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AB249680

Anti-CACNA1F antibody [EPR11822] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal CACNA1F antibody. Carrier free. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CACNAF1, CACNA1F, Voltage-dependent L-type calcium channel subunit alpha-1F, Voltage-gated calcium channel subunit alpha Cav1.4

2 Images
Flow Cytometry - Anti-CACNA1F antibody [EPR11822] - BSA and Azide free (AB249680)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CACNA1F antibody [EPR11822] - BSA and Azide free (AB249680)

This data was developed using ab171968, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Y79 cells, labeling CACNA1F using ab171968 at a 1/100 dilution (red) or a rabbit IgG negative control (green).

Western blot - Anti-CACNA1F antibody [EPR11822] - BSA and Azide free (AB249680)
  • WB

Supplier Data

Western blot - Anti-CACNA1F antibody [EPR11822] - BSA and Azide free (AB249680)

This data was developed using ab171968, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CACNA1F antibody [EPR11822] (<a href='/en-us/products/primary-antibodies/cacna1f-antibody-epr11822-ab171968'>ab171968</a>) at 1/1000 dilution

Lane 1:

A673 cell lysate at 10 µg

Lane 2:

Y79 cell lysate at 10 µg

Secondary

All lanes:

Standard HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 219 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11822

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab249680 is the carrier-free version of ab171968.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CACNA1F gene encodes the alpha-1F subunit of a voltage-dependent L-type calcium channel. This protein also known as Cav1.4 has a mass of approximately 230 kDa. It facilitates the influx of calcium ions into the cells in response to changes in electric potential across the cell membrane. The protein is expressed mainly in the retina where it plays an important role in the function of photoreceptor cells.
Biological function summary

The CACNA1F protein influences neurotransmitter release by regulating calcium entry which is essential for visual processing. It functions as a part of a larger voltage-gated calcium channel complex where its role is to fine-tune calcium signals. This precise control is necessary for the proper response to light stimuli in the eye allowing effective signal transmission to the visual pathways.

Pathways

The CACNA1F protein integrates into the calcium signaling pathway important for neuronal communication and sensory processes. It is also linked to the phototransduction cascade in the retina. Within these pathways CACNA1F interacts with related proteins like beta subunits that modulate the activity of the L-type calcium channels impacting overall visual function.

Mutations in the CACNA1F gene are associated with X-linked congenital stationary night blindness and other retinal disorders. These conditions can lead to an impaired vision due to the disrupted calcium flow in photoreceptors. Additionally CACNA1F shows a relationship with other calcium channel proteins like CACNA1S potentially influencing disorder phenotypes through altered ionic homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform 1. Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1F gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, and by benzothiazepines. Activates at more negative voltages and does not undergo calcium-dependent inactivation (CDI), due to incoming calcium ions, during depolarization.. Isoform 4. Voltage-dependent L-type calcium channel activates at more hyperpolarized voltages and exhibits a robust calcium-dependent inactivation (CDI), due to incoming calcium ions, during depolarizations.. Isoform 5. Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death.. Isoform 6. Voltage-dependent L-type calcium channel activates at more hyperpolarized voltages and exhibits a robust calcium-dependent inactivation (CDI), due to incoming calcium ions, during depolarizations.
See full target information CACNA1F
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2021:8379962 PubMed34630853

2021

circ_0023461 Silencing Protects Cardiomyocytes from Hypoxia-Induced Dysfunction through Targeting miR-370-3p/PDE4D Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Ren,Buying Li,Liqing Jiang,Zhiheng Liu,Fan Wu,Yi Zhang,Jincheng Liu,Weixun Duan
View all publications
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