JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB156812

Anti-Calbindin antibody

5

(2 Reviews)

|

(3 Publications)

Goat Polyclonal Calbindin antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CALB1 aa 50-100.

View Alternative Names

CAB27, CALB1, Calbindin, Calbindin D28, D-28K

2 Images
Western blot - Anti-Calbindin antibody (AB156812)
  • WB

Unknown

Western blot - Anti-Calbindin antibody (AB156812)

All lanes:

Western blot - Anti-Calbindin antibody (ab156812) at 0.03 µg/mL

All lanes:

Human Brain Cerebellum lysate (in RIPA buffer). at 35 µg

Predicted band size: 30 kDa

Observed band size: 26 kDa

true

Western blot - Anti-Calbindin antibody (AB156812)
  • WB

Supplier Data

Western blot - Anti-Calbindin antibody (AB156812)

Primary incubation was 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-Calbindin antibody (ab156812) at 0.1 µg/mL

Lane 1:

Mouse brain tissue lysate at 35 µg

Lane 2:

rat brain tissue lysate at 35 µg

Predicted band size: 30 kDa

Observed band size: 30 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human CALB1 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

P05937

style
left-column

Alternative Products

Primary Antibodies

AB108404

Anti-Calbindin antibody [EP3478]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-Calbindin antibody [EP3478] (AB108404)

  • 4
  • 3
Primary Antibodies

AB82812

Anti-Calbindin antibody [CB-955]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [CB-955] (AB82812)

  • 4
  • 8
Primary Antibodies

AB233018

Anti-Calbindin antibody [EP3478] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-Calbindin antibody [EP3478] - BSA and Azide free (AB233018)

  • 5
  • 1
Primary Antibodies

AB208377

Alexa Fluor® 488 Anti-Calbindin antibody [EP3478]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Calbindin antibody [EP3478] (AB208377)

  • 0
  • 0
Primary Antibodies

AB207528

Alexa Fluor® 647 Anti-Calbindin antibody [EP3478]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Calbindin antibody [EP3478] (AB207528)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB92341

Anti-Calretinin antibody [EP1798]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [EP1798] (AB92341)

  • 4
  • 5
Primary Antibodies

AB177487

Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

  • 5
  • 74
Primary Antibodies

AB137869

Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (AB137869)

  • 5
  • 9
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03-0.1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03-0.1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03-0.1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column

Product details

ab156812 overlaps EF hand 2, but not any calcium binding site.
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab156812 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calbindin also known as calbindin-D28k is a calcium-binding protein with a molecular mass of approximately 28 kDa. This target is mainly expressed in the neurons of the brain such as in the cerebellum and hippocampus as well as in the kidneys and intestine. Calbindin plays a mechanical role in binding and buffering intracellular calcium ions which is important for the regulation of calcium-dependent signaling pathways. Researchers often use techniques like Calbindin IHC (immunohistochemistry) and ELISA to study its distribution and presence within tissues.
Biological function summary

Calbindin contributes to cellular homeostasis by managing intracellular calcium levels. It does not appear to be part of a larger protein complex but operates independently. This protein helps to protect neurons from calcium overload potentially safeguarding against excitotoxicity which occurs when neurons die from excessive stimulation. Calbindin's function as a calcium buffer is of particular importance in maintaining neuron function and survival in areas with high neuronal activity.

Pathways

Calbindin has a significant role in the calcium signaling pathway and is also involved in the vitamin D pathway. The calcium signaling pathway is vital for many cellular processes like muscle contraction neurotransmitter release and gene expression. The vitamin D pathway regulates calcium and phosphate homeostasis in the body. Calbindin's relationship with proteins such as calmodulin and parvalbumin both involved in calcium regulation highlights its importance in maintaining cellular functions.

Abnormal Calbindin expression has been observed in conditions like epilepsy and Parkinson's disease. In epilepsy its decreased expression can impact the ability of neurons to regulate calcium properly leading to increased vulnerability to seizures. In Parkinson's disease altered Calbindin levels have been associated with the selective vulnerability of dopamine neurons. These diseases highlight the connection between Calbindin and proteins like alpha-synuclein known for its role in Parkinson's pathology emphasizing the need to understand its contribution to disease mechanisms.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
See full target information CALB1
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:19831 PubMed37963945

2023

Developmental and foliation changes due to dysregulation of adenosine kinase in the cerebellum.

Applications

Unspecified application

Species

Unspecified reactive species

Hoda M Gebril,Tho Lai,Denise E Fedele,Amir Wahba

eNeuro 8: PubMed33863781

2021

Developmental Role of Adenosine Kinase in the Cerebellum.

Applications

Unspecified application

Species

Unspecified reactive species

Hoda Gebril,Amir Wahba,Xiaofeng Zhou,Tho Lai,Enmar Alharfoush,Emanuel DiCicco-Bloom,Detlev Boison

Cell death discovery 7:57 PubMed33753742

2021

The oncogene Mct-1 promotes progression of hepatocellular carcinoma via enhancement of Yap-mediated cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjie Yang,Yong Ni,Shikun Yang,Yang Ji,Xinchen Yang,Feng Cheng,Xuehao Wang,Feng Zhang,Jianhua Rao
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com