AB84814

Anti-CACNA1C antibody [S57]

Name: Anti-CACNA1C antibody [S57] (ab84814) | Abcam
Brand: Abcam Limited
SKU: AB84814
Price: 560 USD
Availability: InStock
Rating: 5 (6 reviews)

(6 Reviews)

(39 Publications)

Anti-CACNA1C antibody [S57] (ab84814) is a mouse monoclonal antibody detecting CACNA1C in Western Blot, Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse,.

- Recombinant format for unrivalled batch-batch consistency
- Over 20 publications
- Trusted since 2009

View Alternative Names

CACH2, CACN2, CACNL1A1, CCHL1A1, CACNA1C, Voltage-dependent L-type calcium channel subunit alpha-1C, Voltage-gated calcium channel subunit alpha Cav1.2

5 Images

Flow Cytometry - Anti-CACNA1C antibody [S57] (AB84814)

Flow Cyt

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Flow Cytometry - Anti-CACNA1C antibody [S57] (AB84814)

Overlay histogram showing SH-SY5Y cells stained with ab84814 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84814, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [S57] (AB84814)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [S57] (AB84814)

ab84814 staining CACNA1C in human hippocampus tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin embedded tissue sections). Tissue underwent heat mediate fixation in microwave and in citrate buffer. The primary antibody was used at 1/100 dilution. A Fluorophore conjugated goat anti-mouse was used as secondary at 1/50 dilution.

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [S57] (AB84814)

ab84814 staining CACNA1C in mouse brain tissue section by immunohistochemistry (Bouin's fixed paraffin embedded tissue section. Tissue underwent heat mediated fixation in microwave and in citrate buffer. The primary antibody was used at 1/100 dilution. A HRP conjugated secondary was used at 10 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-CACNA1C antibody [S57] (AB84814)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CACNA1C antibody [S57] (AB84814)

Immunofluorescent analysis of 4% paraformaldehyde-fixed SH-SY5Y (Human Neuroblastoma Cell Line) cells labelling CACNA1C with ab84814 at 1/50 dilution, followed by Alexa Fluor® 488 anti Mouse secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on SH-SY5Y. Counterstain : Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1 : 800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) CACNA1C Antibody (D) Composite image.

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Western blot - Anti-CACNA1C antibody [S57] (AB84814)

All lanes:

Western blot - Anti-CACNA1C antibody [S57] (ab84814) at 1 µg/mL

All lanes:

Cell lysates prepared from DHPR alpha 1 transfected CHO cells

Predicted band size: 248 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

S57

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human

Applications

WB, Flow Cyt, ICC/IF, IHC-P

applications

Immunogen

Recombinant Protein within Rabbit CACNA1C aa 1500-1750. The exact immunogen used to generate this antibody is proprietary information.

P15381

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.1-10 µg/mL", "IHCP-species-notes": "Use at 0.1-1.0μg/mL (perox), 1.0-10μg/mL (IF).", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.1-10 µg/mL", "IHCP-species-notes": "Use at 0.1-1.0μg/mL (perox), 1.0-10μg/mL (IF).", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell lysate - Rabbit": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "If results are poor, use lysate without boiling, heat at 37°C for 15 minutes.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-CACNA1C antibody [S57] (ab84814) is a mouse recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, samples.

Trusted by the scientific community
Anti-CACNA1C [S57] (ab84814) was first used in a scientific publication in 2009 and has been cited over 20 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CACNA1C also known as Cav1.2 is a voltage-dependent calcium channel α1C subunit. It primarily facilitates the influx of calcium ions into cells upon depolarization. Cav1.2 weighs approximately 250 kDa and plays an essential role in cellular processes across multiple systems. You can find it highly expressed in the heart brain and smooth muscle tissues. It's significant for conducting electrical signals that control muscle contractions and neurotransmitter release.

Biological function summary

Cav1.2 channels regulate intracellular calcium levels affecting processes such as cardiac contraction neuronal excitability and gene expression. This protein does not function alone; it forms complexes with other auxiliary subunits that modulate its activity and localization. Through this regulation CACNA1C supports critical cellular responses and maintains homeostasis within its expressed organs.

Pathways

Cav1.2 integrates into important pathways like the excitation-contraction coupling in cardiac myocytes and the neural signaling pathways in neurons. Through its interaction with calcium/calmodulin-dependent protein kinase II (CaMKII) CACNA1C affects downstream cascades that influence cellular functions and responses to external stimuli. The protein also interacts with other ion channels facilitating comprehensive cellular communication and function in its specialized tissues.

Altered CACNA1C function associates with cardiac diseases like Timothy syndrome and various forms of Long QT syndrome. The abnormal activity of this channel also links to psychiatric disorders such as bipolar disorder where it interacts with proteins involved in mood regulation. Substantial research focuses on targeting CACNA1C for therapeutic intervention due to its significant role in these disorders highlighting the protein as an important target for new treatments.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents (PubMed : 12181424, PubMed : 15454078, PubMed : 15863612, PubMed : 16299511, PubMed : 17224476, PubMed : 20953164, PubMed : 23677916, PubMed : 24728418, PubMed : 26253506, PubMed : 27218670, PubMed : 29078335, PubMed : 29742403, PubMed : 30023270, PubMed : 30172029, PubMed : 34163037, PubMed : 8099908). Mediates influx of calcium ions into the cytoplasm, and thereby triggers calcium release from the sarcoplasm (By similarity). Plays an important role in excitation-contraction coupling in the heart. Required for normal heart development and normal regulation of heart rhythm (PubMed : 15454078, PubMed : 15863612, PubMed : 17224476, PubMed : 24728418, PubMed : 26253506). Required for normal contraction of smooth muscle cells in blood vessels and in the intestine. Essential for normal blood pressure regulation via its role in the contraction of arterial smooth muscle cells (PubMed : 28119464). Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group (Probable).. Isoform 12. Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents.. Isoform 13. Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents.. Isoform 14. Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents.. Isoform 15. Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents.. Isoform 16. Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents.. Isoform 17. Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents.. Isoform 18. Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents.. Isoform 19. Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents.. Isoform 20. Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents.. Isoform 21. Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents.. Isoform 22. Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents.. Isoform 23. Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents.. Isoform 24. Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents.. Isoform 25. Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents.. Isoform 26. Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents.. Isoform 27. Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents.. Isoform 34. Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents.. (Microbial infection) Acts as a receptor for Influenzavirus (PubMed : 29779930). May play a critical role in allowing virus entry when sialylated and expressed on lung tissues (PubMed : 29779930).

See full target information CACNA1C

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Publications (39)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in nutrition 12:1647017 PubMed41019540

2025

Taohong Siwu Decoction alleviates high salt-induced calcium overload and ferroptosis in vascular endothelial cells in hypertension by regulating ATF4.

Applications

Unspecified application

Species

Unspecified reactive species

Lai Kwan Lam,Peng-Li Xu,Peng-Cheng Xie,Qiu-Er Liang,Ting Xie,Lee Yam Poon,Ya Xiao,Li-Guo Chen

Frontiers in cell and developmental biology 13:1499339 PubMed40510743

2025

Embryo-derived trypsin-induced calcium entry is inhibited by endometrial infertility factor, LEFTY2.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqi Yang,Jing Yan,Steffen Kull,Md Alauddin,Sara Y Brucker,Melanie Henes,Florian Lang,Madhuri S Salker

The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed39299804

2024

Glucocorticoids Rapidly Modulate Ca1.2-Mediated Calcium Signals through Kv2.1 Channel Clusters in Hippocampal Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Di Wan,Tongchuang Lu,Chenyang Li,Changlong Hu

American journal of physiology. Cell physiology 326:C1237-C1247 PubMed38581667

2024

Extracellular glucose and dysfunctional insulin receptor signaling independently upregulate arterial smooth muscle TMEM16A expression.

Applications

Unspecified application

Species

Unspecified reactive species

Somasundaram Raghavan,Masuma Akter Brishti,Angelica Bernardelli,Alejandro Mata-Daboin,Jonathan H Jaggar,M Dennis Leo

Phytotherapy research : PTR 38:1044-1058 PubMed38153125

2023

Astilbin protects from sepsis-induced cardiac injury through the NRF2/HO-1 and TLR4/NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao Fang,Guangji Wang,Rui Huang,Chengyin Liu,Feierkaiti Yushanjiang,Tuohua Mao,Jun Li

Environmental toxicology 39:357-366 PubMed37755144

2023

MiRNA-26a-5p inhibits preterm labor initiation by targeting and regulating TRPC3 ion channel protein expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Chen,Tong Liu,Hong Cui,Quan Na,Sishi Liu

Orphanet journal of rare diseases 18:205 PubMed37542348

2023

PAI1 inhibits the pathogenesis of primary focal hyperhidrosis by targeting CHRNA1.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Feng Chen,Min Lin,Xu Li,Jian-Bo Lin

International journal of biological sciences 19:2934-2956 PubMed37324937

2023

Colchicine attenuates the electrical remodeling of post-operative atrial fibrillation through inhibited expression of immune-related hub genes and stabilization of microtubules.

Applications

Unspecified application

Species

Unspecified reactive species

Hangying Ying,Wenpu Guo,Xiaomei Tang,Jun Pan,Pengcheng Yu,Hangping Fan,Xiaochen Wang,Ruhong Jiang,Chenyang Jiang,Ping Liang

International journal of molecular sciences 24: PubMed37047701

2023

The Effect of CaV1.2 Inhibitor Nifedipine on Chondrogenic Differentiation of Human Bone Marrow or Menstrual Blood-Derived Mesenchymal Stem Cells and Chondrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Ilona Uzieliene,Daiva Bironaite,Rokas Miksiunas,Edvardas Bagdonas,Raminta Vaiciuleviciute,Ali Mobasheri,Eiva Bernotiene

Neural regeneration research 18:1570-1577 PubMed36571364

2022

L- and T-type Ca channels dichotomously contribute to retinal ganglion cell injury in experimental glaucoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Ning Wang,Wen-Jing Qian,Guo-Li Zhao,Fang Li,Yan-Ying Miao,Bo Lei,Xing-Huai Sun,Zhong-Feng Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CACNA1C antibody [S57]

Flow Cytometry - Anti-CACNA1C antibody [S57] (AB84814)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [S57] (AB84814)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [S57] (AB84814)

Immunocytochemistry/ Immunofluorescence - Anti-CACNA1C antibody [S57] (AB84814)

Western blot - Anti-CACNA1C antibody [S57] (AB84814)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (39)

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