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AB249445

Anti-C9 antibody [EPR11232] - BSA and Azide free

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Rabbit Recombinant Monoclonal C9 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples.

View Alternative Names

Complement component C9, C9

3 Images
Immunoprecipitation - Anti-C9 antibody [EPR11232] - BSA and Azide free (AB249445)
  • IP

Supplier Data

Immunoprecipitation - Anti-C9 antibody [EPR11232] - BSA and Azide free (AB249445)

This data was developed using ab168345, the same antibody clone in a different buffer formulation.

Immunoprecipitation : Western blot analysis on immunoprecipitation pellet from Human plasma cell lysate using ab168345 at a 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-C9 antibody [EPR11232] (<a href='/en-us/products/primary-antibodies/c9-antibody-epr11232-ab168345'>ab168345</a>) at 1/10 dilution

All lanes:

Human plasma cell lysate

Predicted band size: 63 kDa

false

Western blot - Anti-C9 antibody [EPR11232] - BSA and Azide free (AB249445)
  • WB

Unknown

Western blot - Anti-C9 antibody [EPR11232] - BSA and Azide free (AB249445)

This data was developed using ab168345, the same antibody clone in a different buffer formulation.

All lanes:

Anti-ZNF239 antibody (<a href='/en-us/products/unavailable/znf239-antibody-ab118345'>ab118345</a>) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

Human angioneoplasm lysate at 10 µg

Lane 3:

Human placenta lysate at 10 µg

Lane 4:

Human serum lysate at 10 µg

Lane 5:

Human plasma lysate at 10 µg

Predicted band size: 63 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9 antibody [EPR11232] - BSA and Azide free (AB249445)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9 antibody [EPR11232] - BSA and Azide free (AB249445)

This data was developed using ab168345, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human lung tissue labeling C9 with ab168345 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11232

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab249445 is the carrier-free version of ab168345.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C9 also known as complement component 9 is a protein that plays an essential mechanical role in the complement system. It typically associates in the formation of the membrane attack complex (MAC). The C9 protein weighing around 71 kDa expresses in the liver and is secreted into the blood plasma. Alternative names like 'Cloud 9 capture' and 'C9 tag' often refer to research tools or motifs derived from the C9 protein rather than the protein itself. Researchers frequently study C9 in mouse models where its physiological roles can be explored.
Biological function summary

C9 participates in defending against pathogens. It joins other complement components like C5b C6 C7 and C8 to form the MAC complex which facilitates cell lysis by forming pores in the membrane of target cells. This action directly results in microbial cell destruction serving a critical immune function. C9 is not only part of the MAC complex but also holds significance due to its unique function as the last step in the formation of the pore influencing the efficacy of pathogen elimination.

Pathways

C9 functions primarily within the complement cascade which includes the classical and alternative pathways. Its role is in the terminal pathway segment that ultimately leads to the formation of the MAC and follows the activation of C5 which cleaves to produce C5b. This connection extends to proteins like C3 which is upstream in the cascade and significantly impacts the cascade's activation highlighting C9's function as an integral terminal component in these pathways.

Mutations or deficits in C9 relate to an increased susceptibility to infections particularly those caused by Neisseria species. This association results directly from its important role in the MAC formation. Additionally deficiency of C9 links to autoimmune disorders where the immune response may be improperly regulated due to compromised MAC formation. Other complement components like C6 and C8 may also relate to these diseases due to their collaboration with C9 in forming the MAC illustrating the interconnectedness of these proteins within the immune response framework.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells (PubMed : 26841934, PubMed : 9212048, PubMed : 9634479). C9 is the pore-forming subunit of the MAC (PubMed : 26841934, PubMed : 30111885, PubMed : 4055801).
See full target information C9
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com