Anti-CACNA1A antibody

• IHC-FoFr

Collaborator

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-CACNA1A antibody (AB32642)

Immunofluorescent staining for CACNA1A using Rabbit polyclonal to CACNA1A (ab32642)in rat Purkinje (arrows) and granular (arrow heads) cells, of the rat cerebellum. Protocol details : Rats were intracardially perfused with 4% paraformaldehyde. Whole brain tissue was post-fixed overnight in the same fixative, and cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut by cyrostat for use in fre floating IHC. Primary antibody ab32642 was incubated overnight at 1/3000 at room temperature. Secondary antibody Alexa fluor 488 1/3000 was incubated for 2 hours at room temperature.

This image is courtesy of Sophie Pezet, Laboratoire de Neurobiologie, Paris, France

• WB

Lab

Western blot - Anti-CACNA1A antibody (AB32642)

Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

All lanes:

Western blot - Anti-CACNA1A antibody (ab32642) at 1 µg/mL

All lanes:

Cerebellum Rat Tissue Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 282 kDa

Observed band size: 190 kDa,56 kDa

false

• WB

Lab

Western blot - Anti-CACNA1A antibody (AB32642)

This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab32642 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-CACNA1A antibody (ab32642) at 1 µg/mL

Lane 1:

E16 Rat Embryo Brain - Tissue Lysate at 10 µg

Lane 2:

E16 Mouse Embryo Brain - Tissue Lysate at 10 µg

Lane 3:

E18 Rat Embryo Brain - Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 282 kDa

Observed band size: 190 kDa,42 kDa

true

Exposure time: 8min