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AB97644

Anti-C9orf78 antibody

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(1 Publication)

Rabbit Polyclonal C9orf78 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human C9orf78 aa 1-250.

View Alternative Names

HCA59, C9orf78, Splicing factor C9orf78, Hepatocellular carcinoma-associated antigen 59

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9orf78 antibody (AB97644)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9orf78 antibody (AB97644)

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft tissue with ab97644 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunocytochemistry/ Immunofluorescence - Anti-C9orf78 antibody (AB97644)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-C9orf78 antibody (AB97644)

ab97644, at 1/200 dilution, staining C9orf78 in paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is merged with DNA probe.

Western blot - Anti-C9orf78 antibody (AB97644)
  • WB

Unknown

Western blot - Anti-C9orf78 antibody (AB97644)

12% SDS-PAGE

All lanes:

Western blot - Anti-C9orf78 antibody (ab97644) at 1/3000 dilution

Lane 1:

H1299 whole cell lysate at 30 µg

Lane 2:

HeLa S3 whole cell lysate at 30 µg

Lane 3:

HepG2 whole cell lysate at 30 µg

Predicted band size: 33 kDa

false

Western blot - Anti-C9orf78 antibody (AB97644)
  • WB

Supplier Data

Western blot - Anti-C9orf78 antibody (AB97644)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-C9orf78 antibody (ab97644) at 1/500 dilution

Lane 1:

PC-12 whole cell extracts at 30 µg

Lane 2:

Rat2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 34 kDa

false

Western blot - Anti-C9orf78 antibody (AB97644)
  • WB

Supplier Data

Western blot - Anti-C9orf78 antibody (AB97644)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-C9orf78 antibody (ab97644) at 1/500 dilution

Lane 1:

Neuro2a whole cell extracts at 30 µg

Lane 2:

C8D30 whole cell extracts at 30 µg

Lane 3:

NIH-3T3 whole cell extracts at 30 µg

Lane 4:

Raw264.7 whole cell extracts at 30 µg

Lane 5:

C2C12 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 34 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human C9orf78 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q9NZ63

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C9orf78 also known as chromosome 9 open reading frame 78 is a protein with a molecular mass of approximately 24 kDa. It is expressed in various tissues but shows higher levels in the lung and reproductive organs. On a mechanical level C9orf78 is involved in intracellular processes where it interacts with other cellular components indicating its role in cellular signaling and regulation.
Biological function summary

C9orf78 plays a role in cellular homeostasis and regulation of metabolic processes. It acts as part of a complex that modulates cellular responses to stress and can influence gene expression. The protein's involvement in these cellular processes suggests that it serves as a mediator between cellular signaling pathways and the physical expression of cellular responses.

Pathways

C9orf78 contributes significantly to the AMPK signaling pathway and interacts with various proteins including LKB1. This association positions it as an important factor in energy metabolism helping the cell respond to energy depletion. Its role in the cellular energy balance connects it closely to upstream and downstream signals that control cellular growth and metabolism making it important for maintaining cellular function.

C9orf78 is associated with complex conditions like metabolic syndrome and cancer. It links to disease progression possibly through its relationship with AMPK as dysregulation in this pathway can lead to metabolic imbalance and growth irregularities. Additionally its interaction with proteins like LKB1 known for its tumor suppressive functions further emphasizes the importance of C9orf78 in maintaining normal cellular operations and its potential impact when malfunctioning.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in pre-mRNA splicing by promoting usage of the upstream 3'-splice site at alternative NAGNAG splice sites; these are sites featuring alternative acceptor motifs separated by only a few nucleotides (PubMed : 35241646). May also modulate exon inclusion events (PubMed : 35241646). Plays a role in spliceosomal remodeling by displacing WBP4 from SNRNP200 and may act to inhibit SNRNP200 helicase activity (PubMed : 35241646). Binds U5 snRNA (PubMed : 35241646). Required for proper chromosome segregation (PubMed : 35167828). Not required for splicing of shelterin components (PubMed : 35167828).
See full target information C9orf78
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 40:1657-1665 PubMed30015890

2018

Homeobox containing 1 inhibits liver cancer progression by promoting autophagy as well as inhibiting stemness and immune escape.

Applications

Unspecified application

Species

Unspecified reactive species

Hengli Zhao,Huifeng Jia,Qiuju Han,Jian Zhang
View all publications
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