AB168345

Anti-C9 antibody [EPR11232]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal C9 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

Complement component C9, C9

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9 antibody [EPR11232] (AB168345)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9 antibody [EPR11232] (AB168345)

Immunohistochemical analysis of paraffin embedded Human lung tissue labeling C9 with ab168345 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-C9 antibody [EPR11232] (AB168345)

Immunoprecipitation : Western blot analysis on immunoprecipitation pellet from Human plasma cell lysate using ab168345 at a 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-C9 antibody [EPR11232] (ab168345) at 1/10 dilution

All lanes:

Human plasma cell lysate

Predicted band size: 63 kDa

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Unknown

Western blot - Anti-C9 antibody [EPR11232] (AB168345)

All lanes:

Anti-ZNF239 antibody (<a href='/en-us/products/unavailable/znf239-antibody-ab118345'>ab118345</a>) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

Human angioneoplasm lysate at 10 µg

Lane 3:

Human placenta lysate at 10 µg

Lane 4:

Human serum lysate at 10 µg

Lane 5:

Human plasma lysate at 10 µg

Predicted band size: 63 kDa

false

Carrier free

Anti-C9 antibody [EPR11232] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11232

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C9 also known as complement component 9 is a protein that plays an essential mechanical role in the complement system. It typically associates in the formation of the membrane attack complex (MAC). The C9 protein weighing around 71 kDa expresses in the liver and is secreted into the blood plasma. Alternative names like 'Cloud 9 capture' and 'C9 tag' often refer to research tools or motifs derived from the C9 protein rather than the protein itself. Researchers frequently study C9 in mouse models where its physiological roles can be explored.

Biological function summary

C9 participates in defending against pathogens. It joins other complement components like C5b C6 C7 and C8 to form the MAC complex which facilitates cell lysis by forming pores in the membrane of target cells. This action directly results in microbial cell destruction serving a critical immune function. C9 is not only part of the MAC complex but also holds significance due to its unique function as the last step in the formation of the pore influencing the efficacy of pathogen elimination.

Pathways

C9 functions primarily within the complement cascade which includes the classical and alternative pathways. Its role is in the terminal pathway segment that ultimately leads to the formation of the MAC and follows the activation of C5 which cleaves to produce C5b. This connection extends to proteins like C3 which is upstream in the cascade and significantly impacts the cascade's activation highlighting C9's function as an integral terminal component in these pathways.

Mutations or deficits in C9 relate to an increased susceptibility to infections particularly those caused by Neisseria species. This association results directly from its important role in the MAC formation. Additionally deficiency of C9 links to autoimmune disorders where the immune response may be improperly regulated due to compromised MAC formation. Other complement components like C6 and C8 may also relate to these diseases due to their collaboration with C9 in forming the MAC illustrating the interconnectedness of these proteins within the immune response framework.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Pore-forming component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis (PubMed : 22832194, PubMed : 26841837, PubMed : 26841934, PubMed : 27052168, PubMed : 30552328, PubMed : 6177822, PubMed : 9212048, PubMed : 9634479). The MAC is initiated by proteolytic cleavage of C5 into complement C5b in response to the classical, alternative, lectin and GZMK complement pathways (PubMed : 39914456, PubMed : 39814882, PubMed : 9212048, PubMed : 9634479). The complement pathways consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 9212048, PubMed : 9634479). Constitutes the pore-forming subunit of the MAC complex : during MAC assembly, C9 associates with the C5b8 intermediate complex, and polymerizes to complete the pore (PubMed : 26841934, PubMed : 30111885, PubMed : 30552328, PubMed : 34752492, PubMed : 4055801, PubMed : 6177822).

See full target information C9

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Clinical proteomics 22:12 PubMed40229672

2025

Proteomic profiles screening identified novel exosomal protein biomarkers for diagnosis of lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Weiyan Feng,Ying Lin,Ling Zhang,Weiming Hu

Renal failure 47:2440528 PubMed39806768

2025

IGFBP2 and IGFBP4 interact to activate complement pathway in diabetic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jieling Liang,Yangxiao Huang,Daping Peng,Yali Xie,Yifei Liu,Xiuxia Lu,Junfa Xu

Acta biochimica et biophysica Sinica 54:1694-1707 PubMed35929594

2022

Comprehensive analysis of complement-associated molecular features in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Run Huang,Guiqi Zhu,Xiutao Fu,Weiren Liu,Chenyang Tao,Jun Gao,Weifeng Qu,Yuan Fang,Xifei Jiang,Zhenbin Ding,Jian Zhou,Yinghong Shi,Jia Fan,Zheng Tang

Medical science monitor basic research 25:33-44 PubMed30713336

2019

Autophagy and Oncosis/Necroptosis Are Enhanced in Cardiomyocytes from Heart Failure Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Giovanni Corsetti,Carol Chen-Scarabelli,Claudia Romano,Evasio Pasini,Francesco S Dioguardi,Francesco Onorati,Richard Knight,Hemang Patel,Louis Saravolatz,Giuseppe Faggian,Tiziano M Scarabelli

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-C9 antibody [EPR11232]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9 antibody [EPR11232] (AB168345)

Immunoprecipitation - Anti-C9 antibody [EPR11232] (AB168345)

Western blot - Anti-C9 antibody [EPR11232] (AB168345)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise