AB171968

Anti-CACNA1F antibody [EPR11822]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal CACNA1F antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CACNAF1, CACNA1F, Voltage-dependent L-type calcium channel subunit alpha-1F, Voltage-gated calcium channel subunit alpha Cav1.4

2 Images

Flow Cytometry - Anti-CACNA1F antibody [EPR11822] (AB171968)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CACNA1F antibody [EPR11822] (AB171968)

Flow cytometric analysis of Y79 cells labeling CACNA1F using ab171968 at a 1/100 dilution (red) or a rabbit IgG negative control (green).

Supplier Data

Western blot - Anti-CACNA1F antibody [EPR11822] (AB171968)

All lanes:

Western blot - Anti-CACNA1F antibody [EPR11822] (ab171968) at 1/1000 dilution

Lane 1:

A673 cell lysate at 10 µg

Lane 2:

Y79 cell lysate at 10 µg

Secondary

All lanes:

Standard HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 219 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11822

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100 - 1/500", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CACNA1F gene encodes the alpha-1F subunit of a voltage-dependent L-type calcium channel. This protein also known as Cav1.4 has a mass of approximately 230 kDa. It facilitates the influx of calcium ions into the cells in response to changes in electric potential across the cell membrane. The protein is expressed mainly in the retina where it plays an important role in the function of photoreceptor cells.

Biological function summary

The CACNA1F protein influences neurotransmitter release by regulating calcium entry which is essential for visual processing. It functions as a part of a larger voltage-gated calcium channel complex where its role is to fine-tune calcium signals. This precise control is necessary for the proper response to light stimuli in the eye allowing effective signal transmission to the visual pathways.

Pathways

The CACNA1F protein integrates into the calcium signaling pathway important for neuronal communication and sensory processes. It is also linked to the phototransduction cascade in the retina. Within these pathways CACNA1F interacts with related proteins like beta subunits that modulate the activity of the L-type calcium channels impacting overall visual function.

Mutations in the CACNA1F gene are associated with X-linked congenital stationary night blindness and other retinal disorders. These conditions can lead to an impaired vision due to the disrupted calcium flow in photoreceptors. Additionally CACNA1F shows a relationship with other calcium channel proteins like CACNA1S potentially influencing disorder phenotypes through altered ionic homeostasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Isoform 1. Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1F gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, and by benzothiazepines. Activates at more negative voltages and does not undergo calcium-dependent inactivation (CDI), due to incoming calcium ions, during depolarization.. Isoform 4. Voltage-dependent L-type calcium channel activates at more hyperpolarized voltages and exhibits a robust calcium-dependent inactivation (CDI), due to incoming calcium ions, during depolarizations.. Isoform 5. Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death.. Isoform 6. Voltage-dependent L-type calcium channel activates at more hyperpolarized voltages and exhibits a robust calcium-dependent inactivation (CDI), due to incoming calcium ions, during depolarizations.

See full target information CACNA1F

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Acta pharmacologica Sinica 38:1663-1672 PubMed28713161

2017

Functional expression of the Ca signaling machinery in human embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Jun Huang,Yi-Jie Wang,Min Zhang,Peng Zhang,He Liang,Hua-Jun Bai,Xiu-Jian Yu,Huang-Tian Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CACNA1F antibody [EPR11822]

Flow Cytometry - Anti-CACNA1F antibody [EPR11822] (AB171968)

Western blot - Anti-CACNA1F antibody [EPR11822] (AB171968)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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