AB317108

Anti-Calbindin + Calretinin antibody [HL1787]

Recombinant
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Rabbit Recombinant Monoclonal Calbindin antibody. Suitable for WB, IHC-P, IHC-Fr, ICC/IF and reacts with Transfected cell lysate, Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human CALB1.

View Alternative Names

CAB27, CALB1, Calbindin, Calbindin D28, D-28K

6 Images

Immunohistochemistry (Frozen sections) - Anti-Calbindin + Calretinin antibody [HL1787] (AB317108)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Calbindin + Calretinin antibody [HL1787] (AB317108)

ab317108 detects Calbindin + Calretinin protein at cytoplasm by immunohistochemical analysis.
Sample : Frozen-sectioned mouse cerebellum.
Green : Calbindin + Calretinin stained by ab317108 diluted at 1 : 100.
Blue : Fluoroshield with DAPI.

Immunocytochemistry/ Immunofluorescence - Anti-Calbindin + Calretinin antibody [HL1787] (AB317108)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Calbindin + Calretinin antibody [HL1787] (AB317108)

ab317108 detects Calbindin protein at cytoskeleton by immunofluorescent analysis.
Sample : DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green : Calbindin stained by ab317108 diluted at 1 : 250.
Red : Tau an axon marker stained by Tau antibody diluted at 1 : 500.
Blue : Fluoroshield with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin + Calretinin antibody [HL1787] (AB317108)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin + Calretinin antibody [HL1787] (AB317108)

ab317108 detects Calbindin protein at cytoplasm by immunohistochemical analysis.
Sample : Paraffin-embedded rat cerebellum.
Green : Calbindin stained by ab317108 diluted at 1 : 250.
Red : NF-H a neural marker stained by NF-H antibody diluted at 1 : 500.
Blue : Fluoroshield with DAPI.
Antigen Retrieval : Citrate buffer pH 6.0 15 min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin + Calretinin antibody [HL1787] (AB317108)

ab317108 detects Calbindin protein at cytoplasm by immunohistochemical analysis.
Sample : Paraffin-embedded mouse cerebellum.
Green : Calbindin stained by ab317108 diluted at 1 : 250.
Red : NF-H a neural marker stained by NF-H antibody diluted at 1 : 500.
Blue : Fluoroshield with DAPI.
Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Supplier Data

Western blot - Anti-Calbindin + Calretinin antibody [HL1787] (AB317108)

Human tissue extract (30 ug) was separated by 12% SDS-PAGE and the membrane was blotted with ab317108 diluted at 1 : 500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-Calbindin + Calretinin antibody [HL1787] (ab317108) at 1/500 dilution

All lanes:

Human brain tissue extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

Supplier Data

Western blot - Anti-Calbindin + Calretinin antibody [HL1787] (AB317108)

Whole cell extract (30 ug) was separated by 12% SDS-PAGE and the membrane was blotted with ab317108 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody and the signal was developed with Trident femto Western HRP Substrate.

All lanes:

Western blot - Anti-Calbindin + Calretinin antibody [HL1787] (ab317108) at 1/1000 dilution

All lanes:

IMR32 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1787

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

IHC-P, ICC/IF, WB, IHC-Fr

applications

Immunogen

Recombinant Fragment Protein within Human CALB1.

P05937

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calbindin also known as calbindin-D28k is a calcium-binding protein with a molecular mass of approximately 28 kDa. This target is mainly expressed in the neurons of the brain such as in the cerebellum and hippocampus as well as in the kidneys and intestine. Calbindin plays a mechanical role in binding and buffering intracellular calcium ions which is important for the regulation of calcium-dependent signaling pathways. Researchers often use techniques like Calbindin IHC (immunohistochemistry) and ELISA to study its distribution and presence within tissues.

Biological function summary

Calbindin contributes to cellular homeostasis by managing intracellular calcium levels. It does not appear to be part of a larger protein complex but operates independently. This protein helps to protect neurons from calcium overload potentially safeguarding against excitotoxicity which occurs when neurons die from excessive stimulation. Calbindin's function as a calcium buffer is of particular importance in maintaining neuron function and survival in areas with high neuronal activity.

Pathways

Calbindin has a significant role in the calcium signaling pathway and is also involved in the vitamin D pathway. The calcium signaling pathway is vital for many cellular processes like muscle contraction neurotransmitter release and gene expression. The vitamin D pathway regulates calcium and phosphate homeostasis in the body. Calbindin's relationship with proteins such as calmodulin and parvalbumin both involved in calcium regulation highlights its importance in maintaining cellular functions.

Abnormal Calbindin expression has been observed in conditions like epilepsy and Parkinson's disease. In epilepsy its decreased expression can impact the ability of neurons to regulate calcium properly leading to increased vulnerability to seizures. In Parkinson's disease altered Calbindin levels have been associated with the selective vulnerability of dopamine neurons. These diseases highlight the connection between Calbindin and proteins like alpha-synuclein known for its role in Parkinson's pathology emphasizing the need to understand its contribution to disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.

See full target information CALB1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Calbindin + Calretinin antibody [HL1787]

Immunohistochemistry (Frozen sections) - Anti-Calbindin + Calretinin antibody [HL1787] (AB317108)

Immunocytochemistry/ Immunofluorescence - Anti-Calbindin + Calretinin antibody [HL1787] (AB317108)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin + Calretinin antibody [HL1787] (AB317108)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin + Calretinin antibody [HL1787] (AB317108)

Western blot - Anti-Calbindin + Calretinin antibody [HL1787] (AB317108)

Western blot - Anti-Calbindin + Calretinin antibody [HL1787] (AB317108)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise