AB214092

Anti-Cadherin 16 antibody [EPR13090] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal Cadherin 16 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications.

View Alternative Names

UNQ695/PRO1340, CDH16, Cadherin-16, Kidney-specific cadherin, Ksp-cadherin

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [EPR13090] - BSA and Azide free (AB214092)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [EPR13090] - BSA and Azide free (AB214092)

Immunohistochemical analysis of paraffin-embedded Human chromophobe renal cell carcinoma tissue labeling Cadherin 16 with ab183745 at 1/4000 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183745).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-Cadherin 16 antibody [EPR13090] - BSA and Azide free (AB214092)

Western blot analysis of Cadherin 16 in Human fetal kidney lysate immunoprecipitated using ab183745 at 1/50 dilution.

Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183745).

All lanes:

Immunoprecipitation - Anti-Cadherin 16 antibody [EPR13090] (<a href='/en-us/products/primary-antibodies/cadherin-16-antibody-epr13090-ab183745'>ab183745</a>)

Predicted band size: 90 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [EPR13090] - BSA and Azide free (AB214092)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Cadherin 16 with ab183745 at 1/4000 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183745).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Unconjugated

Anti-Cadherin 16 antibody [EPR13090]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13090

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab214092 is the carrier-free version of ab183745.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cadherin 16 also known as Ksp-cadherin is a calcium-dependent cell adhesion protein. It plays a role in mediating homophilic cell-cell adhesion facilitating the binding between identical cadherins on neighboring cells. The mass of cadherin 16 is approximately 92 kDa. This protein is mainly expressed in the kidney specifically in the distal convoluted tubules and collecting ducts where it is localized to the basolateral membrane of tubular epithelial cells.

Biological function summary

Cadherin 16 contributes to cell sorting and tissue morphogenesis. It does not operate as part of a multi-protein complex but interacts with the actin cytoskeleton through catenins like β-catenin to maintain cell adhesion and tissue integrity. The particular expression pattern of cadherin 16 suggests that it has a role in maintaining the structural organization of renal tissues and possibly influences renal tubular epithelial homeostasis.

Pathways

Cadherin 16 participates in cell signaling pathways essential for cell adhesion and tissue architecture. Specifically it engages in the Wnt signaling pathway where it coordinates with proteins like β-catenin indicating its indirect role in signal transduction that may affect gene transcription regarding cell growth and differentiation. Another key interaction for cadherin 16 is with integrins which are pivotal in mediating attachments between the extracellular matrix and the cell.

Cadherin 16 is linked to renal-related conditions such as renal cell carcinoma and polycystic kidney disease. Abnormal expression or dysfunction of cadherin 16 could disrupt cell adhesion promoting renal cell carcinoma by facilitating cell detachment and metastatic progression. Additionally it connects with proteins like E-cadherin which are involved in epithelial-to-mesenchymal transition (EMT) a process often activated in cancer development and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

See full target information CDH16

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 21:2083-2100 PubMed40083708

2025

Thrombospondin 1 Promotes Cytoskeleton Remodeling, Dedifferentiation, and Pulmonary Metastasis through ITGA1 and ITGA6 in Osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Enjie Xu,Zhen Huang,Kunpeng Zhu,Jianping Hu,Xiaolong Ma,Yongjie Wang,Jiazhuang Zhu,Chunlin Zhang

Scientific reports 13:12917 PubMed37558687

2023

Cadherin-16 (CDH16) immunohistochemistry: a useful diagnostic tool for renal cell carcinoma and papillary carcinomas of the thyroid.

Applications

Unspecified application

Species

Unspecified reactive species

Maximilian Lennartz,Henrietta Csomós,Viktoria Chirico,Sören Weidemann,Natalia Gorbokon,Anne Menz,Franziska Büscheck,Claudia Hube-Magg,Doris Höflmayer,Christian Bernreuther,Niclas C Blessin,Patrick Lebok,Guido Sauter,Stefan Steurer,Eike Burandt,David Dum,Till Krech,Ronald Simon,Sarah Minner,Frank Jacobsen,Till S Clauditz,Andreas M Luebke,Abdul Khalid Siraj,Fouad Al-Dayel,Khawla S Al-Kuraya,Andrea Hinsch

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cadherin 16 antibody [EPR13090] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [EPR13090] - BSA and Azide free (AB214092)

Immunoprecipitation - Anti-Cadherin 16 antibody [EPR13090] - BSA and Azide free (AB214092)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [EPR13090] - BSA and Azide free (AB214092)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise