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Rabbit Recombinant Monoclonal CACNA1C antibody. Suitable for IHC-Fr, IHC-P and reacts with Mouse samples.

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Images

Immunohistochemistry (Frozen sections) - Anti-CACNA1C antibody [EPR22625-61] (AB234438), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-CACNA1C antibody [EPR22625-61] (AB234438), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [EPR22625-61] (AB234438), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [EPR22625-61] (AB234438), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [EPR22625-61] (AB234438), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-FrIHC-P
Human
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Tested
Tested
Rat
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

-

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/4000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Rat, Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents (PubMed:14609949, PubMed:18586882, PubMed:21216955, PubMed:25368181, PubMed:28119464, PubMed:10973973). Mediates influx of calcium ions into the cytoplasm, and thereby triggers calcium release from the sarcoplasm (By similarity). Plays an important role in excitation-contraction coupling in the heart. Required for normal heart development and normal regulation of heart rhythm (PubMed:21216955). Required for normal contraction of smooth muscle cells in blood vessels and in the intestine. Essential for normal blood pressure regulation via its role in the contraction of arterial smooth muscle cells (PubMed:14609949, PubMed:28119464). Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group (Probable).

Alternative names

Cach2, Cacn2, Cacnl1a1, Cchl1a1, Cacna1c, Voltage-dependent L-type calcium channel subunit alpha-1C, MELC-CC, Mouse brain class C, Voltage-gated calcium channel subunit alpha Cav1.2, MBC

Recommended products

Rabbit Recombinant Monoclonal CACNA1C antibody. Suitable for IHC-Fr, IHC-P and reacts with Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22625-61
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CACNA1C also known as Cav1.2 is a voltage-dependent calcium channel α1C subunit. It primarily facilitates the influx of calcium ions into cells upon depolarization. Cav1.2 weighs approximately 250 kDa and plays an essential role in cellular processes across multiple systems. You can find it highly expressed in the heart brain and smooth muscle tissues. It's significant for conducting electrical signals that control muscle contractions and neurotransmitter release.

Biological function summary

Cav1.2 channels regulate intracellular calcium levels affecting processes such as cardiac contraction neuronal excitability and gene expression. This protein does not function alone; it forms complexes with other auxiliary subunits that modulate its activity and localization. Through this regulation CACNA1C supports critical cellular responses and maintains homeostasis within its expressed organs.

Pathways

Cav1.2 integrates into important pathways like the excitation-contraction coupling in cardiac myocytes and the neural signaling pathways in neurons. Through its interaction with calcium/calmodulin-dependent protein kinase II (CaMKII) CACNA1C affects downstream cascades that influence cellular functions and responses to external stimuli. The protein also interacts with other ion channels facilitating comprehensive cellular communication and function in its specialized tissues.

Associated diseases and disorders

Altered CACNA1C function associates with cardiac diseases like Timothy syndrome and various forms of Long QT syndrome. The abnormal activity of this channel also links to psychiatric disorders such as bipolar disorder where it interacts with proteins involved in mood regulation. Substantial research focuses on targeting CACNA1C for therapeutic intervention due to its significant role in these disorders highlighting the protein as an important target for new treatments.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Frozen sections) - Anti-CACNA1C antibody [EPR22625-61] (ab234438), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-CACNA1C antibody [EPR22625-61] (ab234438)

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling CACNA1C with ab234438 at 1/500 (0.87 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. Positive staining on mouse cerebrum is observed.

  • Immunohistochemistry (Frozen sections) - Anti-CACNA1C antibody [EPR22625-61] (ab234438), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-CACNA1C antibody [EPR22625-61] (ab234438)

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse cardiac muscle tissue labeling CACNA1C with ab234438 at 1/500 (0.87 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. Positive staining on mouse cardiac atrium myocytes and blood vessels throughout cardiac muscle (PMID: 26109061).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [EPR22625-61] (ab234438), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [EPR22625-61] (ab234438)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling CACNA1C with ab234438 at 1/4000 dilution (0.11 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on endothelial cells of blood vessels, muscularis mucosa and muscularis externa in mouse colon is observed. The section was incubated with ab234438 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [EPR22625-61] (ab234438), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [EPR22625-61] (ab234438)

    Immunohistochemical analysis of paraffin-embedded mouse ventricle tissue labeling CACNA1C with ab234438 at 1/2000 dilution (0.11 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the endothelial cells of blood vessels in mouse ventricle is observed (PMID: 26109061). The section was incubated with ab234438 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [EPR22625-61] (ab234438), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [EPR22625-61] (ab234438)

    Immunohistochemical analysis of paraffin-embedded mouse atrium tissue labeling CACNA1C with ab234438 at 1/4000 dilution (0.11 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on plasma membranes of atrial cardiomyocytes and endothelial cells of blood vessels is observed (PMID: 26109061). The section was incubated with ab234438 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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