Anti-CacyBP antibody [EPR12374] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CacyBP antibody [EPR12374] - BSA and Azide free (AB249684)

This data was developed using ab171972, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling CacyBP with ab171972 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CacyBP antibody [EPR12374] - BSA and Azide free (AB249684)

This data was developed using ab171972, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human gliocytoma tissue labeling CacyBP with ab171972 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CacyBP antibody [EPR12374] - BSA and Azide free (AB249684)

This data was developed using ab171972, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of MCF7 cells labeling CacyBP with ab171972 at 1/100 dilution. DAPI nuclear staining (blue).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CacyBP antibody [EPR12374] - BSA and Azide free (AB249684)

This data was developed using ab171972, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized 293T cells labeling CacyBP with ab171972 at 1/10 dilution (red) or Rabbit IgG (negative) (green).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CacyBP antibody [EPR12374] - BSA and Azide free (AB249684)

This data was developed using ab171972, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human Kidney tissue labeling CacyBP with ab171972 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-CacyBP antibody [EPR12374] - BSA and Azide free (AB249684)

This data was developed using ab171972, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from 293T cell lysate (lane 1) labeling CacyBP with ab171972 at 1/10 dilution or 1X PBS (lane 2) negative control. HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.

All lanes:

Immunoprecipitation - Anti-CacyBP antibody [EPR12374] (<a href='/en-us/products/primary-antibodies/cacybp-antibody-epr12374-ab171972'>ab171972</a>)

Predicted band size: 26 kDa

false

• WB

Supplier Data

Western blot - Anti-CacyBP antibody [EPR12374] - BSA and Azide free (AB249684)

This data was developed using ab171972, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CacyBP antibody [EPR12374] (<a href='/en-us/products/primary-antibodies/cacybp-antibody-epr12374-ab171972'>ab171972</a>) at 1/10000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

PC3 cell lysate at 10 µg

Predicted band size: 26 kDa

false

• WB

Lab

Western blot - Anti-CacyBP antibody [EPR12374] - BSA and Azide free (AB249684)

This data was developed using ab171972, the same antibody clone in a different buffer formulation.

Western blot : Anti-CacyBP antibody [EPR12374] ab171972 staining at 1/10000 dilution, shown in green; Mouse anti alpha Tubulin ab7291 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 30 kDa in Wild-type A549 cell lysates with no signal observed at this size in CACYBP knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-CacyBP antibody [EPR12374] (<a href='/en-us/products/primary-antibodies/cacybp-antibody-epr12374-ab171972'>ab171972</a>) at 1/10000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

CACYBP knockout A549 at 20 µg

Lane 3:

Raji at 20 µg

Lane 4:

PC-3 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 26 kDa

Observed band size: 30 kDa,52 kDa

false