Anti-CACNB2 antibody [N8B/1]

4 product images

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  Flow Cytometry - Anti-CACNB2 antibody [N8B/1] (ab93606)

  Overlay histogram showing SH-SY5Y cells stained with ab93606 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93606, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  
  

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNB2 antibody [N8B/1] (ab93606)

  IHC image of ab93606 staining in Human Heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93606, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  
  

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  Immunocytochemistry/ Immunofluorescence - Anti-CACNB2 antibody [N8B/1] (ab93606)

  Cold 100% methanol-fixed HaCaT (human keratinocyte cell line) cells stained for CACNB2 (green) using ab93606 at 1/100 dilution in ICC/IF.
  

  Secondary Antibody: FITC Goat Anti-Mouse at 1/50 dilution for 1 hour at room temperature.

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  Immunocytochemistry/ Immunofluorescence - Anti-CACNB2 antibody [N8B/1] (ab93606)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed Neuroblastoma cells (SH-SY5Y) labeling CACNB2 with ab93606 at 1/50 dilution. Secondary antibody: AlexaFluor® 488 at 1/1000 dilution. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav beta 2 Antibody (D) Composite.