AB270263

Anti-Cadherin 16 antibody [CDH16/1532R]

Recombinant
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(3 Publications )

Rabbit Recombinant Monoclonal Cadherin 16 antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CDH16.

View Alternative Names

UNQ695/PRO1340, CDH16, Cadherin-16, Kidney-specific cadherin, Ksp-cadherin

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] (AB270263)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] (AB270263)

Formalin-fixed, paraffin-embedded human renal cell carcinoma stained for Cadherin 16 using ab270263 at 2 μg/ml in imunnohistochemical analysis.

Flow Cytometry - Anti-Cadherin 16 antibody [CDH16/1532R] (AB270263)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Cadherin 16 antibody [CDH16/1532R] (AB270263)

Flow cytometric analysis of HEK-293 (Human epithelial cell line from embryonic kidney) cells labeling Cadherin 16 (Blue) using ab270263 at 2μg/10⁶ cells followed by goat anti-rabbit IgG-CF488; isotype control (Red).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] (AB270263)

Formalin-fixed, paraffin-embedded mouse kidney stained for Cadherin 16 using ab270263 at 2 μg/ml in imunnohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] (AB270263)

Formalin-fixed, paraffin-embedded rat kidney stained for Cadherin 16 using ab270263 at 2 μg/ml in imunnohistochemical analysis.

Carrier free

Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

CDH16/1532R

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt, IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CDH16.

O75309

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified by Protein A.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cadherin 16 also known as Ksp-cadherin is a calcium-dependent cell adhesion protein. It plays a role in mediating homophilic cell-cell adhesion facilitating the binding between identical cadherins on neighboring cells. The mass of cadherin 16 is approximately 92 kDa. This protein is mainly expressed in the kidney specifically in the distal convoluted tubules and collecting ducts where it is localized to the basolateral membrane of tubular epithelial cells.

Biological function summary

Cadherin 16 contributes to cell sorting and tissue morphogenesis. It does not operate as part of a multi-protein complex but interacts with the actin cytoskeleton through catenins like β-catenin to maintain cell adhesion and tissue integrity. The particular expression pattern of cadherin 16 suggests that it has a role in maintaining the structural organization of renal tissues and possibly influences renal tubular epithelial homeostasis.

Pathways

Cadherin 16 participates in cell signaling pathways essential for cell adhesion and tissue architecture. Specifically it engages in the Wnt signaling pathway where it coordinates with proteins like β-catenin indicating its indirect role in signal transduction that may affect gene transcription regarding cell growth and differentiation. Another key interaction for cadherin 16 is with integrins which are pivotal in mediating attachments between the extracellular matrix and the cell.

Cadherin 16 is linked to renal-related conditions such as renal cell carcinoma and polycystic kidney disease. Abnormal expression or dysfunction of cadherin 16 could disrupt cell adhesion promoting renal cell carcinoma by facilitating cell detachment and metastatic progression. Additionally it connects with proteins like E-cadherin which are involved in epithelial-to-mesenchymal transition (EMT) a process often activated in cancer development and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

See full target information CDH16

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 134: PubMed39145457

2024

Posttranslationally modified self-peptides promote hypertension in mouse models.

Applications

Unspecified application

Species

Unspecified reactive species

Nathaniel Bloodworth,Wei Chen,Kuniko Hunter,David Patrick,Amy Palubinsky,Elizabeth Phillips,Daniel Roeth,Markus Kalkum,Simon Mallal,Sean Davies,Mingfang Ao,Rocco Moretti,Jens Meiler,David G Harrison

Frontiers in immunology 13:1008195 PubMed36268034

2022

MiR-585-5p impedes gastric cancer proliferation and metastasis by orchestrating the interactions among CREB1, MAPK1 and MITF.

Applications

Unspecified application

Species

Unspecified reactive species

Yunwei Wang,Ming Li,Jiaoxia Zeng,Yunshu Yang,Zengshan Li,Sijun Hu,Fangfang Yang,Na Wang,Wenlan Wang,Jun Tie

Theranostics 11:10064-10073 PubMed34815804

2021

Heterogeneity of cell composition and origin identified by single-cell transcriptomics in renal cysts of patients with autosomal dominant polycystic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Li,Yuchen Wang,Wenfeng Deng,Yanna Liu,Jian Geng,Ziyan Yan,Fei Li,Binshen Chen,Zhuolin Li,Renfei Xia,Wenli Zeng,Rumin Liu,Jian Xu,Fu Xiong,Chin-Lee Wu,Yun Miao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cadherin 16 antibody [CDH16/1532R]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] (AB270263)

Flow Cytometry - Anti-Cadherin 16 antibody [CDH16/1532R] (AB270263)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] (AB270263)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] (AB270263)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise