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AB256543

Anti-CACNA1C antibody [EPR22625-61] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal CACNA1C antibody. Carrier free. Suitable for IHC-Fr, IHC-P and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

Cach2, Cacn2, Cacnl1a1, Cchl1a1, Cacna1c, Voltage-dependent L-type calcium channel subunit alpha-1C, MELC-CC, Mouse brain class C, Voltage-gated calcium channel subunit alpha Cav1.2, MBC

5 Images
Immunohistochemistry (Frozen sections) - Anti-CACNA1C antibody [EPR22625-61] - BSA and Azide free (AB256543)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-CACNA1C antibody [EPR22625-61] - BSA and Azide free (AB256543)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling CACNA1C with ab234438 at 1/500 (0.87 μg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. Positive staining on mouse cerebrum is observed.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234438).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [EPR22625-61] - BSA and Azide free (AB256543)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [EPR22625-61] - BSA and Azide free (AB256543)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling CACNA1C with ab234438 at 1/4000 dilution (0.11 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on endothelial cells of blood vessels, muscularis mucosa and muscularis externa in mouse colon is observed. The section was incubated with ab234438 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234438).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [EPR22625-61] - BSA and Azide free (AB256543)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [EPR22625-61] - BSA and Azide free (AB256543)

Immunohistochemical analysis of paraffin-embedded mouse atrium tissue labeling CACNA1C with ab234438 at 1/4000 dilution (0.11 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on plasma membranes of atrial cardiomyocytes and endothelial cells of blood vessels is observed (PMID : 26109061). The section was incubated with ab234438 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234438).

Immunohistochemistry (Frozen sections) - Anti-CACNA1C antibody [EPR22625-61] - BSA and Azide free (AB256543)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-CACNA1C antibody [EPR22625-61] - BSA and Azide free (AB256543)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse cardiac muscle tissue labeling CACNA1C with ab234438 at 1/500 (0.87 μg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. Positive staining on mouse cardiac atrium myocytes and blood vessels throughout cardiac muscle (PMID : 26109061).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234438).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [EPR22625-61] - BSA and Azide free (AB256543)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [EPR22625-61] - BSA and Azide free (AB256543)

Immunohistochemical analysis of paraffin-embedded mouse ventricle tissue labeling CACNA1C with ab234438 at 1/2000 dilution (0.11 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the endothelial cells of blood vessels in mouse ventricle is observed (PMID : 26109061). The section was incubated with ab234438 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234438).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22625-61

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

IHC-Fr, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab256543 is the carrier-free version of ab234438.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CACNA1C also known as Cav1.2 is a voltage-dependent calcium channel α1C subunit. It primarily facilitates the influx of calcium ions into cells upon depolarization. Cav1.2 weighs approximately 250 kDa and plays an essential role in cellular processes across multiple systems. You can find it highly expressed in the heart brain and smooth muscle tissues. It's significant for conducting electrical signals that control muscle contractions and neurotransmitter release.
Biological function summary

Cav1.2 channels regulate intracellular calcium levels affecting processes such as cardiac contraction neuronal excitability and gene expression. This protein does not function alone; it forms complexes with other auxiliary subunits that modulate its activity and localization. Through this regulation CACNA1C supports critical cellular responses and maintains homeostasis within its expressed organs.

Pathways

Cav1.2 integrates into important pathways like the excitation-contraction coupling in cardiac myocytes and the neural signaling pathways in neurons. Through its interaction with calcium/calmodulin-dependent protein kinase II (CaMKII) CACNA1C affects downstream cascades that influence cellular functions and responses to external stimuli. The protein also interacts with other ion channels facilitating comprehensive cellular communication and function in its specialized tissues.

Altered CACNA1C function associates with cardiac diseases like Timothy syndrome and various forms of Long QT syndrome. The abnormal activity of this channel also links to psychiatric disorders such as bipolar disorder where it interacts with proteins involved in mood regulation. Substantial research focuses on targeting CACNA1C for therapeutic intervention due to its significant role in these disorders highlighting the protein as an important target for new treatments.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents (PubMed : 14609949, PubMed : 18586882, PubMed : 21216955, PubMed : 25368181, PubMed : 28119464, PubMed : 10973973). Mediates influx of calcium ions into the cytoplasm, and thereby triggers calcium release from the sarcoplasm (By similarity). Plays an important role in excitation-contraction coupling in the heart. Required for normal heart development and normal regulation of heart rhythm (PubMed : 21216955). Required for normal contraction of smooth muscle cells in blood vessels and in the intestine. Essential for normal blood pressure regulation via its role in the contraction of arterial smooth muscle cells (PubMed : 14609949, PubMed : 28119464). Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group (Probable).
See full target information Cacna1c
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 5:101791 PubMed39426375

2024

Schwann cell-secreted frizzled-related protein 1 dictates neuroinflammation and peripheral nerve degeneration after neurotrauma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyun Yao,Lingchi Kong,Yi Qiao,David Brand,Juehong Li,Zhiwen Yan,Song Guo Zheng,Yun Qian,Cunyi Fan
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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