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AB82812

Anti-Calbindin antibody [CB-955]

4

(8 Reviews)

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(69 Publications)

Anti-Calbindin antibody [CB-955] (ab82812) is a mouse monoclonal antibody detecting Calbindin in Western Blot, IHC-P. Suitable for Cow, Human, Mouse, Rat.

- Over 50 publications
- Trusted since 2009

View Alternative Names

Calbindin, Calbindin D28, D-28K, CALB1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [CB-955] (AB82812)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [CB-955] (AB82812)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebellum tissue labelling Calbindin with ab82812 at 10 μg/mL.

Western blot - Anti-Calbindin antibody [CB-955] (AB82812)
  • WB

Supplier Data

Western blot - Anti-Calbindin antibody [CB-955] (AB82812)

Lane 1:

Western blot - Anti-Calbindin antibody [CB-955] (ab82812) at 4 µg/mL

Lane 2:

No primary control

All lanes:

MDBK cell extract

Secondary

All lanes:

Goat Anti-Mouse IgG (HRP)

Predicted band size: 30 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CB-955

Isotype

IgG1

Carrier free

No

Reacts with

Rat, Cow, Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Cow CALB1. The exact immunogen used to generate this antibody is proprietary information.

P04467

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "2-4 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "10-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "2-4 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "10-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2-4 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-Calbindin antibody [CB-955] (ab82812) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Cow, Human, Mouse, Rat samples.

Trusted by the scientific community
Anti-Calbindin [CB-955] (ab82812) was first used in a scientific publication in 2009 and has been cited over 50 times in peer-reviewed journals.

Reviewed by scientists
Anti-Calbindin [CB-955] (ab82812) has over 5 independent reviews from customers.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified from hybridoma cell culture is Protein A column.
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calbindin also known as calbindin-D28k is a calcium-binding protein with a molecular mass of approximately 28 kDa. This target is mainly expressed in the neurons of the brain such as in the cerebellum and hippocampus as well as in the kidneys and intestine. Calbindin plays a mechanical role in binding and buffering intracellular calcium ions which is important for the regulation of calcium-dependent signaling pathways. Researchers often use techniques like Calbindin IHC (immunohistochemistry) and ELISA to study its distribution and presence within tissues.
Biological function summary

Calbindin contributes to cellular homeostasis by managing intracellular calcium levels. It does not appear to be part of a larger protein complex but operates independently. This protein helps to protect neurons from calcium overload potentially safeguarding against excitotoxicity which occurs when neurons die from excessive stimulation. Calbindin's function as a calcium buffer is of particular importance in maintaining neuron function and survival in areas with high neuronal activity.

Pathways

Calbindin has a significant role in the calcium signaling pathway and is also involved in the vitamin D pathway. The calcium signaling pathway is vital for many cellular processes like muscle contraction neurotransmitter release and gene expression. The vitamin D pathway regulates calcium and phosphate homeostasis in the body. Calbindin's relationship with proteins such as calmodulin and parvalbumin both involved in calcium regulation highlights its importance in maintaining cellular functions.

Abnormal Calbindin expression has been observed in conditions like epilepsy and Parkinson's disease. In epilepsy its decreased expression can impact the ability of neurons to regulate calcium properly leading to increased vulnerability to seizures. In Parkinson's disease altered Calbindin levels have been associated with the selective vulnerability of dopamine neurons. These diseases highlight the connection between Calbindin and proteins like alpha-synuclein known for its role in Parkinson's pathology emphasizing the need to understand its contribution to disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
See full target information CALB1
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Publications (69)

Recent publications for all applications. Explore the full list and refine your search

The Journal of neuroscience : the official journal of the Society for Neuroscience 45: PubMed40015986

2025

Molecular and Anatomical Strengthening of "Winner" Climbing Fiber Synapses in Developing Mouse Purkinje Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Asako Nitta,Miwako Yamasaki,Taisuke Miyazaki,Kohtarou Konno,Haruto Yoshimura,Masahiko Watanabe

Angiogenesis 28:11 PubMed39756006

2025

Ref-1 is overexpressed in neovascular eye disease and targetable with a novel inhibitor.

Applications

Unspecified application

Species

Unspecified reactive species

Anbukkarasi Muniyandi,Gabriella D Hartman,Kamakshi Sishtla,Ratan Rai,Cátia Gomes,Kristina Day,Yang Song,Andi R Masters,Sara K Quinney,Xiaoping Qi,Hailey Woods,Michael E Boulton,Jason S Meyer,Jonah Z Vilseck,Millie M Georgiadis,Mark R Kelley,Timothy W Corson

EMBO molecular medicine 16:2882-2917 PubMed39358605

2024

Pre-clinical development of AP4B1 gene replacement therapy for hereditary spastic paraplegia type 47.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica P Wiseman,Joseph M Scarrott,João Alves-Cruzeiro,Afshin Saffari,Cedric Böger,Evangelia Karyka,Emily Dawes,Alexandra K Davies,Paolo M Marchi,Emily Graves,Fiona Fernandes,Zih-Liang Yang,Ian Coldicott,Jennifer Hirst,Christopher P Webster,J Robin Highley,Neil Hackett,Adrienn Angyal,Thushan de Silva,Adrian Higginbottom,Pamela J Shaw,Laura Ferraiuolo,Darius Ebrahimi-Fakhari,Mimoun Azzouz

STAR protocols 5:102936 PubMed38735042

2024

Generation and enrichment of cerebellar GABAergic interneurons from human induced pluripotent stem cells and intracellular calcium measurements.

Applications

Unspecified application

Species

Unspecified reactive species

Federica Pilotto,Rim Diab,Zahraa Al Qassab,Smita Saxena

Cancer cell 41:1989-2005.e9 PubMed37802055

2023

Single-cell mapping identifies MSI cells as a common origin for diverse subtypes of pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Nirakar Rajbhandari,Michael Hamilton,Cynthia M Quintero,L Paige Ferguson,Raymond Fox,Christian M Schürch,Jun Wang,Mari Nakamura,Nikki K Lytle,Matthew McDermott,Emily Diaz,Hannah Pettit,Marcie Kritzik,Haiyong Han,Derek Cridebring,Kwun Wah Wen,Susan Tsai,Michael G Goggins,Andrew M Lowy,Robert J Wechsler-Reya,Daniel D Von Hoff,Aaron M Newman,Tannishtha Reya

Neuron 111:2523-2543.e10 PubMed37321222

2023

Early molecular layer interneuron hyperactivity triggers Purkinje neuron degeneration in SCA1.

Applications

Unspecified application

Species

Unspecified reactive species

Federica Pilotto,Christopher Douthwaite,Rim Diab,XiaoQian Ye,Zahraa Al Qassab,Christoph Tietje,Meriem Mounassir,Adolfo Odriozola,Aishwarya Thapa,Ronald A M Buijsen,Sophie Lagache,Anne-Christine Uldry,Manfred Heller,Stefan Müller,Willeke M C van Roon-Mom,Benoît Zuber,Sabine Liebscher,Smita Saxena

Molecular vision 29:39-57 PubMed37287644

2023

Encephalopsin (OPN3) is required for normal refractive development and the GO/GROW response to induced myopia.

Applications

Unspecified application

Species

Unspecified reactive species

Courtney Linne,Khine Yin Mon,Shane D'Souza,Heonuk Jeong,Xiaoyan Jiang,Dillon M Brown,Kevin Zhang,Shruti Vemaraju,Kazuo Tsubota,Toshihide Kurihara,Machelle T Pardue,Richard A Lang

American journal of physiology. Cell physiology 324:C1171-C1178 PubMed37036298

2023

Bicarbonate is the primary inducer of KCC3a expression in renal cortical B-type intercalated cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammed Z Ferdaus,Andrew S Terker,Rainelli Koumangoye,Susan M Wall,Eric Delpire

Frontiers in physiology 14:1154694 PubMed37082243

2023

Dietary sodium enhances the expression of SLC4 family transporters, IRBIT, L-IRBIT, and PP1 in rat kidney: Insights into the molecular mechanism for renal sodium handling.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Cai,Dengke Wang,Tianxiang Gui,Xiaoyu Wang,Lingyu Zhao,Walter F Boron,Li-Ming Chen,Ying Liu

Brain communications 5:fcac335 PubMed36632189

2023

Ap4b1-knockout mouse model of hereditary spastic paraplegia type 47 displays motor dysfunction, aberrant brain morphology and ATG9A mislocalization.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph M Scarrott,João Alves-Cruzeiro,Paolo M Marchi,Christopher P Webster,Zih-Liang Yang,Evangelia Karyka,Raffaele Marroccella,Ian Coldicott,Hannah Thomas,Mimoun Azzouz
View all publications
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