Rabbit Polyclonal Cadherin 7 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CDH7 aa 100-300.
View Alternative Names
CDH7L1, CDH7, Cadherin-7
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Cadherin 7 antibody (AB237754)
A549 (Human lung carcinoma cell line) cells stained for Cadherin 7 (green) using ab237754 at 1/133 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 7 antibody (AB237754)
Paraffin-embedded human colon cancer tissue stained for Cadherin 7 using ab237754 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
- WB
Supplier Data
Western blot - Anti-Cadherin 7 antibody (AB237754)
All lanes:
Western blot - Anti-Cadherin 7 antibody (ab237754) at 1/500 dilution
Lane 1:
HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2:
HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3:
K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Secondary
All lanes:
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 87 kDa
false
Reactivity data
Properties and storage information
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein mediates strong stable adhesion among cells in the neural tissue. It is integral to the assembly of adherens junctions where it participates in cell signaling and structural integrity. Cadherin 7 does not function alone; it often forms complexes with cytoplasmic proteins such as catenins which link the cadherin to the actin cytoskeleton providing mechanical support and facilitating intracellular signaling.
Pathways
Cadherin 7 plays a critical role in pathways like the cadherin-catenin complex and the Wnt signaling pathway. The cadherin-catenin complex pathway modulates cell polarity and gene expression by interacting with proteins like β-catenin. In the Wnt signaling pathway Cadherin 7 helps regulate cell fate proliferation and migration which are all vital for neural development and tissue homeostasis.
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Target data
Publications (1)
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World journal of gastroenterology 29:3770-3792 PubMed37426316
2023
Applications
Unspecified application
Species
Unspecified reactive species
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