Mouse Recombinant Monoclonal CACNA1C antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Transfected cell lysate - Rabbit samples. Cited in 28 publications. Immunogen corresponding to Recombinant Protein within Rabbit CACNA1C aa 1500-1750.
Images
![Western blot - Anti-CACNA1C antibody [S57] (AB84814), expandable thumbnail](https://content.abcam.com/products/images/cacna1c-antibody-s57-ab84814--western-blot-img161.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CACNA1C antibody [S57] (AB84814)")
![Flow Cytometry - Anti-CACNA1C antibody [L57/46] (AB84814), expandable thumbnail](https://content.abcam.com/products/images/cacna1c-antibody-s57-ab84814--flow-cytometry-img50584.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CACNA1C antibody [L57/46] (AB84814)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [L57/46] (AB84814), expandable thumbnail](https://content.abcam.com/products/images/cacna1c-antibody-s57-ab84814--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81128.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [L57/46] (AB84814)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [L57/46] (AB84814), expandable thumbnail](https://content.abcam.com/products/images/cacna1c-antibody-s57-ab84814--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img29186.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [L57/46] (AB84814)")
![Immunocytochemistry/ Immunofluorescence - Anti-CACNA1C antibody [L57/46] (AB84814), expandable thumbnail](https://content.abcam.com/products/images/cacna1c-antibody-s57-ab84814--immunocytochemistry-immunofluorescence-img72300.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CACNA1C antibody [L57/46] (AB84814)")
Publications
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- Environmental toxicology 39:357-3662023MiRNA-26a-5p inhibits preterm labor initiation by targeting and regulating TRPC3 ion channel protein expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Chen,Tong Liu,Hong Cui,Quan Na,Sishi LiuPubMed 37755144
- Orphanet journal of rare diseases 18:2052023PAI1 inhibits the pathogenesis of primary focal hyperhidrosis by targeting CHRNA1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian-Feng Chen,Min Lin,Xu Li,Jian-Bo LinPubMed 37542348
- International journal of biological sciences 19:2934-29562023Colchicine attenuates the electrical remodeling of post-operative atrial fibrillation through inhibited expression of immune-related hub genes and stabilization of microtubules.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHangying Ying,Wenpu Guo,Xiaomei Tang,Jun Pan,Pengcheng Yu,Hangping Fan,Xiaochen Wang,Ruhong Jiang,Chenyang Jiang,Ping LiangPubMed 37324937
- International journal of molecular sciences 24:2023The Effect of CaV1.2 Inhibitor Nifedipine on Chondrogenic Differentiation of Human Bone Marrow or Menstrual Blood-Derived Mesenchymal Stem Cells and Chondrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIlona Uzieliene,Daiva Bironaite,Rokas Miksiunas,Edvardas Bagdonas,Raminta Vaiciuleviciute,Ali Mobasheri,Eiva BernotienePubMed 37047701
- Viruses 14:2022Nisoldipine Inhibits Influenza A Virus Infection by Interfering with Virus Internalization Process.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYingna Huang,Yinyan Li,Zhixuan Chen,Liurong Chen,Jinlong Liang,Chunyu Zhang,Zhengyin Zhang,Jie YangPubMed 36560742
- JOR spine 5:e12132022L-type Voltage-Gated calcium channels partly mediate Mechanotransduction in the intervertebral disc.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPhilip Poillot,Joseph W Snuggs,Christine L Le Maitre,Jacques M HuyghePubMed 36601377
- Bioengineered 13:8593-86042022MCC950 ameliorates ventricular arrhythmia vulnerability induced by heart failure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaobo Jiang,Fan Yang,Dengke Ou,Luyong Huang,Hongfei Li,Mingjian LangPubMed 35287557
- Frontiers in cardiovascular medicine 8:7906122022IL-6/STAT3 Signaling Promotes Cardiac Dysfunction by Upregulating FUNDC1-Dependent Mitochondria-Associated Endoplasmic Reticulum Membranes Formation in Sepsis Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Jiang,Dewei Peng,Wei Shi,Junyi Guo,Shengqi Huo,Lintong Men,Cuntai Zhang,Sheng Li,Jiagao Lv,Li LinPubMed 35118141
- Aging and disease 13:298-3122022Atrial Fibrillation Underlies Cardiomyocyte Senescence and Contributes to Deleterious Atrial Remodeling during Disease Progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAiliya Adili,Xiyu Zhu,Hailong Cao,Xinlong Tang,Yali Wang,Junxia Wang,Jian Shi,Qing Zhou,Dongjin WangPubMed 35111375
- Toxicology and applied pharmacology 410:1153612020Astragaloside IV protects against retinal iron overload toxicity through iron regulation and the inhibition of MAPKs and NF-κB activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiongtao Song,Ying Zhao,Yanrong Yang,Xue Han,Junguo DuanPubMed 33285147
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
Preservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Protein within Rabbit CACNA1C aa 1500-1750. The exact immunogen used to generate this antibody is proprietary information. Database link P15381
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested |
| Mouse | Expected | Expected | Tested | Expected |
| Transfected cell lysate - Rabbit | Not recommended | Tested | Not recommended | Not recommended |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg for 106 Cells | Notes ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Rabbit | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Rabbit | Dilution info 1 µg/mL | Notes If results are poor, use lysate without boiling, heat at 37°C for 15 minutes. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.10000-10.00000 µg/mL | Notes Use at 0.1-1.0μg/mL (perox), 1.0-10μg/mL (IF). |
Species Human | Dilution info 0.10000-10.00000 µg/mL | Notes Use at 0.1-1.0μg/mL (perox), 1.0-10μg/mL (IF). |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Rabbit | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Rabbit | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents (PubMed:8392192, PubMed:7737988, PubMed:9087614, PubMed:9013606, PubMed:9607315, PubMed:12176756, PubMed:17071743, PubMed:11741969, PubMed:8099908, PubMed:12181424, PubMed:29078335, PubMed:29742403, PubMed:16299511, PubMed:20953164, PubMed:15454078, PubMed:15863612, PubMed:17224476, PubMed:24728418, PubMed:26253506, PubMed:27218670, PubMed:23677916). Mediates influx of calcium ions into the cytoplasm, and thereby triggers calcium release from the sarcoplasm (By similarity). Plays an important role in excitation-contraction coupling in the heart. Required for normal heart development and normal regulation of heart rhythm (PubMed:15454078, PubMed:15863612, PubMed:17224476, PubMed:24728418, PubMed:26253506). Required for normal contraction of smooth muscle cells in blood vessels and in the intestine. Essential for normal blood pressure regulation via its role in the contraction of arterial smooth muscle cells (PubMed:28119464). Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group (Probable).(Microbial infection) Acts as a receptor for Influenzavirus (PubMed:29779930). May play a critical role in allowing virus entry when sialylated and expressed on lung tissues (PubMed:29779930).
Alternative names
Voltage-dependent L-type calcium channel subunit alpha-1C, Voltage-gated calcium channel subunit alpha Cav1.2, CACNL1A1, CACN2, CACH2, CACNA1C, CCHL1A1
Recommended products
Mouse Recombinant Monoclonal CACNA1C antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Transfected cell lysate - Rabbit samples. Cited in 28 publications. Immunogen corresponding to Recombinant Protein within Rabbit CACNA1C aa 1500-1750.
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
Preservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Protein within Rabbit CACNA1C aa 1500-1750. The exact immunogen used to generate this antibody is proprietary information. Database link P15381
- Clone number
S57
- Purification technique
Affinity purification Protein G
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
The clone number has been updated from S57-46 to L57/46, both clone numbers name the same antibody clone.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CACNA1C also known as Cav1.2 is a voltage-dependent calcium channel α1C subunit. It primarily facilitates the influx of calcium ions into cells upon depolarization. Cav1.2 weighs approximately 250 kDa and plays an essential role in cellular processes across multiple systems. You can find it highly expressed in the heart brain and smooth muscle tissues. It's significant for conducting electrical signals that control muscle contractions and neurotransmitter release.
- Biological function summary
Cav1.2 channels regulate intracellular calcium levels affecting processes such as cardiac contraction neuronal excitability and gene expression. This protein does not function alone; it forms complexes with other auxiliary subunits that modulate its activity and localization. Through this regulation CACNA1C supports critical cellular responses and maintains homeostasis within its expressed organs.
- Pathways
Cav1.2 integrates into important pathways like the excitation-contraction coupling in cardiac myocytes and the neural signaling pathways in neurons. Through its interaction with calcium/calmodulin-dependent protein kinase II (CaMKII) CACNA1C affects downstream cascades that influence cellular functions and responses to external stimuli. The protein also interacts with other ion channels facilitating comprehensive cellular communication and function in its specialized tissues.
- Associated diseases and disorders
Altered CACNA1C function associates with cardiac diseases like Timothy syndrome and various forms of Long QT syndrome. The abnormal activity of this channel also links to psychiatric disorders such as bipolar disorder where it interacts with proteins involved in mood regulation. Substantial research focuses on targeting CACNA1C for therapeutic intervention due to its significant role in these disorders highlighting the protein as an important target for new treatments.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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5 product images
![Western blot - Anti-CACNA1C antibody [S57] (ab84814), expandable thumbnail](https://content.abcam.com/products/images/cacna1c-antibody-s57-ab84814--western-blot-img161.jpg "Western blot - Anti-CACNA1C antibody [S57] (ab84814)")
Western blot - Anti-CACNA1C antibody [S57] (ab84814)
All lanes: Western blot - Anti-CACNA1C antibody [S57] (ab84814) at 1 µg/mL
All lanes: Cell lysates prepared from DHPR alpha 1 transfected CHO cells
Predicted band size: 248 kDa
![Flow Cytometry - Anti-CACNA1C antibody [L57/46] (ab84814), expandable thumbnail](https://content.abcam.com/products/images/cacna1c-antibody-s57-ab84814--flow-cytometry-img50584.jpg "Flow Cytometry - Anti-CACNA1C antibody [L57/46] (ab84814)")
Flow Cytometry - Anti-CACNA1C antibody [L57/46] (ab84814)
Overlay histogram showing SH-SY5Y cells stained with ab84814 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84814, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (Mouse IgG2b [PLPV219] - Isotype Control ab91366, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [L57/46] (ab84814), expandable thumbnail](https://content.abcam.com/products/images/cacna1c-antibody-s57-ab84814--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81128.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [L57/46] (ab84814)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [L57/46] (ab84814)
ab84814 staining CACNA1C in human hippocampus tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin embedded tissue sections). Tissue underwent heat mediate fixation in microwave and in citrate buffer. The primary antibody was used at 1/100 dilution. A Fluorophore conjugated goat anti-mouse was used as secondary at 1/50 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [L57/46] (ab84814), expandable thumbnail](https://content.abcam.com/products/images/cacna1c-antibody-s57-ab84814--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img29186.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [L57/46] (ab84814)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [L57/46] (ab84814)
ab84814 staining CACNA1C in mouse brain tissue section by immunohistochemistry (Bouin's fixed paraffin embedded tissue section. Tissue underwent heat mediated fixation in microwave and in citrate buffer. The primary antibody was used at 1/100 dilution. A HRP conjugated secondary was used at 10 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-CACNA1C antibody [L57/46] (ab84814), expandable thumbnail](https://content.abcam.com/products/images/cacna1c-antibody-s57-ab84814--immunocytochemistry-immunofluorescence-img72300.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CACNA1C antibody [L57/46] (ab84814)")
Immunocytochemistry/ Immunofluorescence - Anti-CACNA1C antibody [L57/46] (ab84814)
Formaldehyde-fixed SK-N-BE cells stained for CACNA1C (green) using ab84814 at 1/100 dilution in ICC/IF.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/200 dilution for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain (A); DAPI (B, blue) nuclear stain at 1/1000, 1/5000 for 60 minutes at room temperature, 5 minutes at room temperature.
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