IHC-P

applications

Immunogen

Native Full Length Protein corresponding to Human C9.

P02748

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2 µg/mL", "IHCP-species-notes": "" } } }

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Product details

ab17931 recognizes C9 in human serum diluted 1:50 in Tris buffer (20 mM Tris-base, 1 mM MgCl2, 1 mM CaCl2 and 140 mM NaCl) and incubated for 2 hours at 37°C using a human IgM coated (10 μg/mL overnight at 4°C, blocked with PBS 7.2 + 1% BSA for 1 hour).

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Protein A/G purified.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.754% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C9 also known as complement component 9 is a protein that plays an essential mechanical role in the complement system. It typically associates in the formation of the membrane attack complex (MAC). The C9 protein weighing around 71 kDa expresses in the liver and is secreted into the blood plasma. Alternative names like 'Cloud 9 capture' and 'C9 tag' often refer to research tools or motifs derived from the C9 protein rather than the protein itself. Researchers frequently study C9 in mouse models where its physiological roles can be explored.

Biological function summary

C9 participates in defending against pathogens. It joins other complement components like C5b C6 C7 and C8 to form the MAC complex which facilitates cell lysis by forming pores in the membrane of target cells. This action directly results in microbial cell destruction serving a critical immune function. C9 is not only part of the MAC complex but also holds significance due to its unique function as the last step in the formation of the pore influencing the efficacy of pathogen elimination.

Pathways

C9 functions primarily within the complement cascade which includes the classical and alternative pathways. Its role is in the terminal pathway segment that ultimately leads to the formation of the MAC and follows the activation of C5 which cleaves to produce C5b. This connection extends to proteins like C3 which is upstream in the cascade and significantly impacts the cascade's activation highlighting C9's function as an integral terminal component in these pathways.

Mutations or deficits in C9 relate to an increased susceptibility to infections particularly those caused by Neisseria species. This association results directly from its important role in the MAC formation. Additionally deficiency of C9 links to autoimmune disorders where the immune response may be improperly regulated due to compromised MAC formation. Other complement components like C6 and C8 may also relate to these diseases due to their collaboration with C9 in forming the MAC illustrating the interconnectedness of these proteins within the immune response framework.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Pore-forming component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis (PubMed : 22832194, PubMed : 26841837, PubMed : 26841934, PubMed : 27052168, PubMed : 30552328, PubMed : 6177822, PubMed : 9212048, PubMed : 9634479). The MAC is initiated by proteolytic cleavage of C5 into complement C5b in response to the classical, alternative, lectin and GZMK complement pathways (PubMed : 39914456, PubMed : 39814882, PubMed : 9212048, PubMed : 9634479). The complement pathways consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 9212048, PubMed : 9634479). Constitutes the pore-forming subunit of the MAC complex : during MAC assembly, C9 associates with the C5b8 intermediate complex, and polymerizes to complete the pore (PubMed : 26841934, PubMed : 30111885, PubMed : 30552328, PubMed : 34752492, PubMed : 4055801, PubMed : 6177822).

See full target information C9

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cancers 13: PubMed34201241

2021

Development of EndoScreen Chip, a Microfluidic Pre-Endoscopy Triage Test for Esophageal Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Julie A Webster,Alain Wuethrich,Karthik B Shanmugasundaram,Renee S Richards,Wioleta M Zelek,Alok K Shah,Louisa G Gordon,Bradley J Kendall,Gunter Hartel,B Paul Morgan,Matt Trau,Michelle M Hill

Journal of cellular and molecular medicine 23:7449-7461 PubMed31512366

2019

Silencing of NONO inhibits abdominal aortic aneurysm in apolipoprotein E-knockout mice via collagen deposition and inflammatory inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Xingli Xu,Fang Zhang,Yue Lu,Sufang Yu,Wenqian Sun,Shangwen Sun,Jing Cheng,Jing Ma,Meng Zhang,Cheng Zhang,Yun Zhang,Kai Zhang

American journal of nephrology 41:48-56 PubMed25662584

2015

Renal C3 complement component: feed forward to diabetic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine J Kelly,Yunlong Liu,Jizhong Zhang,Jesus H Dominguez

Proteomics 10:3210-21 PubMed20707004

2010

Upregulation of plasma C9 protein in gastric cancer patients.

Applications

IHC-P, WB

Species

Human, Human

Poh-Kuan Chong,Huiyin Lee,Marie Chiew Shia Loh,Lee-Yee Choong,Qingsong Lin,Jimmy Bok Yan So,Khong Hee Lim,Ross Andrew Soo,Wei Peng Yong,Siew Pang Chan,Duane T Smoot,Hassan Ashktorab,Khay Guan Yeoh,Yoon Pin Lim

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-C9 antibody [53]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9 antibody [53] (AB17931)

Flow Cytometry - Anti-C9 antibody [53] (AB17931)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise