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AB135974

Anti-CACNA1H antibody

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(6 Publications)

Rabbit Polyclonal CACNA1H antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human CACNA1H.

View Alternative Names

Voltage-dependent T-type calcium channel subunit alpha-1H, Low-voltage-activated calcium channel alpha1 3.2 subunit, Voltage-gated calcium channel subunit alpha Cav3.2, CACNA1H

1 Images
Western blot - Anti-CACNA1H antibody (AB135974)
  • WB

Unknown

Western blot - Anti-CACNA1H antibody (AB135974)

All lanes:

Western blot - Anti-CACNA1H antibody (ab135974) at 1/500 dilution

All lanes:

HeLa cell lysate at 30 µg

Predicted band size: 259 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human CACNA1H. The exact immunogen used to generate this antibody is proprietary information.

O95180

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab135974 was affinity-purified from rabbit antiserum by immunogenic peptide affinity-chromatography.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CACNA1H also known as Cav3.2 or T-type calcium channel alpha-1H subunit functions as a voltage-dependent calcium channel. The protein plays a role in the influx of calcium ions into cells. It has a molecular mass of approximately 260.7 kDa. CACNA1H expresses most abundantly in the central nervous system although you can also find it in the heart and various other tissues. This channel is part of the larger Cav3 family of T-type calcium channels which includes other subunits like Cav3.1 and Cav3.3.
Biological function summary

This calcium channel regulates numerous cellular processes through its role in calcium signaling. It does not form part of a complex but still interacts closely with various signaling molecules to influence activities such as neurotransmitter release and muscle contraction. By modulating membrane potentials and synaptic transmission CACNA1H plays a significant direct role in neuronal excitability and rhythmic activities.

Pathways

CACNA1H interacts within the calcium signaling and neurotransmission pathways. In these pathways it regulates calcium ion homeostasis and neuronal excitability working alongside other channels like L-type and N-type calcium channels. CACNA1H affects the excitation-contraction coupling critical for smooth muscle function interlinking with proteins such as calmodulin that respond to changes in calcium ion concentration.

CACNA1H has been linked to epilepsy and neuropathic pain. In epilepsy mutations in CACNA1H can disrupt normal calcium flow affecting neuronal firing patterns and contributing to seizures. Its interaction with other ion channels like SCN1A further influences epileptic manifestations. Mutations may also contribute to neuropathic pain by altering pain threshold and response through its role in pain pathway modifications.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Voltage-sensitive calcium channel that gives rise to T-type calcium currents. T-type calcium channels belong to the 'low-voltage activated (LVA)' group. A particularity of this type of channel is an opening at quite negative potentials, and a voltage-dependent inactivation (PubMed : 27149520, PubMed : 9670923, PubMed : 9930755). T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and support calcium signaling in secretory cells and vascular smooth muscle (Probable). They may also be involved in the modulation of firing patterns of neurons (PubMed : 15048902). In the adrenal zona glomerulosa, participates in the signaling pathway leading to aldosterone production in response to either AGT/angiotensin II, or hyperkalemia (PubMed : 25907736, PubMed : 27729216).
See full target information CACNA1H
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Neural regeneration research 19:2480-2487 PubMed38526284

2024

Cav3.2 channel regulates cerebral ischemia/reperfusion injury: a promising target for intervention.

Applications

Unspecified application

Species

Unspecified reactive species

Feibiao Dai,Chengyun Hu,Xue Li,Zhetao Zhang,Hongtao Wang,Wanjun Zhou,Jiawu Wang,Qingtian Geng,Yongfei Dong,Chaoliang Tang

Environmental toxicology 39:357-366 PubMed37755144

2023

MiRNA-26a-5p inhibits preterm labor initiation by targeting and regulating TRPC3 ion channel protein expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Chen,Tong Liu,Hong Cui,Quan Na,Sishi Liu

Translational psychiatry 12:234 PubMed35668055

2022

Rare CACNA1H and RELN variants interact through mTORC1 pathway in oligogenic autism spectrum disorder.

Applications

Unspecified application

Species

Unspecified reactive species

André Luíz Teles E Silva,Talita Glaser,Karina Griesi-Oliveira,Juliana Corrêa-Velloso,Jaqueline Yu Ting Wang,Gabriele da Silva Campos,Henning Ulrich,Andrea Balan,Mehdi Zarrei,Edward J Higginbotham,Stephen W Scherer,Maria Rita Passos-Bueno,Andrea Laurato Sertié

Nature cardiovascular research 1:577-591 PubMed39195867

2022

Piezo1 is the cardiac mechanosensor that initiates the cardiomyocyte hypertrophic response to pressure overload in adult mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ze-Yan Yu,Hutao Gong,Scott Kesteven,Yang Guo,Jianxin Wu,Jinyuan Vero Li,Delfine Cheng,Zijing Zhou,Siiri E Iismaa,Xenia Kaidonis,Robert M Graham,Charles D Cox,Michael P Feneley,Boris Martinac

Frontiers in neurology 12:741054 PubMed34917013

2021

Change in Cav3.2 T-Type Calcium Channel Induced by Varicella-Zoster Virus Participates in the Maintenance of Herpetic Neuralgia.

Applications

Unspecified application

Species

Unspecified reactive species

Rongzhen Li,Mingxi Ou,Shaomin Yang,Jiabin Huang,Jiamin Chen,Donglin Xiong,Lizu Xiao,Songbin Wu

Acta pharmacologica Sinica 38:1663-1672 PubMed28713161

2017

Functional expression of the Ca signaling machinery in human embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Jun Huang,Yi-Jie Wang,Min Zhang,Peng Zhang,He Liang,Hua-Jun Bai,Xiu-Jian Yu,Huang-Tian Yang
View all publications
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Product promise

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