AB282104

Anti-Calcineurin A antibody [EPR24997-22]

BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
KO Validated
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(8 Publications)

Rabbit Recombinant Monoclonal Calcineurin A antibody. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat, Recombinant full length protein - Human samples. Cited in 8 publications.

View Alternative Names

CALNA, CNA, PPP3CA, Protein phosphatase 3 catalytic subunit alpha, CAM-PRP catalytic subunit, Calcineurin A alpha, Calmodulin-dependent calcineurin A subunit alpha isoform, Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform, CNA alpha

15 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling Calcineurin A with ab282104 at 1/2000 dilution (0.267 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on human cerebrum (PMID : 28524178). The section was incubated with ab282104 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Immunohistochemical analysis of paraffin-embedded Parental HAP1 cell pellet (A) and PPP3CA KO HAP1 cell pellet (B) labelling Calcineurin A with ab282104 at 1/100 dilution (5.33 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on parental HAP1 cell pellet (A) and no staining on PPP3CA KO HAP1 cell pellet (B). The section was incubated with ab282104 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labelling Calcineurin A with ab282104 at 1/2000 dilution (0.267 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Cytoplamic staining on human colon carcinoma. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HeLa (Human cervix adenocarcinoma) cells labelling Calcineurin A with ab282104 at 1/50 dilution (10.66 ug/ml), followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HeLa cell line is observed.

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma) cells labelling Calcineurin A with ab282104 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labelling Calcineurin A with ab282104 at 1/2000 dilution (0.267 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Cytoplamic staining on human astrocytoma. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

Supplier Data

Immunoprecipitation - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Calcineurin A was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma) whole cell lysate with ab282104 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab282104 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (Human cervix adenocarcinoma) whole cell lysate 10 ug

Lane 2 : ab282104 IP in HeLa whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab282104 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes

All lanes:

Immunoprecipitation - Anti-Calcineurin A antibody [EPR24997-22] (ab282104)

Predicted band size: 59 kDa

Observed band size: 32 kDa,59 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling Calcineurin A with ab282104 at 1/2000 dilution (0.267 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on rat cerebrum. The section was incubated with ab282104 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling Calcineurin A with ab282104 at 1/2000 dilution (0.267 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on mouse cerebrum. The section was incubated with ab282104 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling Calcineurin A with ab282104 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling Calcineurin A with ab282104 at 1/50 dilution (10.66 ug/ml), followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in NIH/3T3 cell line is observed.

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

Supplier Data

Immunoprecipitation - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Calcineurin A was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab282104 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab282104 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate10 ug

Lane 2 : ab282104 IP in NIH/3T3 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab282104 in NIH/3T3 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 59 seconds

All lanes:

Immunoprecipitation - Anti-Calcineurin A antibody [EPR24997-22] (ab282104)

Predicted band size: 59 kDa

Observed band size: 32 kDa,59 kDa

false

Lab

Western blot - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Blocking and diluting buffer and concentration : Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS

Lanes 1-4 : Merged signal (red and green). Green - ab282104 observed at 59/32 kDa. Red - loading control ab8245 observed at 36 kDa.

ab282104 Anti-PPP3CA antibody [EPR24997-22 HT] was shown to specifically react with PPP3CA in wild-type HAP1 cells. Loss of signal was observed when the knockout cell line was used. Wild-type and PPP3CA knockout samples were subjected to SDS-PAGE. ab282104 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4℃ overnight at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

The expression pattern & observed MW are consistent with what has been described in the literature (PMID : 17785681).

All lanes:

Western blot - Anti-Calcineurin A antibody [EPR24997-22] (ab282104) at 1/1000 dilution

Lane 1:

Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate at 20 µg

Lane 2:

PPP3CA knockout HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate at 20 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/10000 dilution

Predicted band size: 59 kDa

Observed band size: 32 kDa,59 kDa

false

Lab

Western blot - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The expression pattern & observed MW are consistent with what has been described in the literature (PMID : 17785681).

Exposure time : Lane 1-2 : 3.25 seconds Lane 3 : 26 seconds Lane 4 : 59 seconds

All lanes:

Western blot - Anti-Calcineurin A antibody [EPR24997-22] (ab282104) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Lane 3:

Mouse lung tissue lysate at 20 µg

Lane 4:

Rat lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 59 kDa

Observed band size: 32 kDa,59 kDa

false

Lab

Western blot - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This antibody does not recognize human PPP3CB and PPP3CC. These rec proteins are made in-house and expressed from E.coli expression systems.

Exposure time : 15 seconds

All lanes:

Western blot - Anti-Calcineurin A antibody [EPR24997-22] (ab282104) at 1/1000 dilution

Lane 1:

Human PPP3CA recombinant protein at 0.01 µg

Lane 2:

Human PPP3CB recombinant protein at 0.01 µg

Lane 3:

Human PPP3CC recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 59 kDa

Observed band size: 32 kDa,59 kDa

false

Carrier free

Anti-Calcineurin A antibody [EPR24997-22] - BSA and Azide free
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Calcineurin A antibody [EPR24997-22]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24997-22

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, IHC-P, ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calcineurin A also known as PPP3CA is a highly specific protein phosphatase with a pivotal role in cellular signaling. It has a molecular mass of approximately 59 to 61 kDa depending on the isoform. Calcineurin A expression occurs in various tissues including the brain immune cells and cardiac tissues. It acts as the catalytic subunit of the enzyme calcineurin and is important for its function.

Biological function summary

Calcineurin A participates in numerous cellular processes through its phosphatase activity. As part of the calcineurin complex which includes a regulatory subunit it modulates the dephosphorylation of target proteins. This activity enables Calcineurin A to influence activation of T-lymphocytes by dephosphorylating the nuclear factor of activated T-cells (NFAT) a transcription factor responsible for immune response. Calcineurin A also plays a role in neuronal signaling and muscle function.

Pathways

Calcineurin A engages in the calcium signaling pathway which is vital for diverse cellular functions. It is related to proteins like calmodulin which binds calcium ions to activate Calcineurin A. Another important pathway includes the MAPK signaling pathway where Calcineurin A influences various cellular outcomes by regulating different transcription factors. Through these pathways Calcineurin A integrates signals that affect cellular growth survival and differentiation.

Calcineurin A is associated with cardiac hypertrophy and immunosuppressive conditions. In cardiac hypertrophy aberrant Calcineurin A activity leads to changes in heart muscle size and function. The protein also connects to the disorder of immunosuppression through its inhibition by drugs like cyclosporine which are used to prevent transplant rejection by targeting Calcineurin A. This inhibition blocks T-cell activation and provides therapeutic benefits in managing transplant patients.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals (PubMed : 15671020, PubMed : 18838687, PubMed : 19154138, PubMed : 23468591, PubMed : 30254215). Many of the substrates contain a PxIxIT motif and/or a LxVP motif (PubMed : 17498738, PubMed : 17502104, PubMed : 22343722, PubMed : 23468591, PubMed : 27974827). In response to increased Ca(2+) levels, dephosphorylates and activates phosphatase SSH1 which results in cofilin dephosphorylation (PubMed : 15671020). In response to increased Ca(2+) levels following mitochondrial depolarization, dephosphorylates DNM1L inducing DNM1L translocation to the mitochondrion (PubMed : 18838687). Positively regulates the CACNA1B/CAV2.2-mediated Ca(2+) release probability at hippocampal neuronal soma and synaptic terminals (By similarity). Dephosphorylates heat shock protein HSPB1 (By similarity). Dephosphorylates and activates transcription factor NFATC1 (PubMed : 19154138). In response to increased Ca(2+) levels, regulates NFAT-mediated transcription probably by dephosphorylating NFAT and promoting its nuclear translocation (PubMed : 26248042). Dephosphorylates and inactivates transcription factor ELK1 (PubMed : 19154138). Dephosphorylates DARPP32 (PubMed : 19154138). May dephosphorylate CRTC2 at 'Ser-171' resulting in CRTC2 dissociation from 14-3-3 proteins (PubMed : 30611118). Dephosphorylates transcription factor TFEB at 'Ser-211' following Coxsackievirus B3 infection, promoting nuclear translocation (PubMed : 33691586). Required for postnatal development of the nephrogenic zone and superficial glomeruli in the kidneys, cell cycle homeostasis in the nephrogenic zone, and ultimately normal kidney function (By similarity). Plays a role in intracellular AQP2 processing and localization to the apical membrane in the kidney, may thereby be required for efficient kidney filtration (By similarity). Required for secretion of salivary enzymes amylase, peroxidase, lysozyme and sialic acid via formation of secretory vesicles in the submandibular glands (By similarity). Required for calcineurin activity and homosynaptic depotentiation in the hippocampus (By similarity). Required for normal differentiation and survival of keratinocytes and therefore required for epidermis superstructure formation (By similarity). Positively regulates osteoblastic bone formation, via promotion of osteoblast differentiation (By similarity). Positively regulates osteoclast differentiation, potentially via NFATC1 signaling (By similarity). May play a role in skeletal muscle fiber type specification, potentially via NFATC1 signaling (By similarity). Negatively regulates MAP3K14/NIK signaling via inhibition of nuclear translocation of the transcription factors RELA and RELB (By similarity). Required for antigen-specific T-cell proliferation response (By similarity). Dephosphorylates KLHL3, promoting the interaction between KLHL3 and WNK4 and subsequent degradation of WNK4 (PubMed : 30718414). Negatively regulates SLC9A1 activity (PubMed : 31375679).

See full target information PPP3CA

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Chinese medicine 19:137 PubMed39369234

2024

Proteomic analysis and experimental validation reveal the blood-brain barrier protective of Huanshaodan in the treatment of SAMP8 mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yunfang Su,Ningning Liu,Pan Wang,Congcong Shang,Ruiqin Sun,Jinlian Ma,Zhonghua Li,Huifen Ma,Yiran Sun,Zijuan Zhang,Junying Song,Zhishen Xie,Jiangyan Xu,Zhenqiang Zhang

Clinics (Sao Paulo, Brazil) 79:100368 PubMed38703717

2024

TRPC5 channel participates in myocardial injury in chronic intermittent hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Mengmeng Wang,Wen Wen,Yulan Chen,Sharezati Yishajiang,Yu Li,Zhiqiang Li,Xiangyang Zhang

Journal of virology 98:e0001624 PubMed38563732

2024

The Ca-dependent phosphatase calcineurin dephosphorylates TBK1 to suppress antiviral innate immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Qu,Siyuan Wang,Hui Jiang,Qingyi Wang,Ying Liao,Xusheng Qiu,Lei Tan,Cuiping Song,Chan Ding,Yingjie Sun,Zengqi Yang

Neural regeneration research 19:2480-2487 PubMed38526284

2024

Cav3.2 channel regulates cerebral ischemia/reperfusion injury: a promising target for intervention.

Applications

Unspecified application

Species

Unspecified reactive species

Feibiao Dai,Chengyun Hu,Xue Li,Zhetao Zhang,Hongtao Wang,Wanjun Zhou,Jiawu Wang,Qingtian Geng,Yongfei Dong,Chaoliang Tang

Acta biochimica et biophysica Sinica 56:474-481 PubMed38247327

2024

Arginine vasopressin induces ferroptosis to promote heart failure via activation of the V1aR/CaN/NFATC3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyong Wu,Hua Jiang,Qiulin Yin,Zhifeng Zhang,Xuanlan Chen

Neural regeneration research 18:2019-2028 PubMed36926728

2023

Inhibiting 5-hydroxytryptamine receptor 3 alleviates pathological changes of a mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Fen Liu,Yu-Tong Liu,Dan-Dan Wu,Jie Cheng,Na-Na Li,Ya-Ni Zheng,Liang Huang,Qiong-Lan Yuan

Cell biology international 47:480-491 PubMed36273427

2022

Inhibition of intermittent calcium-activated potassium channel (SK4) attenuates Ang II-induced hypertrophy of human-induced stem cell-derived cardiomyocytes via targeting Ras-Raf-MEK1/2-ERK1/2 and CN-NFAT signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyi Zhao,Xi Wang,Yanhong Tang,Qingyan Zhao,Congxin Huang

Clinical and experimental pharmacology & physiology 49:848-857 PubMed35596518

2022

Angiotensin receptor-neprilysin inhibitor attenuates cardiac hypertrophy and improves diastolic dysfunction in a mouse model of heart failure with preserved ejection fraction.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Zhang,Meng Yuan,Ya Suo,Qian Yang,Shuai Shao,Ying Li,Yuanyuan Wang,Qiankun Bao,Tong Liu,Guangping Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Calcineurin A antibody [EPR24997-22]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Flow Cytometry (Intracellular) - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Immunoprecipitation - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Flow Cytometry (Intracellular) - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Immunoprecipitation - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Western blot - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Western blot - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Western blot - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise