Rabbit Polyclonal Calcineurin B/PPP3R1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PPP3R1 aa 1-150.
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Publications
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- Frontiers in molecular biosciences 10:12925552023Harnessing IGF-1 and IL-2 as biomarkers for calcineurin activity to tailor optimal FK506 dosage in α-synucleinopathies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSofia Zaichick,Gabriela CaraveoPubMed 38094080
- PloS one 15:e02353602020Sodium-calcium exchanger 1 is the key molecule for urinary potassium excretion against acute hyperkalemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWakana Shoda et. al.PubMed 32603346
- Journal of molecular medicine (Berlin, Germany) 96:1239-12492018Nuclear calcineurin is a sensor for detecting Ca release from the nuclear envelope via IPR.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSilvana Olivares-Florez et. al.PubMed 30293136
- Oncotarget 9:13407-134222018Selenoprotein K deficiency inhibits melanoma by reducing calcium flux required for tumor growth and metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael P Marciel et. al.PubMed 29568366
- Science China. Life sciences 59:584-82016Quality-control method for the determination of biological activity of engineered calcineurin subunit B.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinchang Shi et. al.PubMed 27053126
- Hypertension (Dallas, Tex. : 1979) 67:866-772016Cardiac-Specific EPI64C Blunts Pressure Overload-Induced Cardiac Hypertrophy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuehai Zhu et. al.PubMed 27021007
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PPP3R1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P63098
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Xenopus laevis, Xenopus tropicalis | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Xenopus laevis, Xenopus tropicalis | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.
Alternative names
CNA2, CNB, PPP3R1, Calcineurin subunit B type 1, Protein phosphatase 2B regulatory subunit 1, Protein phosphatase 3 regulatory subunit B alpha isoform 1
Recommended products
Rabbit Polyclonal Calcineurin B/PPP3R1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PPP3R1 aa 1-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PPP3R1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P63098
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Calcineurin B also known as PPP3R1 serves as the regulatory subunit of the calcineurin phosphatase complex. The protein possesses a molecular mass of roughly 19 kDa. It expresses widely in various tissues especially in the brain skeletal muscle and immune cells. Calcineurin B partners with the catalytic subunit Calcineurin A to form the complete enzyme necessary for its dephosphorylation activity.
- Biological function summary
Calcineurin B plays an important role in regulating calcium signaling pathways. It functions as part of a heterodimeric complex where it binds calcium ions and is necessary for activating the serine/threonine phosphatase activity of calcineurin. Through this activity calcineurin B influences the transcriptional activation of NFAT (Nuclear Factor of Activated T-cells) and other downstream signaling pathways affecting cell growth differentiation and immune responses.
- Pathways
Calcineurin B acts critically in the calcium/calmodulin-dependent signaling pathway and the T-cell receptor signaling pathway. In these pathways it regulates the activity of proteins like NFAT and influences downstream effects essential for immune responses. The dephosphorylation activity facilitated by calcineurin B also connects it with a network of signaling cascades modulating calcium levels impacting cellular activities significantly.
- Associated diseases and disorders
Calcineurin B is associated with immunosuppressive outcomes and neurodegenerative conditions. Its connection with NFAT makes it a target for immune-related disorders such as autoimmune diseases and transplant rejection. Additionally alterations in its activity connect with proteins like tau contributing to disorders like Alzheimer's disease due to dysregulated calcium signaling and neuronal damage.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Calcineurin B/PPP3R1 antibody (ab154650)
15% SDS PAGE
All lanes: Western blot - Anti-Calcineurin B/PPP3R1 antibody (ab154650) at 1/1000 dilution
Lane 1: 293T whole cell lysate at 30 µg
Lane 2: A431 whole cell lysate at 30 µg
Lane 3: H1299 whole cell lysate at 30 µg
Lane 4: HeLa whole cell lysate at 30 µg
Lane 5: Hep G2 whole cell lysate at 30 µg
Predicted band size: 19 kDa, 21 kDa, 68 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody (ab154650)
Immunohistochemical analysis of paraffin-embedded Human NCI-N87 xenograft tissue labeling Calcineurin B/PPP3R1 with ab154650 at 1/500 dilution.
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