Anti-Calbindin antibody [5A9]

• IHC-FoFr

Supplier Data

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Calbindin antibody [5A9] (AB315202)

Immunofluorescent analysis of rat brain cerebellum section stained with ab315202, dilution 1 : 2,000, in red, and costained with rabbit pAb to GFAP, RPCA-GFAP, dilution 1 : 5,000 in green. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with the above antibodies. The ab315202 calbindin antibody prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum. The GFAP antibody stains the processes of Bergmann glia in the molecular layer and astroglia in the granular and white matter layers of cerebellum.

• WB

Supplier Data

Western blot - Anti-Calbindin antibody [5A9] (AB315202)

Western blot analysis of different neuronal tissue lysates using ab315202, dilution 1 : 5,000 : [1] protein standard, [2] rat cerebellum, [3] pig hippocampus, and [4] cow cerebellum. Bands at about 26kDa correspond to calbindin protein, heavily expressed in the cerebellum.

All lanes:

Western blot - Anti-Calbindin antibody [5A9] (ab315202) at 1/5000 dilution

Lane 2:

Rat cerebellum tissue lysate

Lane 3:

Pig hippocampus tissue lysate

Lane 4:

Cow cerebellum tissue lysate

Observed band size: 26 kDa

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