Rabbit Polyclonal CACNA1S antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CACNA1S aa 500-900.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CACNA1S aa 500-900. The exact immunogen used to generate this antibody is proprietary information. Database link Q13698
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cat | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Antigen retrieval is not essential but may optimise staining. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Cat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Cat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Pore-forming, alpha-1S subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents in skeletal muscle. Calcium channels containing the alpha-1S subunit play an important role in excitation-contraction coupling in skeletal muscle via their interaction with RYR1, which triggers Ca(2+) release from the sarcoplasmic reticulum and ultimately results in muscle contraction. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group.
Alternative names
CACH1, CACN1, CACNL1A3, CACNA1S, Voltage-dependent L-type calcium channel subunit alpha-1S, Voltage-gated calcium channel subunit alpha Cav1.1
Recommended products
Rabbit Polyclonal CACNA1S antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CACNA1S aa 500-900.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CACNA1S aa 500-900. The exact immunogen used to generate this antibody is proprietary information. Database link Q13698
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CACNA1S also known as Cav1.1 is a voltage-dependent calcium channel subunit alpha-1S with a molecular mass of approximately 212 kDa. This protein is expressed prominently in skeletal muscle tissues. It is essential for mediating calcium ion influx across the cell membrane necessary for muscle contraction. CACNA1S is characterized by its role in forming L-type calcium channels which contribute to the excitation-contraction coupling process in muscle cells.
- Biological function summary
CACNA1S functions as an important component in the skeletal muscle contraction mechanism. It is part of a large protein complex including other subunits like beta gamma and delta which together form the functional calcium channel. This channel through voltage-sensing and conformational changes in CACNA1S allows the regulated flow of calcium ions into muscle cells triggering biochemical cascades that result in contraction.
- Pathways
CACNA1S plays a critical role in the excitation-contraction coupling and calcium signaling pathways. It interacts with proteins such as the ryanodine receptor (RyR1) to ensure effective calcium release from the sarcoplasmic reticulum facilitating proper skeletal muscle function. Additionally it is associated with calmodulin a calcium-binding messenger protein that modulates channel activity and plays a role in muscle fiber function.
- Associated diseases and disorders
Alterations in CACNA1S are linked to conditions like hypokalemic periodic paralysis and malignant hyperthermia. In hypokalemic periodic paralysis CACNA1S mutations disrupt normal calcium channel function causing episodes of muscle weakness. Meanwhile its association with malignant hyperthermia reveals connections to the ryanodine receptor where abnormal calcium release during anesthesia can lead to severe reactions. Understanding CACNA1S interactions therefore provides insights into these muscle-related conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-CACNA1S antibody (ab96413)
5% SDS PAGE
All lanes: Western blot - Anti-CACNA1S antibody (ab96413) at 1/1000 dilution
All lanes: 293T whole cell lysate at 30 µg
Predicted band size: 212 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1S antibody (ab96413)
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ab96413 at 1:100 dilution.
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