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Rabbit Recombinant Monoclonal Calcineurin B/PPP3R1 antibody. Suitable for WB, IHC-P, IP and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (AB303482), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (AB303482), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (AB303482), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (AB303482), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (AB303482), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIHC-FrFlow Cyt (Intra)IP
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Not recommended
Tested
Rat
Tested
Tested
Not recommended
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/5000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/5000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/5000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/30

Notes

-

Species

Rat

Dilution info

1/30

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

Target data

Function

Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.

Alternative names

Calcineurin subunit B type 1, Protein phosphatase 2B regulatory subunit 1, Protein phosphatase 3 regulatory subunit B alpha isoform 1, CNB, CNA2, PPP3R1

Recommended products

Rabbit Recombinant Monoclonal Calcineurin B/PPP3R1 antibody. Suitable for WB, IHC-P, IP and reacts with Human, Mouse, Rat samples.

Alternative names

Calcineurin subunit B type 1, Protein phosphatase 2B regulatory subunit 1, Protein phosphatase 3 regulatory subunit B alpha isoform 1, CNB, CNA2, PPP3R1

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR24992-18

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Calcineurin B also known as PPP3R1 serves as the regulatory subunit of the calcineurin phosphatase complex. The protein possesses a molecular mass of roughly 19 kDa. It expresses widely in various tissues especially in the brain skeletal muscle and immune cells. Calcineurin B partners with the catalytic subunit Calcineurin A to form the complete enzyme necessary for its dephosphorylation activity.

Biological function summary

Calcineurin B plays an important role in regulating calcium signaling pathways. It functions as part of a heterodimeric complex where it binds calcium ions and is necessary for activating the serine/threonine phosphatase activity of calcineurin. Through this activity calcineurin B influences the transcriptional activation of NFAT (Nuclear Factor of Activated T-cells) and other downstream signaling pathways affecting cell growth differentiation and immune responses.

Pathways

Calcineurin B acts critically in the calcium/calmodulin-dependent signaling pathway and the T-cell receptor signaling pathway. In these pathways it regulates the activity of proteins like NFAT and influences downstream effects essential for immune responses. The dephosphorylation activity facilitated by calcineurin B also connects it with a network of signaling cascades modulating calcium levels impacting cellular activities significantly.

Associated diseases and disorders

Calcineurin B is associated with immunosuppressive outcomes and neurodegenerative conditions. Its connection with NFAT makes it a target for immune-related disorders such as autoimmune diseases and transplant rejection. Additionally alterations in its activity connect with proteins like tau contributing to disorders like Alzheimer's disease due to dysregulated calcium signaling and neuronal damage.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482)

    Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling Calcineurin B/PPP3R1 with ab303482 at 1/5000 (0.097 µg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Negative control: No staining on rat cardiac muscle. The section was incubated with ab303482 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Calcineurin B/PPP3R1 with ab303482 at 1/5000 (0.097 µg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on human cerebrum. The section was incubated with ab303482 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Calcineurin B/PPP3R1 with ab303482 at 1/5000 (0.097 µg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on mouse cerebrum. The section was incubated with ab303482 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Calcineurin B/PPP3R1 with ab303482 at 1/5000 (0.097 µg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on rat cerebrum. The section was incubated with ab303482 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482)

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Calcineurin B/PPP3R1 with ab303482 at 1/5000 (0.097 µg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Negative control: No staining on human cardiac muscle. The section was incubated with ab303482 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Calcineurin B/PPP3R1 with ab303482 at 1/5000 (0.097 µg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Negative control: No staining on mouse kidney. The section was incubated with ab303482 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunoprecipitation - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482), expandable thumbnail

    Immunoprecipitation - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482)

    Calcineurin B/PPP3R1 was immunoprecipitated from 0.35 mg Rat brain tissue lysate 10 μg with ab303482 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab303482 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Rat brain tissue lysate 10 μg

    Lane 2: ab303482 IP in Rat brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab303482 in rat brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 180 seconds

    This blot was developed using a high sensitivity ECL substrate.

    All lanes: Immunoprecipitation - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482) at 1/30 dilution

    All lanes: Rat brain tissue

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 15 kDa

    Exposure time: 180s

  • Immunoprecipitation - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482), expandable thumbnail

    Immunoprecipitation - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482)

    Calcineurin B/PPP3R1 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 μg with ab303482 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab303482 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse brain tissue lysate 10 μg

    Lane 2: ab303482 IP in Mouse brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab303482 in mouse brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 180 seconds

    This blot was developed using a high sensitivity ECL substrate.

    All lanes: Immunoprecipitation - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482) at 1/30 dilution

    All lanes: Mouse brain tissue

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 15 kDa

    Exposure time: 180s

  • Western blot - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482), expandable thumbnail

    Western blot - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    This blot was developed using a high sensitivity ECL substrate.
    Exposure time: 180 seconds

    All lanes: Western blot - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482) at 1/1000 dilution

    Lane 1: SK-N-SH (human neuroblastoma epithelial cell), whole cell lysate at 20 µg

    Lane 2: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 20 µg

    Lane 3: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 15 kDa

    Exposure time: 180s

  • Western blot - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482), expandable thumbnail

    Western blot - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: heart, kidney (PMID:8110831).
    Exposure time: 10 seconds

    All lanes: Western blot - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 4: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 15 kDa

    Exposure time: 10s

  • Western blot - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482), expandable thumbnail

    Western blot - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    This antibody does not cross-react with human PPP3R2.
    Exposure time: 50 seconds

    All lanes: Western blot - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482) at 1/1000 dilution

    Lane 1: HEK-293T cells transfected with a human PPP3R1 expression vector containi a his tag, whole cell lysate at 20 µg

    Lane 2: HEK-293T cells transfected with a human PPP3R2 expression vector containing a his tag, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 20 kDa

    Exposure time: 50s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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