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AB171972

Anti-CacyBP antibody [EPR12374]

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(5 Publications)

Rabbit Recombinant Monoclonal CacyBP antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

View Alternative Names

S100A6BP, SIP, PNAS-107, CACYBP, Calcyclin-binding protein, CacyBP, hCacyBP, S100A6-binding protein, Siah-interacting protein

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CacyBP antibody [EPR12374] (AB171972)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CacyBP antibody [EPR12374] (AB171972)

Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling CacyBP with ab171972 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CacyBP antibody [EPR12374] (AB171972)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CacyBP antibody [EPR12374] (AB171972)

Immunohistochemical analysis of paraffin-embedded Human gliocytoma tissue labeling CacyBP with ab171972 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-CacyBP antibody [EPR12374] (AB171972)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CacyBP antibody [EPR12374] (AB171972)

Immunofluorescent analysis of MCF7 cells labeling CacyBP with ab171972 at 1/100 dilution. DAPI nuclear staining (blue).

Flow Cytometry (Intracellular) - Anti-CacyBP antibody [EPR12374] (AB171972)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CacyBP antibody [EPR12374] (AB171972)

Intracellular flow cytometric analysis of permeabilized 293T cells labeling CacyBPwith ab171972 at 1/10 dilution (red) orRabbit IgG (negative) (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CacyBP antibody [EPR12374] (AB171972)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CacyBP antibody [EPR12374] (AB171972)

Immunohistochemical analysis of paraffin-embedded Human Kidney tissue labeling CacyBP with ab171972 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-CacyBP antibody [EPR12374] (AB171972)
  • IP

Supplier Data

Immunoprecipitation - Anti-CacyBP antibody [EPR12374] (AB171972)

Western blot analysis on immunoprecipitation pellet from 293T cell lysate (lane 1) labeling CacyBP with ab171972 at 1/10 dilution or 1X PBS (lane 2) negative control. HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.

All lanes:

Immunoprecipitation - Anti-CacyBP antibody [EPR12374] (ab171972)

Predicted band size: 26 kDa

false

Western blot - Anti-CacyBP antibody [EPR12374] (AB171972)
  • WB

Supplier Data

Western blot - Anti-CacyBP antibody [EPR12374] (AB171972)

All lanes:

Western blot - Anti-CacyBP antibody [EPR12374] (ab171972) at 1/10000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

PC3 cell lysate at 10 µg

Predicted band size: 26 kDa

false

Western blot - Anti-CacyBP antibody [EPR12374] (AB171972)
  • WB

Lab

Western blot - Anti-CacyBP antibody [EPR12374] (AB171972)

Western blot : Anti-CacyBP antibody [EPR12374] ab171972 staining at 1/10000 dilution, shown in green; Mouse anti alpha Tubulin ab7291 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 30 kDa in Wild-type A549 cell lysates with no signal observed at this size in CACYBP knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-CacyBP antibody [EPR12374] (ab171972) at 1/10000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

CACYBP knockout A549 at 20 µg

Lane 3:

Raji at 20 µg

Lane 4:

PC-3 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 26 kDa

Observed band size: 30 kDa,52 kDa

false

  • Carrier free

    Anti-CacyBP antibody [EPR12374] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12374

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IP, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/10000 - 1/50000", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CacyBP also known as Calcyclin Binding Protein is involved in various cellular processes and interactions. This protein has a molecular mass of about 30 kDa. It is broadly expressed in many tissues including the brain where it plays significant roles. CacyBP interacts with several other proteins impacting cellular structures and functions through complex interactions.
Biological function summary

CacyBP regulates key cellular functions by interacting with signaling pathways and cytoskeletal elements. It can form part of multi-protein complexes influencing the stability and structure of cytoskeletal components. CacyBP also modifies proteins by dephosphorylation impacting processes such as cell division and differentiation. These functions underline its involvement in cellular maintenance and dynamic processes.

Pathways

CacyBP participates in cellular signaling and protein degradation pathways. It plays a role in the ubiquitin-proteasome pathway by mediating the degradation of specific substrate proteins. This protein associates with Siah-1 an E3 ubiquitin-protein ligase emphasizing its function in this pathway. CacyBP is also linked to ERK1/2 signaling pathways interacting with proteins like ERK2 to modulate cell proliferation and differentiation.

CacyBP has connections to cancer and neurodegenerative diseases. In cancer CacyBP influences tumor growth and progression by affecting cell cycle regulation and apoptosis. It interacts with proteins such as p53 which play roles in cell cycle control and tumor suppression. In neurodegenerative diseases like Alzheimer's altered CacyBP expression levels have been observed implicating it in the disorder’s pathology through interactions with tau protein.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1).
See full target information CACYBP
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules & biomedicine 25:1801-1815 PubMed40167359

2025

Silencing CACYBP suppresses lung adenocarcinoma growth via CDK1 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Ge Wen,Shaoqing Niu,Shiqi Mei,Senming Wang

Oncology research 31:317-331 PubMed37305391

2023

Calcyclin-binding protein contributes to cholangiocarcinoma progression by inhibiting ubiquitination of MCM2.

Applications

Unspecified application

Species

Unspecified reactive species

Yusen Zhang,Liping Liu,Biwei Luo,Honggui Tang,Xiaofang Yu,Shiyun Bao

Journal of cancer research and clinical oncology 149:5761-5772 PubMed36576589

2022

CACYBP knockdown inhibits progression of prostate cancer via p53.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Li,Zhili Liu,Luping Ma,Weiqi Yin,Kan Zhang

Redox biology 58:102544 PubMed36427397

2022

CPT1A promotes anoikis resistance in esophageal squamous cell carcinoma via redox homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Tian,Yunxin Lu,Jinfei Lin,Miao Chen,Huijuan Qiu,Wancui Zhu,Haohui Sun,Jinsheng Huang,Han Yang,Wuguo Deng

Cellular and molecular life sciences : CMLS 75:4371-4384 PubMed30032357

2018

Serine protease PRSS55 is crucial for male mouse fertility via affecting sperm migration and sperm-egg binding.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Shang,Chunling Shen,Jianbing Liu,Lingyun Tang,Hongxin Zhang,Yicheng Wang,Wenting Wu,Jun Chi,Hua Zhuang,Jian Fei,Zhugang Wang
View all publications
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