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AB283581

Anti-CACNA1C antibody [EPR24535-131] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal CACNA1C antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Cach2, Cacn2, Cacnl1a1, Cchl1a1, Cacna1c, Voltage-dependent L-type calcium channel subunit alpha-1C, MELC-CC, Mouse brain class C, Voltage-gated calcium channel subunit alpha Cav1.2, MBC

1 Images
Western blot - Anti-CACNA1C antibody [EPR24535-131] - BSA and Azide free (AB283581)
  • WB

Supplier Data

Western blot - Anti-CACNA1C antibody [EPR24535-131] - BSA and Azide free (AB283581)

This data was developed using ab270987, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 7081980)

Negative control : liver (PMID : 15863612)

Samples were un-boiled.

Exposure time : 103 seconds

All lanes:

Western blot - Anti-CACNA1C antibody [EPR24535-131] - BSA and Azide free (ab283581) at 1/1000 dilution

Lane 1:

Mouse hippocampus tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Mouse liver tissue lysate at 20 µg

Lane 4:

Rat hippocampus tissue lysate at 20 µg

Lane 5:

Rat heart tissue lysate at 20 µg

Lane 6:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 248 kDa

Observed band size: 240 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24535-131

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab283581 is the carrier-free version of ab270987.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CACNA1C also known as Cav1.2 is a voltage-dependent calcium channel α1C subunit. It primarily facilitates the influx of calcium ions into cells upon depolarization. Cav1.2 weighs approximately 250 kDa and plays an essential role in cellular processes across multiple systems. You can find it highly expressed in the heart brain and smooth muscle tissues. It's significant for conducting electrical signals that control muscle contractions and neurotransmitter release.
Biological function summary

Cav1.2 channels regulate intracellular calcium levels affecting processes such as cardiac contraction neuronal excitability and gene expression. This protein does not function alone; it forms complexes with other auxiliary subunits that modulate its activity and localization. Through this regulation CACNA1C supports critical cellular responses and maintains homeostasis within its expressed organs.

Pathways

Cav1.2 integrates into important pathways like the excitation-contraction coupling in cardiac myocytes and the neural signaling pathways in neurons. Through its interaction with calcium/calmodulin-dependent protein kinase II (CaMKII) CACNA1C affects downstream cascades that influence cellular functions and responses to external stimuli. The protein also interacts with other ion channels facilitating comprehensive cellular communication and function in its specialized tissues.

Altered CACNA1C function associates with cardiac diseases like Timothy syndrome and various forms of Long QT syndrome. The abnormal activity of this channel also links to psychiatric disorders such as bipolar disorder where it interacts with proteins involved in mood regulation. Substantial research focuses on targeting CACNA1C for therapeutic intervention due to its significant role in these disorders highlighting the protein as an important target for new treatments.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents (PubMed : 14609949, PubMed : 18586882, PubMed : 21216955, PubMed : 25368181, PubMed : 28119464, PubMed : 10973973). Mediates influx of calcium ions into the cytoplasm, and thereby triggers calcium release from the sarcoplasm (By similarity). Plays an important role in excitation-contraction coupling in the heart. Required for normal heart development and normal regulation of heart rhythm (PubMed : 21216955). Required for normal contraction of smooth muscle cells in blood vessels and in the intestine. Essential for normal blood pressure regulation via its role in the contraction of arterial smooth muscle cells (PubMed : 14609949, PubMed : 28119464). Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group (Probable).
See full target information Cacna1c
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BMC cardiovascular disorders 24:519 PubMed39333891

2024

The interaction between neutrophils and atrial myocytes in the occurrence and development of atrial fibrillation.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Li,Yan Wang,Jie Wan,Yu Duan,Yang Xu,Mao Yang
View all publications
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