Rabbit Polyclonal Calcineurin B/PPP3R1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human PPP3R1 aa 1 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PPP3R1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P63098
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
| Cow | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species Mouse | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.
Alternative names
CNA2, CNB, PPP3R1, Calcineurin subunit B type 1, Protein phosphatase 2B regulatory subunit 1, Protein phosphatase 3 regulatory subunit B alpha isoform 1
Recommended products
Rabbit Polyclonal Calcineurin B/PPP3R1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human PPP3R1 aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PPP3R1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P63098
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Calcineurin B also known as PPP3R1 serves as the regulatory subunit of the calcineurin phosphatase complex. The protein possesses a molecular mass of roughly 19 kDa. It expresses widely in various tissues especially in the brain skeletal muscle and immune cells. Calcineurin B partners with the catalytic subunit Calcineurin A to form the complete enzyme necessary for its dephosphorylation activity.
- Biological function summary
Calcineurin B plays an important role in regulating calcium signaling pathways. It functions as part of a heterodimeric complex where it binds calcium ions and is necessary for activating the serine/threonine phosphatase activity of calcineurin. Through this activity calcineurin B influences the transcriptional activation of NFAT (Nuclear Factor of Activated T-cells) and other downstream signaling pathways affecting cell growth differentiation and immune responses.
- Pathways
Calcineurin B acts critically in the calcium/calmodulin-dependent signaling pathway and the T-cell receptor signaling pathway. In these pathways it regulates the activity of proteins like NFAT and influences downstream effects essential for immune responses. The dephosphorylation activity facilitated by calcineurin B also connects it with a network of signaling cascades modulating calcium levels impacting cellular activities significantly.
- Associated diseases and disorders
Calcineurin B is associated with immunosuppressive outcomes and neurodegenerative conditions. Its connection with NFAT makes it a target for immune-related disorders such as autoimmune diseases and transplant rejection. Additionally alterations in its activity connect with proteins like tau contributing to disorders like Alzheimer's disease due to dysregulated calcium signaling and neuronal damage.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Western blot - Anti-Calcineurin B/PPP3R1 antibody (ab230899)
All lanes: Western blot - Anti-Calcineurin B/PPP3R1 antibody (ab230899) at 1/500 dilution
Lane 1: SH-SY5Y (human neuroblastoma cell line from bone marrow) cell extract
Lane 2: SW480 (human colorectal adenocarcinoma cell line) cell extract
Lane 3: HL-60 (human promyelocytic leukemia cell line) cell extract
Lane 4: MCF7 (human breast adenocarcinoma cell line) cell extract
Lane 5: Mouse brain cell extract
Lane 6: Mouse spleen cell extract
Lane 7: Mouse lung cell extract
Lane 8: Rat brain cell extract
Lane 9: Rat testis cell extract
Developed using the ECL technique.
Predicted band size: 19 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody (ab230899)
Formalin-fixed, paraffin-embedded human brain cortex tissue stained for Calcineurin B/PPP3R1 with ab230899 at 1/50 dilution in immunohistochemical analysis.
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