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AB93606

Anti-CACNB2 antibody [N8B/1]

5

(1 Review)

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(3 Publications)

Mouse Monoclonal CACB2 antibody. Suitable for IHC-P, IP, Flow Cyt, WB, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

AW060387, CAB2, CACB2_HUMAN, CACNB2, CACNLB2, CAVB2, Ca(V) beta 2, Calcium channel voltage-dependent subunit beta 2, Calcium channel, voltage dependent, beta 2 subunit, Cavbeta2, Cchb2, FLJ23743, Lambert Eaton Myasthenic syndrome antigen, Lambert-Eaton myasthenic syndrome antigen B, MGC129334, MGC129335, MYSB, Myasthenic (Lambert Eaton) syndrome antigen B, Myasthenic syndrome antigen B

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNB2 antibody [N8B/1] (AB93606)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNB2 antibody [N8B/1] (AB93606)

IHC image of ab93606 staining in Human Heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93606, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunocytochemistry/ Immunofluorescence - Anti-CACNB2 antibody [N8B/1] (AB93606)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CACNB2 antibody [N8B/1] (AB93606)

Cold 100% methanol-fixed HaCaT (human keratinocyte cell line) cells stained for CACNB2 (green) using ab93606 at 1/100 dilution in ICC/IF.

Secondary Antibody : FITC Goat Anti-Mouse at 1/50 dilution for 1 hour at room temperature.

Flow Cytometry - Anti-CACNB2 antibody [N8B/1] (AB93606)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CACNB2 antibody [N8B/1] (AB93606)

Overlay histogram showing SH-SY5Y cells stained with ab93606 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93606, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Immunocytochemistry/ Immunofluorescence - Anti-CACNB2 antibody [N8B/1] (AB93606)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CACNB2 antibody [N8B/1] (AB93606)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed Neuroblastoma cells (SH-SY5Y) labeling CACNB2 with ab93606 at 1/50 dilution. Secondary antibody : AlexaFluor® 488 at 1/1000 dilution. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav beta 2 Antibody (D) Composite.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

N8B/1

Isotype

IgG1

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, WB, IHC-P, IHC-Fr, Flow Cyt, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

No cross reactivity against CAVB1, CAVB3 and CAVB4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Antigen retrieval is not essential but may optimise staining.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-10 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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Product details

The clone number has been updated from S8b-1 to N8B/1, both clone numbers name the same antibody clone.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CACNB2 also known as the calcium channel voltage-dependent beta 2 subunit is a part of the high voltage-gated calcium channel complex. The protein encoded by CACNB2 gene has a mass of approximately 66 kDa. It exists within many tissues including cardiac tissue skeletal muscle and the brain. This protein plays a role in modulating the activity of the channel by interacting with both alpha and gamma subunits influencing calcium ion influx.
Biological function summary

CACNB2 participates in controlling calcium channel activity which is significant for various cellular processes. It forms a part of the channel complex that helps stabilize the arrangement and activity of the pore-forming alpha subunit. Through this CACNB2 affects processes such as muscle contraction neurotransmitter release and gene expression. Interactions of CACNB2 subunits help regulate the amplitude and kinetics of calcium currents important for cellular communication.

Pathways

CACNB2 regulates the L-type calcium channel pathway integral to the excitation-contraction coupling in cardiac and muscle cells. It associates with other calcium channel subunits like CACNA1C modulating calcium channel functionality. In the MAPK signaling pathway CACNB2 indirectly influences cellular responses to various stimuli. This protein provides essential inputs to these pathways by affecting calcium ion flow into cells which is critical for their activation and operation.

CACNB2 exhibits a connection to conditions such as cardiac arrhythmias and epilepsy. Its role in calcium channel functioning relates to arrhythmogenic activities in cardiac cells when abnormalities in ion flow occur. There is also evidence linking CACNB2 to epilepsy where disrupted calcium flow affects neuronal firing patterns. Other related proteins like CACNA1C also play a part in these disorders highlighting the complex interactions between voltage-gated calcium channel components in disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Voltage-dependent L-type calcium channel subunit beta-2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:596484 PubMed33777927

2021

Immune Profiling Reveals Molecular Classification and Characteristic in Urothelial Bladder Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Li Yang,Aitian Li,Fengsen Liu,Qitai Zhao,Shaofei Ji,Wen Zhu,Weina Yu,Ru Zhang,Yaqing Liu,Wencai Li,Yi Zhang

PloS one 8:e73224 PubMed23951345

2013

Identification of new dystroglycan complexes in skeletal muscle.

Applications

Unspecified application

Species

Mouse

Eric K Johnson,Bin Li,Jung Hae Yoon,Kevin M Flanigan,Paul T Martin,James Ervasti,Federica Montanaro

The Journal of biological chemistry 287:32588-97 PubMed22851179

2012

A short polybasic segment between the two conserved domains of the β2a-subunit modulates the rate of inactivation of R-type calcium channel.

Applications

WB

Species

Unspecified reactive species

Erick Miranda-Laferte,Silke Schmidt,Antonella C Jara,Alan Neely,Patricia Hidalgo
View all publications
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Product promise

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