AB203662

Anti-CACNA1S antibody

Name: Anti-CACNA1S antibody (ab203662) | Abcam
Brand: Abcam Limited
SKU: AB203662

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(2 Publications)

Rabbit Polyclonal CACNA1S antibody. Suitable for IHC-P and reacts with Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CACNA1S aa 950-1050 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CACH1, CACN1, CACNL1A3, CACNA1S, Voltage-dependent L-type calcium channel subunit alpha-1S, Voltage-gated calcium channel subunit alpha Cav1.1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1S antibody (AB203662)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1S antibody (AB203662)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat skeletal muscle tissue labeling CACNA1S with ab203662 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1S antibody (AB203662)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse embryo tissue labeling CACNA1S with ab203662 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human CACNA1S aa 950-1050 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q13698

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>(or 1/50 - 1/200 using a fluorescent secondary antibody).</p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>(or 1/50 - 1/200 using a fluorescent secondary antibody).</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CACNA1S also known as Cav1.1 is a voltage-dependent calcium channel subunit alpha-1S with a molecular mass of approximately 212 kDa. This protein is expressed prominently in skeletal muscle tissues. It is essential for mediating calcium ion influx across the cell membrane necessary for muscle contraction. CACNA1S is characterized by its role in forming L-type calcium channels which contribute to the excitation-contraction coupling process in muscle cells.

Biological function summary

CACNA1S functions as an important component in the skeletal muscle contraction mechanism. It is part of a large protein complex including other subunits like beta gamma and delta which together form the functional calcium channel. This channel through voltage-sensing and conformational changes in CACNA1S allows the regulated flow of calcium ions into muscle cells triggering biochemical cascades that result in contraction.

Pathways

CACNA1S plays a critical role in the excitation-contraction coupling and calcium signaling pathways. It interacts with proteins such as the ryanodine receptor (RyR1) to ensure effective calcium release from the sarcoplasmic reticulum facilitating proper skeletal muscle function. Additionally it is associated with calmodulin a calcium-binding messenger protein that modulates channel activity and plays a role in muscle fiber function.

Alterations in CACNA1S are linked to conditions like hypokalemic periodic paralysis and malignant hyperthermia. In hypokalemic periodic paralysis CACNA1S mutations disrupt normal calcium channel function causing episodes of muscle weakness. Meanwhile its association with malignant hyperthermia reveals connections to the ryanodine receptor where abnormal calcium release during anesthesia can lead to severe reactions. Understanding CACNA1S interactions therefore provides insights into these muscle-related conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Pore-forming, alpha-1S subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents in skeletal muscle. Calcium channels containing the alpha-1S subunit play an important role in excitation-contraction coupling in skeletal muscle via their interaction with RYR1, which triggers Ca(2+) release from the sarcoplasmic reticulum and ultimately results in muscle contraction. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group.

See full target information CACNA1S

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Disease models & mechanisms 15: PubMed35293586

2022

Characterization of a novel zebrafish model of SPEG-related centronuclear myopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Karla G Espinosa,Salma Geissah,Linda Groom,Jonathan Volpatti,Ian C Scott,Robert T Dirksen,Mo Zhao,James J Dowling

Inflammation research : official journal of the European Histamine Research Society ... [et al.] 68:945-955 PubMed31444514

2019

Anti-angiogenic and anti-inflammatory effects of CD200-CD200R1 axis in oxygen-induced retinopathy mice model.

Applications

Unspecified application

Species

Unspecified reactive species

Yaguang Hu,Ting Wei,Shan Gao,Qiaochu Cheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CACNA1S antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1S antibody (AB203662)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1S antibody (AB203662)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise