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AB249754

Anti-CACNA2D2 antibody [EPR12343] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal CACNA2D2 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

KIAA0558, CACNA2D2, Voltage-dependent calcium channel subunit alpha-2/delta-2, Voltage-gated calcium channel subunit alpha-2/delta-2

3 Images
Immunoprecipitation - Anti-CACNA2D2 antibody [EPR12343] - BSA and Azide free (AB249754)
  • IP

Supplier Data

Immunoprecipitation - Anti-CACNA2D2 antibody [EPR12343] - BSA and Azide free (AB249754)

This data was developed using ab173293, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from fetal brain lysate using ab173293.

All lanes:

Immunoprecipitation - Anti-CACNA2D2 antibody [EPR12343] (<a href='/en-us/products/primary-antibodies/cacna2d2-antibody-epr12343-ab173293'>ab173293</a>)

Predicted band size: 130 kDa

false

Western blot - Anti-CACNA2D2 antibody [EPR12343] - BSA and Azide free (AB249754)
  • WB

Lab

Western blot - Anti-CACNA2D2 antibody [EPR12343] - BSA and Azide free (AB249754)

This data was developed using ab173293, the same antibody clone in a different buffer formulation.

Lane 1 : ild-type HAP1 cell lysate (20 μg)

Lane 2 : ACNA2D2 knockout HAP1 cell lysate (20 μg)

Lane 3 HeLa cell lysate (20 μg)

Lane 4 Human brain tissue lysate (20 μg)

Lanes 1 - 4 : erged signal (red and green). Green - ab173293 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab173293 was shown to specifically react with CACNA2D2 when CACNA2D2 knockout samples were used. Wild-type and CACNA2D2 knockout samples were subjected to SDS-PAGE. ab173293 and ab8245 (loading control to GAPDH) were diluted at 1/5000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CACNA2D2 antibody [EPR12343] (<a href='/en-us/products/primary-antibodies/cacna2d2-antibody-epr12343-ab173293'>ab173293</a>)

Predicted band size: 130 kDa

false

Western blot - Anti-CACNA2D2 antibody [EPR12343] - BSA and Azide free (AB249754)
  • WB

Supplier Data

Western blot - Anti-CACNA2D2 antibody [EPR12343] - BSA and Azide free (AB249754)

This data was developed using ab173293, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CACNA2D2 antibody [EPR12343] (<a href='/en-us/products/primary-antibodies/cacna2d2-antibody-epr12343-ab173293'>ab173293</a>) at 1/1000 dilution

Lane 1:

Fetal lung lysate at 10 µg

Lane 2:

Fetal brain lysate at 10 µg

Lane 3:

Human cerebellum lysate at 10 µg

Predicted band size: 130 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12343

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab249754 is the carrier-free version of ab173293.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CACNA2D2 also known as calcium channel voltage-dependent alpha 2/delta subunit 2 is a protein involved in the function of voltage-gated calcium channels. This protein acts as an auxiliary subunit and plays a role in the regulation of the channel's currents. CACNA2D2 has a mass of approximately 129 kDa and is typically expressed in various tissues including the brain and heart. It shows significant involvement in neurons playing a role in neurotransmitter release due to its modulation of calcium entry into cells.
Biological function summary

CASNA2D2 is important for the function of nervous and cardiac systems. It forms part of a larger protein complex that includes alpha-1 beta and gamma subunits which all work together to influence calcium influx in response to membrane depolarization. This calcium entry is important for processes such as synaptic transmission and muscle contraction. CACNA2D2's modulation of calcium channels hence affects the excitability of neurons and the contractility of cardiac muscle cells impacting both neuronal signaling and heart function.

Pathways

The presence of CACNA2D2 influences the calcium signaling pathway and the cardiac conduction pathway. This protein modifies cellular processes by regulating the calcium ion concentration inside cells which can trigger various signaling cascades. It interacts closely with other proteins in these pathways like the L-type calcium channels which mediate significant increases in intracellular calcium levels. Such interactions further highlight its role in normal physiological responses to electrical stimuli in cells.

Alterations in CACNA2D2 expression or function can lead to epilepsy and cardiac arrhythmias. Changes in this protein's behavior can disrupt normal calcium homeostasis leading to altered neuronal firing patterns and impaired cardiac rhythm. Its relationship with epilepsy highlights its role as a potential target for anticonvulsant drugs aiming to stabilize neuronal firing. Similarly it connects with other proteins like CACNA1C in the context of cardiac diseases linking these proteins in the pathological manifestation of arrhythmias.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Acts as a regulatory subunit for P/Q-type calcium channel (CACNA1A), N-type (CACNA1B), L-type (CACNA1C OR CACNA1D) and possibly T-type (CACNA1G) (PubMed : 15111129, PubMed : 23339110). Overexpression induces apoptosis.
See full target information CACNA2D2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2021:8379962 PubMed34630853

2021

circ_0023461 Silencing Protects Cardiomyocytes from Hypoxia-Induced Dysfunction through Targeting miR-370-3p/PDE4D Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Ren,Buying Li,Liqing Jiang,Zhiheng Liu,Fan Wu,Yi Zhang,Jincheng Liu,Weixun Duan
View all publications
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Product promise

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