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AB50812

Anti-CA150 antibody [TCEAD79A]

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(1 Publication)

Mouse Monoclonal CA150 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TCERG1.

View Alternative Names

CA150, TAF2S, TCERG1, Transcription elongation regulator 1, TATA box-binding protein-associated factor 2S, Transcription factor CA150

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-CA150 antibody [TCEAD79A] (AB50812)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CA150 antibody [TCEAD79A] (AB50812)

ab50812 at 1/10 dilution staining human TCERG1 in HeLa cells by Immunocytochemistry. Secondary antibody : 1/200 AlexaFluor®488 Goat Anti-mouse IgG.

Western blot - Anti-CA150 antibody [TCEAD79A] (AB50812)
  • WB

Unknown

Western blot - Anti-CA150 antibody [TCEAD79A] (AB50812)

All lanes:

Western blot - Anti-CA150 antibody [TCEAD79A] (ab50812) at 1/50 dilution

All lanes:

HeLa whole cell lysate at 50 µg

Secondary

All lanes:

anti-mouse IgG at 1/2500 dilution

Predicted band size: 124 kDa

true

Western blot - Anti-CA150 antibody [TCEAD79A] (AB50812)
  • WB

Unknown

Western blot - Anti-CA150 antibody [TCEAD79A] (AB50812)

All lanes:

Western blot - Anti-CA150 antibody [TCEAD79A] (ab50812) at 1/50 dilution

All lanes:

NIH3T3 whole cell lysate at 50 µg

Secondary

All lanes:

anti-mouse IgG at 1/2500 dilution

Predicted band size: 124 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

TCEAD79A

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human TCERG1. The exact immunogen used to generate this antibody is proprietary information.

O14776

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CA150 also known as TCERG1 (transcription elongation regulator 1) is a nuclear protein with a molecular mass of approximately 150 kDa. This protein localizes mainly in the nucleus where it is expressed in various cell types. Mechanically CA150 interacts with RNA polymerase II the enzyme responsible for transcribing DNA into messenger RNA. It regulates transcription elongation the phase where RNA polymerase II transcribes DNA into longer RNA strands. CA150 acts by modulating the phosphorylation of RNA polymerase II which is essential for progressing the transcription process from initiation to elongation.
Biological function summary

The transcription elongation regulator 1 protein modulates the expression of genes by being part of transcriptional complexes. CA150 has important interactions with the pre-mRNA splicing machinery impacting how precursor mRNAs are processed into mature mRNAs. This includes altering splicing patterns and affecting alternative splicing a process required for generating protein diversity. The protein influences cell cycle progression and cellular responses to DNA damage highlighting its important role in gene expression regulation and cellular homeostasis.

Pathways

Various genes and cellular processes show links to CA150's function. This protein is part of the transcriptional regulation pathway and is involved in the response to DNA damage signaling pathways. In these pathways CA150 directly interacts with proteins like cyclin-dependent kinases (CDKs) and the splicing factor SF3b to coordinate transcription and splicing processes as DNA is reproduced and repaired. Its ability to connect transcription with RNA processing makes CA150 integral to regulating gene expression at multiple stages.

Transcription elongation processes involving CA150 play a role in aberrant splicing events. Alterations in CA150 function are implicated in neurological disorders such as spinal muscular atrophy (SMA) where splicing defects occur. Moreover CA150 links with proteins like survival motor neuron (SMN) protein in these pathways. It also demonstrates connections to cancer where dysregulated gene expression and splicing defects can lead to tumorigenesis. Here modifying CA150 activity could influence oncogenic pathways suggesting that it might have potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that binds RNA polymerase II and inhibits the elongation of transcripts from target promoters. Regulates transcription elongation in a TATA box-dependent manner. Necessary for TAT-dependent activation of the human immunodeficiency virus type 1 (HIV-1) promoter.
See full target information TCERG1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature 466:138-42 PubMed20596027

2010

SCF(Cyclin F) controls centrosome homeostasis and mitotic fidelity through CP110 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Vincenzo D'Angiolella,Valerio Donato,Sangeetha Vijayakumar,Anita Saraf,Laurence Florens,Michael P Washburn,Brian Dynlacht,Michele Pagano
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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