AB270294

Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free

Recombinant
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Rabbit Recombinant Monoclonal Cadherin 16 antibody. Carrier free. Suitable for Flow Cyt, WB, IHC-P and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CDH16.

View Alternative Names

UNQ695/PRO1340, CDH16, Cadherin-16, Kidney-specific cadherin, Ksp-cadherin

4 Images

Flow Cytometry - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (AB270294)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (AB270294)

Flow cytometric analysis of HEK-293 (Human epithelial cell line from embryonic kidney) cells labeling Cadherin 16 (Blue) using ab270263 at 2μg/10⁶ cells followed by goat anti-rabbit IgG-CF488; isotype control (Red).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab270263).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (AB270294)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (AB270294)

Formalin-fixed, paraffin-embedded human renal cell carcinoma stained for Cadherin 16 using ab270263 at 2 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab270263).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (AB270294)

Formalin-fixed, paraffin-embedded rat kidney tissue stained for Cadherin 16 using ab270263 at 2 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab270263).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (AB270294)

Formalin-fixed, paraffin-embedded mouse kidney tissue stained for Cadherin 16 using ab270263 at 2 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab270263).

Unconjugated

Anti-Cadherin 16 antibody [CDH16/1532R]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

CDH16/1532R

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, Flow Cyt

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CDH16.

O75309

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

ab270294 is a carrier free version of ab270263.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cadherin 16 also known as Ksp-cadherin is a calcium-dependent cell adhesion protein. It plays a role in mediating homophilic cell-cell adhesion facilitating the binding between identical cadherins on neighboring cells. The mass of cadherin 16 is approximately 92 kDa. This protein is mainly expressed in the kidney specifically in the distal convoluted tubules and collecting ducts where it is localized to the basolateral membrane of tubular epithelial cells.

Biological function summary

Cadherin 16 contributes to cell sorting and tissue morphogenesis. It does not operate as part of a multi-protein complex but interacts with the actin cytoskeleton through catenins like β-catenin to maintain cell adhesion and tissue integrity. The particular expression pattern of cadherin 16 suggests that it has a role in maintaining the structural organization of renal tissues and possibly influences renal tubular epithelial homeostasis.

Pathways

Cadherin 16 participates in cell signaling pathways essential for cell adhesion and tissue architecture. Specifically it engages in the Wnt signaling pathway where it coordinates with proteins like β-catenin indicating its indirect role in signal transduction that may affect gene transcription regarding cell growth and differentiation. Another key interaction for cadherin 16 is with integrins which are pivotal in mediating attachments between the extracellular matrix and the cell.

Cadherin 16 is linked to renal-related conditions such as renal cell carcinoma and polycystic kidney disease. Abnormal expression or dysfunction of cadherin 16 could disrupt cell adhesion promoting renal cell carcinoma by facilitating cell detachment and metastatic progression. Additionally it connects with proteins like E-cadherin which are involved in epithelial-to-mesenchymal transition (EMT) a process often activated in cancer development and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

See full target information CDH16

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free

Flow Cytometry - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (AB270294)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (AB270294)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (AB270294)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (AB270294)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise