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AB325663

Anti-CALB1 antibody [L109/57] - Chicken IgY (Chimeric)

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Chicken Monoclonal Calbindin antibody. Suitable for IHC-P, WB and reacts with Rat samples.

View Alternative Names

Calbindin, Calbindin D28, D-28K, Spot 35 protein

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CALB1 antibody [L109/57] - Chicken IgY (Chimeric) (AB325663)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CALB1 antibody [L109/57] - Chicken IgY (Chimeric) (AB325663)

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of Purkinje cells with ab325663 at 1 : 100 dilution (green). The stained sections were mounted with Fluoroshield with DAPI and DABCO mounting medium. DAPI nuclear stain (blue) shows cell nuclei. The antibody specifically stains the Purkinje cells in the cerebellum. Magnification 100X.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CALB1 antibody [L109/57] - Chicken IgY (Chimeric) (AB325663)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CALB1 antibody [L109/57] - Chicken IgY (Chimeric) (AB325663)

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of Purkinje cells by ab325663 at 1 : 100 dilution (green). The stained sections were mounted with Fluoroshield with DAPI and DABCO mounting medium. DAPI nuclear stain (blue) shows cell nuclei. The antibody specifically stains the Purkinje cells in the cerebellum. Magnification 400X.

Western blot - Anti-CALB1 antibody [L109/57] - Chicken IgY (Chimeric) (AB325663)
  • WB

Supplier Data

Western blot - Anti-CALB1 antibody [L109/57] - Chicken IgY (Chimeric) (AB325663)

Western blotting of rat brain homogenate with an anti-CALB1 (calbindin) mouse mAb and ab325663 antibodies (10 µg/ml). The mouse Mab and ab325663 were detected with anti-mouse-HRP and anti-chicken-HRP, respectively. The antibody recognizes endogenous CALB1 with similar sensitivity as the anti-CALB1 mouse Mab in the rat brain homogenate at 30 kDa.

All lanes:

Western blot - Anti-CALB1 antibody [L109/57] - Chicken IgY (Chimeric) (ab325663) at 10 µg/mL

All lanes:

rat brain homogenate

Secondary

All lanes:

anti-chicken-HRP

Observed band size: 30 kDa

false

Key facts

Host species

Chicken

Clonality

Monoclonal

Clone number

L109/57

Isotype

IgY

Carrier free

No

Reacts with

Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

No cross-reactivity reported.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "10 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L109/57 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L109/57 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
See full target information Calb1
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Product promise

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