AB10481

Anti-CA150 antibody

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(1 Publication)

Rabbit Polyclonal CA150 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TCERG1 aa 1-50.

View Alternative Names

CA150, TAF2S, TCERG1, Transcription elongation regulator 1, TATA box-binding protein-associated factor 2S, Transcription factor CA150

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CA150 antibody (AB10481)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CA150 antibody (AB10481)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling CA150 at 1/1000 (1µg/ml). Detection : DAB.

Supplier Data

Western blot - Anti-CA150 antibody (AB10481)

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Western blot - Anti-CA150 antibody (ab10481) at 1/10000 dilution

Lane 1:

HeLa Whole cell lysate at 50 µg

Lane 2:

HEK293T Whole cell lysate at 50 µg

Predicted band size: 124 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human TCERG1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

O14776

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibodies were affinity purified using the peptide immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CA150 also known as TCERG1 (transcription elongation regulator 1) is a nuclear protein with a molecular mass of approximately 150 kDa. This protein localizes mainly in the nucleus where it is expressed in various cell types. Mechanically CA150 interacts with RNA polymerase II the enzyme responsible for transcribing DNA into messenger RNA. It regulates transcription elongation the phase where RNA polymerase II transcribes DNA into longer RNA strands. CA150 acts by modulating the phosphorylation of RNA polymerase II which is essential for progressing the transcription process from initiation to elongation.

Biological function summary

The transcription elongation regulator 1 protein modulates the expression of genes by being part of transcriptional complexes. CA150 has important interactions with the pre-mRNA splicing machinery impacting how precursor mRNAs are processed into mature mRNAs. This includes altering splicing patterns and affecting alternative splicing a process required for generating protein diversity. The protein influences cell cycle progression and cellular responses to DNA damage highlighting its important role in gene expression regulation and cellular homeostasis.

Pathways

Various genes and cellular processes show links to CA150's function. This protein is part of the transcriptional regulation pathway and is involved in the response to DNA damage signaling pathways. In these pathways CA150 directly interacts with proteins like cyclin-dependent kinases (CDKs) and the splicing factor SF3b to coordinate transcription and splicing processes as DNA is reproduced and repaired. Its ability to connect transcription with RNA processing makes CA150 integral to regulating gene expression at multiple stages.

Transcription elongation processes involving CA150 play a role in aberrant splicing events. Alterations in CA150 function are implicated in neurological disorders such as spinal muscular atrophy (SMA) where splicing defects occur. Moreover CA150 links with proteins like survival motor neuron (SMN) protein in these pathways. It also demonstrates connections to cancer where dysregulated gene expression and splicing defects can lead to tumorigenesis. Here modifying CA150 activity could influence oncogenic pathways suggesting that it might have potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that binds RNA polymerase II and inhibits the elongation of transcripts from target promoters. Regulates transcription elongation in a TATA box-dependent manner. Necessary for TAT-dependent activation of the human immunodeficiency virus type 1 (HIV-1) promoter.

See full target information TCERG1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bioscience reports 43: PubMed37782747

2023

Writers and readers of H3K9me2 form distinct protein networks during the cell cycle that include candidates for H3K9 mimicry.

Applications

Unspecified application

Species

Unspecified reactive species

Gareth Pollin,Thiago M De Assuncao,Salomao Doria Jorge,Young-In Chi,M Cristine Charlesworth,Benjamin Madden,Juan Iovanna,Michael T Zimmermann,Raul Urrutia,Gwen Lomberk

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CA150 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CA150 antibody (AB10481)

Western blot - Anti-CA150 antibody (AB10481)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

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Primary Antibodies

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Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise