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AB323816

Anti-Caffeine antibody [EPR27992-89] - BSA and Azide free

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Rabbit Recombinant Monoclonal Caffeine antibody. Carrier free. Suitable for cELISA and reacts with Chemical samples.

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A1AR, A1R, A3AR, A3R, AA1R_HUMAN, AA3R_HUMAN, ACEE, ACES_HUMAN, ACLS, AD 026, ADORA 1, ADORA 3, AIS, ANDR_HUMAN, AR, AR8, ARA3, ARACHE, Acetylcholinesterase, Adenosine A3 receptor, Adenosine receptor A1, Adenosine receptor A3, Androgen nuclear receptor variant 2, Androgen receptor, Androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease), Apoptosis related acetylcholinesterase, Atherosclerosis, susceptibility to, included, BXR, DHTR, DKFZp686N23123, DNA binding protein, Dihydro testosterone receptor, Dihydrotestosterone receptor (DHTR), ER, ER-alpha, ER-beta, ERR a, ERR-alpha, ERR1 protein, ERR1_HUMAN, ER[a], ER[b], ESR, ESR B, ESR BETA, ESR1_HUMAN, ESRA, ESRL 1, ESRR A, ESTR B, Eag-related protein 1, Era, Erb, Erb2, Estr, Estra, Estradiol Receptor alpha, Estradiol Receptor beta, Estradiol receptor, Estrogen Receptor 1, Estrogen Receptor 2, Estrogen receptor, Estrogen receptor 1 (alpha), Estrogen receptor 2 (ER beta), Estrogen receptor 2 ER beta, Estrogen receptor alpha, Estrogen receptor beta 4, Estrogen receptor related 1, Estrogen receptor-like 1, Estrogen resistance, included, Estrogen-related receptor alpha, Estrra, Ether a go go related potassium channel protein, Ether-a-go-go-related gene potassium channel 1, Ether-a-go-go-related protein 1, GCPS, GLI Kruppel family member GLI 3, GLI Kruppel family member GLI3 (Greig cephalopolysyndactyly syndrome), GLI family zinc finger 3, GLI3 C-terminally truncated form, GLI3 form of 190 kDa, GLI3 form of 83 kDa, GLI3 full length protein, GLI3-190, GLI3-83, GLI3FL, GLI3_HUMAN, GPCR 2, Glioma associated oncogene family zinc finger 3, H-ERG, HDL cholesterol, augmented response of, to hormone replacement, included, HUMARA, HYSP1, IMD42, KCNH2_HUMAN, KD, Kennedy disease (KD), Kv11.1, LQT 2, MGC129539, Myocardial infarction, susceptibility to, included, N-ACHE, NR1F3, NR1I2_HUMAN, NR3A1, NR3A2, NR3B1, NR3C4, Nuclear receptor ROR-gamma, Nuclear receptor RZR-gamma, Nuclear receptor subfamily 1 group F member 3, Nuclear receptor subfamily 1 group I member 2, Nuclear receptor subfamily 3 group A member 1, Nuclear receptor subfamily 3 group A member 2, Nuclear receptor subfamily 3 group B member 1, Nuclear receptor subfamily 3 group C member 4, Nuclear receptor subfamily 3 group C member 4 (NR3C4), ONR 1, OTTHUMP00000017718, OTTHUMP00000017719, OTTHUMP00000215173, OTTHUMP00000215174, OTTHUMP00000215175, Oncogene GLI3, Orphan nuclear receptor PAR 1, Orphan nuclear receptor PXR, PAP A, PAPB, PAR, PAR q, PPD IV, PRR, Potassium channel HERG, Potassium voltage gated channel subfamily H (eag related) member 2, Potassium voltage-gated channel subfamily H member 2, Pregnane X receptor, RAR related orphan nuclear receptor variant 2, RAR related orphan receptor C, isoform a, RAR related orphan receptor gamma, RAR-related orphan receptor C, RDC 7, RNESTROR, RORG_HUMAN, RP11 552M11.7, RZR GAMMA, RZRG, Retinoic acid binding receptor gamma, Retinoid-related orphan receptor-gamma, Ri, Rorc, SBMA, SMAX1, SQT1, SXR, Spinal and bulbar muscular atrophy, Spinal and bulbar muscular atrophy (SBMA), Steroid and xenobiotic receptor, Steroid hormone receptor ERR1, TFM, TGPCR1, TOR, Testicular Feminization (TFM), Transcriptional activator GLI3, Transcriptional repressor GLI3R, Voltage gated potassium channel, subfamily H, member 2, Voltage-gated potassium channel subunit Kv11.1, YT, YT blood group, Zinc finger protein GLI 3, androgen receptor splice variant 4b, bA552M11.5, eag homolog, estrogen receptor related receptor alpha, hERG-1, hERR1, pregnane X nuclear receptor variant 2

2 Images
Competitive ELISA - Anti-Caffeine antibody [EPR27992-89] - BSA and Azide free (AB323816)
  • cELISA

Supplier Data

Competitive ELISA - Anti-Caffeine antibody [EPR27992-89] - BSA and Azide free (AB323816)

ab323816 (0.25 µg/mL) is specific to Caffeine and the percent cross reactivity with other compounds is demonstrated in the table.

Competitive ELISA - Anti-Caffeine antibody [EPR27992-89] - BSA and Azide free (AB323816)
  • cELISA

Supplier Data

Competitive ELISA - Anti-Caffeine antibody [EPR27992-89] - BSA and Azide free (AB323816)

1 µg/mL Caffeine-crosslink-BSA was coated onto 96-wells. Serial dilution of free Caffeine (0, 4, 8, 16, 32, 64, 128, 256, 512 ng/mL) was added (50 µL). This was followed by adding 0.25 µg/mL ab323816 (50 µL) into each well. The plate was incubated for 30 mins. After washing, 1 : 20000 diluted GAR Fc-HRP was added and incubated for 30 mins. After washing, 100 µL of TMB was added and incubated for 5-10 mins without shaking for color development. Then 100 µL of stop solution (4.9% Orthophosphoric acid) was dispensed and OD was read at 450nm on a microplate reader within 5-10 mins.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27992-89

Isotype

IgG

Carrier free

Yes

Applications

cELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody has 0.11% cross reaction with 1, 3, 7-Trimethyluric acid, 52.88% cross reaction with theophylline, 0.44% cross reaction with pentoxifylline, 3.23% cross reaction with theobromine and 0.06% cross reaction with acefylline.

Working range: 4-512 ng/mL.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "cELISA" : {"fullname" : "Competitive ELISA", "shortname":"cELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "cELISA-species-checked": "testedAndGuaranteed", "cELISA-species-dilution-info": "", "cELISA-species-notes": "<p></p>" } } }

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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