Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (AB303482)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Calcineurin B/PPP3R1 with ab303482 at 1/5000 (0.097 µg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Negative control : No staining on human cardiac muscle. The section was incubated with ab303482 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (AB303482)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Calcineurin B/PPP3R1 with ab303482 at 1/5000 (0.097 µg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on human cerebrum. The section was incubated with ab303482 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (AB303482)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Calcineurin B/PPP3R1 with ab303482 at 1/5000 (0.097 µg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on mouse cerebrum. The section was incubated with ab303482 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (AB303482)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Calcineurin B/PPP3R1 with ab303482 at 1/5000 (0.097 µg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on rat cerebrum. The section was incubated with ab303482 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (AB303482)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Calcineurin B/PPP3R1 with ab303482 at 1/5000 (0.097 µg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Negative control : No staining on mouse kidney. The section was incubated with ab303482 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (AB303482)

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling Calcineurin B/PPP3R1 with ab303482 at 1/5000 (0.097 µg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Negative control : No staining on rat cardiac muscle. The section was incubated with ab303482 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IP

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Immunoprecipitation - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (AB303482)

Calcineurin B/PPP3R1 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with ab303482 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab303482 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse brain tissue lysate 10 ug

Lane 2 : ab303482 IP in Mouse brain tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab303482 in mouse brain tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Immunoprecipitation - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482) at 1/1000 dilution

All lanes:

Mouse brain tissue

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 15 kDa

true

Exposure time: 180s

• IP

Supplier Data

Immunoprecipitation - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (AB303482)

Calcineurin B/PPP3R1 was immunoprecipitated from 0.35 mg Rat brain tissue lysate 10 ug with ab303482 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab303482 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Rat brain tissue lysate 10 ug

Lane 2 : ab303482 IP in Rat brain tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab303482 in rat brain tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Immunoprecipitation - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482) at 1/1000 dilution

All lanes:

Rat brain tissue

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 15 kDa

true

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (AB303482)

Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody does not cross-react with human PPP3R2. Exposure time : 50 seconds

All lanes:

Western blot - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482) at 1/1000 dilution

Lane 1:

HEK-293T cells transfected with a human PPP3R1 expression vector containi a his tag, whole cell lysate at 20 µg

Lane 2:

HEK-293T cells transfected with a human PPP3R2 expression vector containing a his tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 20 kDa

false

Exposure time: 50s

• WB

Supplier Data

Western blot - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (AB303482)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482) at 1/1000 dilution

Lane 1:

SK-N-SH (human neuroblastoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 15 kDa

true

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (AB303482)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : heart, kidney (PMID : 8110831). Exposure time : 10 seconds

All lanes:

Western blot - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse kidney tissue lysate at 20 µg

Lane 3:

Mouse heart tissue lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Lane 5:

Rat kidney tissue lysate at 20 µg

Lane 6:

Rat heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 15 kDa

false

Exposure time: 10s