AB155179

Anti-CACNB1/CAB1 antibody

Name: Anti-CACNB1/CAB1 antibody (ab155179) | Abcam
Brand: Abcam Limited
SKU: AB155179

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(2 Publications)

Rabbit Polyclonal CACNB1/CAB1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CACNB1 aa 300 to C-terminus.

View Alternative Names

CACNLB1, CACNB1, Voltage-dependent L-type calcium channel subunit beta-1, CAB1, Calcium channel voltage-dependent subunit beta 1

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-CACNB1/CAB1 antibody (AB155179)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CACNB1/CAB1 antibody (AB155179)

Immunofluorescence analysis of methanol-fixed HeLa cells labeling CACNB1/ CAB1 with ab155179 at 1/200 dilution (green). Lower image shows cells co-stained with Hoechst 33342.

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Western blot - Anti-CACNB1/CAB1 antibody (AB155179)

7.5% SDS PAGE

All lanes:

Western blot - Anti-CACNB1/CAB1 antibody (ab155179) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

HepG2 whole cell lysate at 30 µg

Lane 3:

Raji whole cell lysate at 30 µg

Predicted band size: 66 kDa

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Supplier Data

Western blot - Anti-CACNB1/CAB1 antibody (AB155179)

Samples were separated by 7.5% SDS PAGE.

All lanes:

Western blot - Anti-CACNB1/CAB1 antibody (ab155179) at 1/1000 dilution

All lanes:

Rat brain tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 66 kDa

false

Supplier Data

Western blot - Anti-CACNB1/CAB1 antibody (AB155179)

Samples were separated by 7.5% SDS PAGE.

All lanes:

Western blot - Anti-CACNB1/CAB1 antibody (ab155179) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 50 µg

Lane 2:

Mouse lung tissue lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 66 kDa

false

Unknown

Western blot - Anti-CACNB1/CAB1 antibody (AB155179)

7.5% SDS PAGE

All lanes:

Western blot - Anti-CACNB1/CAB1 antibody (ab155179) at 1/1000 dilution

All lanes:

Mouse brain lysate at 50 µg

Predicted band size: 66 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human CACNB1 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q02641

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CACNB1 also known as CAB1 is a protein that forms a part of the voltage-dependent L-type calcium channel complex. It is one of the auxiliary beta subunits and plays an important role in regulating the activity of the channel by stabilizing the alpha 1 subunit's structure. CACNB1 has a molecular weight of approximately 65 kDa. Its expression takes place in various tissues including the heart skeletal muscle and brain which indicates its importance in excitable tissues.

Biological function summary

Several processes depend on CACNB1's function. This protein contributes significantly to modulating calcium ion influx into cells a fundamental function for cellular activities such as muscle contraction neurotransmitter release and gene expression. CACNB1 exists as part of the calcium channel complex and interacts directly with the alpha 1 subunit which influences channel properties and cell surface expression.

Pathways

CACNB1 is integral to the calcium signaling pathway and cardiac muscle contraction pathway. It directly interacts with the alpha 1 subunit of the L-type calcium channels facilitating calcium ion entry that is necessary for proper signal transduction. In these pathways other proteins such as CACNA1C also play a significant role by forming part of the channel complex that mediates these essential physiological effects.

CACNB1 expression relates to conditions like cardiac arrhythmia and skeletal muscle disorders. Changes in its expression or function can disrupt normal calcium ion balance and lead to these diseases. Interactions with proteins like CACNA1C which also plays a role in channel function are of particular interest as alterations in these proteins are often seen in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulatory subunit of L-type calcium channels (PubMed : 1309651, PubMed : 15615847, PubMed : 8107964). Regulates the activity of L-type calcium channels that contain CACNA1A as pore-forming subunit (By similarity). Regulates the activity of L-type calcium channels that contain CACNA1C as pore-forming subunit and increases the presence of the channel complex at the cell membrane (PubMed : 15615847). Required for functional expression L-type calcium channels that contain CACNA1D as pore-forming subunit (PubMed : 1309651). Regulates the activity of L-type calcium channels that contain CACNA1B as pore-forming subunit (PubMed : 8107964).

See full target information CACNB1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of physiology 602:6729-6744 PubMed39612361

2024

Rad-mediated inhibition of Ca1.2 channel activity contributes to uterine artery haemodynamic adaptation to pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang-Qun Hu,Rui Song,Chiranjib Dasgupta,Taiming Liu,Meijuan Zhang,Stephen Twum-Barimah,Arlin B Blood,Lubo Zhang

Obesity (Silver Spring, Md.) 30:153-164 PubMed34825496

2021

Combining a β3 adrenergic receptor agonist with alpha-lipoic acid reduces inflammation in male mice with diet-induced obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Zahraa Abdul Sater,Cheryl Cero,Anne E Pierce,Hannah J Lea,Houssein Abdul Sater,Kenneth Y Zhu,Naili Liu,Yinyan Ma,Oksana Gavrilova,Aaron M Cypess

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CACNB1/CAB1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-CACNB1/CAB1 antibody (AB155179)

Western blot - Anti-CACNB1/CAB1 antibody (AB155179)

Western blot - Anti-CACNB1/CAB1 antibody (AB155179)

Western blot - Anti-CACNB1/CAB1 antibody (AB155179)

Western blot - Anti-CACNB1/CAB1 antibody (AB155179)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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