AB84815

Anti-CACNA1H antibody [N55/10]

Name: Anti-CACNA1H antibody [N55/10] (ab84815) | Abcam
Brand: Abcam Limited
SKU: AB84815

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(2 Publications)

Mouse Monoclonal CACNA1H antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Rat, Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Fusion protein corresponding to aa 1000-1300 of CACNA1H, fused to CACNA1H.

View Alternative Names

Voltage-dependent T-type calcium channel subunit alpha-1H, Low-voltage-activated calcium channel alpha1 3.2 subunit, Voltage-gated calcium channel subunit alpha Cav3.2, CACNA1H

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1H antibody [N55/10] (AB84815)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1H antibody [N55/10] (AB84815)

Immunohistochemical analysis (Formalin/PFA-fixed paraffin-embedded sections) of human hippocampus tissue labelling CACNA1H with ab84815 at 1 : 1000 dilution, followed by secondary antibody Goat anti-mouse (green) at 1 : 50 for 1 hour at RT.

Immunohistochemistry (Frozen sections) - Anti-CACNA1H antibody [N55/10] (AB84815)

IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-CACNA1H antibody [N55/10] (AB84815)

Immunohistochemical analysis of frozen Human brain tissue sections staining CACNA1H. Samples were fixed with 10% Formalin solution and incubated with primary antibody at 1 : 1000 dilution for 1 hour at room temperature. A biotinylated goat anti-mouse was used as the secondary antibody. Mayer Hematoxylin was used as a nuclear stain.

Supplier Data

Western blot - Anti-CACNA1H antibody [N55/10] (AB84815)

Blocking and dilution buffer : 2% Skim Milk + 2% BSA in TBST.

All lanes:

Western blot - Anti-CACNA1H antibody [N55/10] (ab84815) at 1/1000 dilution

All lanes:

Rat brain membrane lysate

Secondary

All lanes:

Anti-Mouse HRP at 1/4000 dilution

Predicted band size: 261 kDa

Observed band size: 261 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

N55/10

Isotype

IgG1

Carrier free

Reacts with

Human, Mouse, Rat

Applications

IHC-Fr, WB, IHC-P

applications

Immunogen

Fusion protein corresponding to aa 1000-1300 of CACNA1H, fused to CACNA1H. The exact immunogen used to generate this antibody is proprietary information.

O95180

Specificity

No cross-reactivity against Cav1.3 and Cav3.1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/1000", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "1/1000", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

The clone number has been updated from S55-10 to N55/10, both clone numbers name the same antibody clone.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CACNA1H also known as Cav3.2 or T-type calcium channel alpha-1H subunit functions as a voltage-dependent calcium channel. The protein plays a role in the influx of calcium ions into cells. It has a molecular mass of approximately 260.7 kDa. CACNA1H expresses most abundantly in the central nervous system although you can also find it in the heart and various other tissues. This channel is part of the larger Cav3 family of T-type calcium channels which includes other subunits like Cav3.1 and Cav3.3.

Biological function summary

This calcium channel regulates numerous cellular processes through its role in calcium signaling. It does not form part of a complex but still interacts closely with various signaling molecules to influence activities such as neurotransmitter release and muscle contraction. By modulating membrane potentials and synaptic transmission CACNA1H plays a significant direct role in neuronal excitability and rhythmic activities.

Pathways

CACNA1H interacts within the calcium signaling and neurotransmission pathways. In these pathways it regulates calcium ion homeostasis and neuronal excitability working alongside other channels like L-type and N-type calcium channels. CACNA1H affects the excitation-contraction coupling critical for smooth muscle function interlinking with proteins such as calmodulin that respond to changes in calcium ion concentration.

CACNA1H has been linked to epilepsy and neuropathic pain. In epilepsy mutations in CACNA1H can disrupt normal calcium flow affecting neuronal firing patterns and contributing to seizures. Its interaction with other ion channels like SCN1A further influences epileptic manifestations. Mutations may also contribute to neuropathic pain by altering pain threshold and response through its role in pain pathway modifications.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Voltage-sensitive calcium channel that gives rise to T-type calcium currents. T-type calcium channels belong to the 'low-voltage activated (LVA)' group. A particularity of this type of channel is an opening at quite negative potentials, and a voltage-dependent inactivation (PubMed : 27149520, PubMed : 9670923, PubMed : 9930755). T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and support calcium signaling in secretory cells and vascular smooth muscle (Probable). They may also be involved in the modulation of firing patterns of neurons (PubMed : 15048902). In the adrenal zona glomerulosa, participates in the signaling pathway leading to aldosterone production in response to either AGT/angiotensin II, or hyperkalemia (PubMed : 25907736, PubMed : 27729216).

See full target information CACNA1H

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cephalalgia : an international journal of headache 33:123-9 PubMed23111027

2012

CACNA1H antibodies associated with headache with neurological deficits and cerebrospinal fluid lymphocytosis (HaNDL).

Applications

Species

Human

Murat Kürtüncü,Dilaver Kaya,Luigi Zuliani,Ece Erdağ,Sema Içöz,Elif Uğurel,Filiz Cavuş,Neşe Ayşit,Omer Birişik,Angela Vincent,Mefkure Eraksoy,Burçak Vural,Gülşen Akman-Demir,Erdem Tüzün

International journal of oncology 41:267-75 PubMed22469755

2012

T-type voltage-activated calcium channel Cav3.1, but not Cav3.2, is involved in the inhibition of proliferation and apoptosis in MCF-7 human breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tsuyako Ohkubo,Jun Yamazaki

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CACNA1H antibody [N55/10]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1H antibody [N55/10] (AB84815)

Immunohistochemistry (Frozen sections) - Anti-CACNA1H antibody [N55/10] (AB84815)

Western blot - Anti-CACNA1H antibody [N55/10] (AB84815)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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