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AB325744

Anti-CACHD1 antibody [15H10]

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Rat Recombinant Monoclonal CAHD1 antibody. Suitable for WB and reacts with Human, Mouse samples.

View Alternative Names

KIAA1573, VWCD1, VWFA and cache domain-containing protein 1, Cache domain-containing protein 1

3 Images
Western blot - Anti-CACHD1 antibody [15H10] (AB325744)
  • WB

Lab

Western blot - Anti-CACHD1 antibody [15H10] (AB325744)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the higher MW band at approximately 37 kDa (in lane 1, 2) is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-CACHD1 antibody [15H10] (ab325744) at 1/1000 dilution

Lane 1:

PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 50 µg

Lane 2:

PANC-1 transfected with siRNA specifically targeting CACHD1 whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/10000 dilution

Observed band size: 170 kDa,36 kDa

true

Exposure time: 180s

Western blot - Anti-CACHD1 antibody [15H10] (AB325744)
  • WB

Lab

Western blot - Anti-CACHD1 antibody [15H10] (AB325744)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : HT-29, HepG2, A20.

The identity of the bands between 15 kDa and 37 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-CACHD1 antibody [15H10] (ab325744) at 1/1000 dilution

Lane 1:

PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 40 µg

Lane 2:

SK-MEL-28 (human malignant melanoma cell ) whole cell lysate at 40 µg

Lane 3:

Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 40 µg

Lane 4:

HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 40 µg

Lane 5:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 40 µg

Lane 6:

F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 40 µg

Lane 7:

A20 (mouse reticulum sarcoma B lymphocyte) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/10000 dilution

Observed band size: 170 kDa,36 kDa

true

Exposure time: 180s

Western blot - Anti-CACHD1 antibody [15H10] (AB325744)
  • WB

Lab

Western blot - Anti-CACHD1 antibody [15H10] (AB325744)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression tissue : mouse lymph node

The identity of the lower MW band at approximately 50 kDa (in lane 1, 2) is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-CACHD1 antibody [15H10] (ab325744) at 1/1000 dilution

Lane 1:

mouse embryo tissue lysate at 20 µg

Lane 2:

mouse lymph node tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/10000 dilution

Observed band size: 170 kDa,36 kDa

true

Exposure time: 180s

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

15H10

Isotype

IgG1

Carrier free

No

Reacts with

Human, Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May regulate voltage-dependent calcium channels.
See full target information CACHD1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com