AB303483

Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Calcineurin B/PPP3R1 antibody. Carrier free. Suitable for WB, IHC-P, IP and reacts with Human, Mouse, Rat samples.

View Alternative Names

CNA2, CNB, PPP3R1, Calcineurin subunit B type 1, Protein phosphatase 2B regulatory subunit 1, Protein phosphatase 3 regulatory subunit B alpha isoform 1

11 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] - BSA and Azide free (AB303483)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] - BSA and Azide free (AB303483)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] - BSA and Azide free (AB303483)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] - BSA and Azide free (AB303483)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] - BSA and Azide free (AB303483)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] - BSA and Azide free (AB303483)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] - BSA and Azide free (AB303483)

Supplier Data

Immunoprecipitation - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] - BSA and Azide free (AB303483)

This data was developed using ab303482, the same antibody clone in a different buffer formulation.

Calcineurin B/PPP3R1 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with ab303482 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab303482 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse brain tissue lysate 10 ug

Lane 2 : ab303482 IP in Mouse brain tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab303482 in mouse brain tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Immunoprecipitation - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (<a href='/en-us/products/primary-antibodies/calcineurin-b-ppp3r1-antibody-epr24992-18-ab303482'>ab303482</a>) at 1/1000 dilution

All lanes:

Mouse brain tissue

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 15 kDa

true

Exposure time: 180s

Supplier Data

Immunoprecipitation - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] - BSA and Azide free (AB303483)

This data was developed using ab303482, the same antibody clone in a different buffer formulation.

Calcineurin B/PPP3R1 was immunoprecipitated from 0.35 mg Rat brain tissue lysate 10 ug with ab303482 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab303482 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Rat brain tissue lysate 10 ug

Lane 2 : ab303482 IP in Rat brain tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab303482 in rat brain tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Rat brain tissue

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 15 kDa

true

Exposure time: 180s

Supplier Data

Western blot - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] - BSA and Azide free (AB303483)

This data was developed using ab303482, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody does not cross-react with human PPP3R2. Exposure time : 50 seconds

All lanes:

Western blot - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (<a href='/en-us/products/primary-antibodies/calcineurin-b-ppp3r1-antibody-epr24992-18-ab303482'>ab303482</a>) at 1/1000 dilution

Lane 1:

HEK-293T cells transfected with a human PPP3R1 expression vector containi a his tag, whole cell lysate at 20 µg

Lane 2:

HEK-293T cells transfected with a human PPP3R2 expression vector containing a his tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 20 kDa

false

Exposure time: 50s

Supplier Data

Western blot - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] - BSA and Azide free (AB303483)

This data was developed using ab303482, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (<a href='/en-us/products/primary-antibodies/calcineurin-b-ppp3r1-antibody-epr24992-18-ab303482'>ab303482</a>) at 1/1000 dilution

Lane 1:

SK-N-SH (human neuroblastoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 15 kDa

true

Exposure time: 180s

Supplier Data

Western blot - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] - BSA and Azide free (AB303483)

This data was developed using ab303482, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : heart, kidney (PMID : 8110831). Exposure time : 10 seconds

All lanes:

Western blot - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (<a href='/en-us/products/primary-antibodies/calcineurin-b-ppp3r1-antibody-epr24992-18-ab303482'>ab303482</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse kidney tissue lysate at 20 µg

Lane 3:

Mouse heart tissue lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Lane 5:

Rat kidney tissue lysate at 20 µg

Lane 6:

Rat heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 15 kDa

false

Exposure time: 10s

Unconjugated

Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24992-18

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "The antibody exhibits low sensitivity when detecting in cell line assays. It is recommended to optimize experimental conditions by increasing the sample loading amount, using a lower antibody dilution ratio, and employing femtogram-level sensitivity substrates.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "The antibody exhibits low sensitivity when detecting in cell line assays. It is recommended to optimize experimental conditions by increasing the sample loading amount, using a lower antibody dilution ratio, and employing femtogram-level sensitivity substrates.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "The antibody exhibits low sensitivity when detecting in cell line assays. It is recommended to optimize experimental conditions by increasing the sample loading amount, using a lower antibody dilution ratio, and employing femtogram-level sensitivity substrates.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calcineurin B also known as PPP3R1 serves as the regulatory subunit of the calcineurin phosphatase complex. The protein possesses a molecular mass of roughly 19 kDa. It expresses widely in various tissues especially in the brain skeletal muscle and immune cells. Calcineurin B partners with the catalytic subunit Calcineurin A to form the complete enzyme necessary for its dephosphorylation activity.

Biological function summary

Calcineurin B plays an important role in regulating calcium signaling pathways. It functions as part of a heterodimeric complex where it binds calcium ions and is necessary for activating the serine/threonine phosphatase activity of calcineurin. Through this activity calcineurin B influences the transcriptional activation of NFAT (Nuclear Factor of Activated T-cells) and other downstream signaling pathways affecting cell growth differentiation and immune responses.

Pathways

Calcineurin B acts critically in the calcium/calmodulin-dependent signaling pathway and the T-cell receptor signaling pathway. In these pathways it regulates the activity of proteins like NFAT and influences downstream effects essential for immune responses. The dephosphorylation activity facilitated by calcineurin B also connects it with a network of signaling cascades modulating calcium levels impacting cellular activities significantly.

Calcineurin B is associated with immunosuppressive outcomes and neurodegenerative conditions. Its connection with NFAT makes it a target for immune-related disorders such as autoimmune diseases and transplant rejection. Additionally alterations in its activity connect with proteins like tau contributing to disorders like Alzheimer's disease due to dysregulated calcium signaling and neuronal damage.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.

See full target information PPP3R1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] - BSA and Azide free (AB303483)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] - BSA and Azide free (AB303483)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] - BSA and Azide free (AB303483)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] - BSA and Azide free (AB303483)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] - BSA and Azide free (AB303483)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] - BSA and Azide free (AB303483)

Immunoprecipitation - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] - BSA and Azide free (AB303483)

Immunoprecipitation - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] - BSA and Azide free (AB303483)

Western blot - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] - BSA and Azide free (AB303483)

Western blot - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] - BSA and Azide free (AB303483)

Western blot - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] - BSA and Azide free (AB303483)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise