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AB154650

Anti-Calcineurin B/PPP3R1 antibody

4

(2 Reviews)

|

(6 Publications)

Rabbit Polyclonal Calcineurin B/PPP3R1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Zebrafish, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PPP3R1 aa 1-150.

View Alternative Names

CNA2, CNB, PPP3R1, Calcineurin subunit B type 1, Protein phosphatase 2B regulatory subunit 1, Protein phosphatase 3 regulatory subunit B alpha isoform 1

4 Images
Western blot - Anti-Calcineurin B/PPP3R1 antibody (AB154650)
  • WB

Supplier Data

Western blot - Anti-Calcineurin B/PPP3R1 antibody (AB154650)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-Calcineurin B/PPP3R1 antibody (ab154650) at 1/1000 dilution

All lanes:

mouse fetal brain tissue lysate at 50 µg

Predicted band size: 19 kDa

false

Western blot - Anti-Calcineurin B/PPP3R1 antibody (AB154650)
  • WB

Supplier Data

Western blot - Anti-Calcineurin B/PPP3R1 antibody (AB154650)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-Calcineurin B/PPP3R1 antibody (ab154650) at 1/100000 dilution

All lanes:

Rat brain tissue lysate at 50 µg

Predicted band size: 19 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody (AB154650)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody (AB154650)

Immunohistochemical analysis of paraffin-embedded Human NCI-N87 xenograft tissue labeling Calcineurin B/PPP3R1 with ab154650 at 1/500 dilution.

Western blot - Anti-Calcineurin B/PPP3R1 antibody (AB154650)
  • WB

Supplier Data

Western blot - Anti-Calcineurin B/PPP3R1 antibody (AB154650)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-Calcineurin B/PPP3R1 antibody (ab154650) at 1/1000 dilution

Lane 1:

Zebrafish brain tissue extracts at 30 µg

Lane 2:

Zebrafish eye tissue extracts at 30 µg

Lane 3:

Whole zebrafish tissue extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 19 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human, Zebrafish

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PPP3R1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

P63098

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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin A antibody [EPR24997-22] (AB282104)

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Primary Antibodies

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Primary Antibodies

AB24373

Anti-FKBP12 antibody

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/100000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/100000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/100000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/100000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calcineurin B also known as PPP3R1 serves as the regulatory subunit of the calcineurin phosphatase complex. The protein possesses a molecular mass of roughly 19 kDa. It expresses widely in various tissues especially in the brain skeletal muscle and immune cells. Calcineurin B partners with the catalytic subunit Calcineurin A to form the complete enzyme necessary for its dephosphorylation activity.
Biological function summary

Calcineurin B plays an important role in regulating calcium signaling pathways. It functions as part of a heterodimeric complex where it binds calcium ions and is necessary for activating the serine/threonine phosphatase activity of calcineurin. Through this activity calcineurin B influences the transcriptional activation of NFAT (Nuclear Factor of Activated T-cells) and other downstream signaling pathways affecting cell growth differentiation and immune responses.

Pathways

Calcineurin B acts critically in the calcium/calmodulin-dependent signaling pathway and the T-cell receptor signaling pathway. In these pathways it regulates the activity of proteins like NFAT and influences downstream effects essential for immune responses. The dephosphorylation activity facilitated by calcineurin B also connects it with a network of signaling cascades modulating calcium levels impacting cellular activities significantly.

Calcineurin B is associated with immunosuppressive outcomes and neurodegenerative conditions. Its connection with NFAT makes it a target for immune-related disorders such as autoimmune diseases and transplant rejection. Additionally alterations in its activity connect with proteins like tau contributing to disorders like Alzheimer's disease due to dysregulated calcium signaling and neuronal damage.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.
See full target information PPP3R1
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular biosciences 10:1292555 PubMed38094080

2023

Harnessing IGF-1 and IL-2 as biomarkers for calcineurin activity to tailor optimal FK506 dosage in α-synucleinopathies.

Applications

Unspecified application

Species

Unspecified reactive species

Sofia Zaichick,Gabriela Caraveo

PloS one 15:e0235360 PubMed32603346

2020

Sodium-calcium exchanger 1 is the key molecule for urinary potassium excretion against acute hyperkalemia.

Applications

Unspecified application

Species

Unspecified reactive species

Wakana Shoda,Naohiro Nomura,Fumiaki Ando,Hideaki Tagashira,Takahiro Iwamoto,Akihito Ohta,Kiyoshi Isobe,Takayasu Mori,Koichiro Susa,Eisei Sohara,Tatemitsu Rai,Shinichi Uchida

Journal of molecular medicine (Berlin, Germany) 96:1239-1249 PubMed30293136

2018

Nuclear calcineurin is a sensor for detecting Ca release from the nuclear envelope via IPR.

Applications

Unspecified application

Species

Unspecified reactive species

Silvana Olivares-Florez,Martin Czolbe,Fabian Riediger,Lea Seidlmayer,Tatjana Williams,Peter Nordbeck,Jörn Strasen,Cristina Glocker,Monique Jänsch,Petra Eder-Negrin,Paula Arias-Loza,Melanie Mühlfelder,Jelena Plačkić,Katrin G Heinze,Jeffery D Molkentin,Stefan Engelhardt,Jens Kockskämper,Oliver Ritter

Oncotarget 9:13407-13422 PubMed29568366

2018

Selenoprotein K deficiency inhibits melanoma by reducing calcium flux required for tumor growth and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Michael P Marciel,Vedbar S Khadka,Youping Deng,Pascal Kilicaslan,Andrew Pham,Pietro Bertino,Katie Lee,Suzie Chen,Natalija Glibetic,FuKun W Hoffmann,Michelle L Matter,Peter R Hoffmann

Science China. Life sciences 59:584-8 PubMed27053126

2016

Quality-control method for the determination of biological activity of engineered calcineurin subunit B.

Applications

Unspecified application

Species

Unspecified reactive species

Xinchang Shi,Huan Yang,Li Xu,Xiang Li,Zongwen Huang,Yudong Han,Qun Wei,Chunming Rao

Hypertension (Dallas, Tex. : 1979) 67:866-77 PubMed27021007

2016

Cardiac-Specific EPI64C Blunts Pressure Overload-Induced Cardiac Hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Xuehai Zhu,Jing Fang,Jun Gong,Jun-Hong Guo,Guang-Nian Zhao,Yan-Xiao Ji,Hong-Yun Liu,Xiang Wei,Hongliang Li
View all publications
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Product promise

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