Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] (ab303562)

Rabbit Recombinant Monoclonal Calcineurin B/PPP3R1 antibody. Suitable for WB, IHC-P, IP and reacts with Human, Mouse, Rat samples.

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Images

Immunoprecipitation - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] (AB303562), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	ICC/IF	Flow Cyt (Intra)	IP
Human	Tested	Tested	Not recommended	Not recommended	Tested
Mouse	Tested	Tested	Not recommended	Not recommended	Not recommended
Rat	Tested	Tested	Not recommended	Not recommended	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/10000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/10000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/10000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Rat, Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info 1/30	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Target data

See full target information Calcineurin B/PPP3R1

Function

Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.

Additional Targets

PPP3R2, PPP3R2

Alternative names

CNA2, CNB, PPP3R1, Calcineurin subunit B type 1, Protein phosphatase 2B regulatory subunit 1, Protein phosphatase 3 regulatory subunit B alpha isoform 1

Recommended products

Rabbit Recombinant Monoclonal Calcineurin B/PPP3R1 antibody. Suitable for WB, IHC-P, IP and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR24992-14
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Calcineurin B is a regulatory subunit of the enzyme calcineurin known alternatively as PPP3R1 for Calcineurin BI and PPP3R2 for Calcineurin BII. This protein weighs approximately 19 kDa. Calcineurin B is expressed in various tissues including brain muscle and immune cells. Its primary function is to bind calcium ions facilitating conformational changes that enable the activation of the catalytic subunit of calcineurin. Through these changes it maintains a connection between calcium signaling and other cellular processes.

Biological function summary

Calcineurin B plays an essential role in intracellular signaling by being part of the calcineurin complex which also includes the catalytic subunit calcineurin A. This complex is vital for the dephosphorylation of the nuclear factor of activated T-cells (NFAT) which then translocates to the nucleus to influence the expression of various genes. By controlling gene transcription calcineurin B contributes significantly to immune response muscle development and brain function.

Pathways

Calcineurin B facilitates various cellular reactions as an integral component of calcium signaling and immune response. It is significantly involved in the T-cell receptor signaling pathway where its activity helps regulate T-cell activation and differentiation. Calcineurin B also plays a role in the synaptic plasticity pathway important for learning and memory functions in the brain. It interacts closely with proteins like NFATc and calmodulin that further extend its influence across other signaling pathways.

Associated diseases and disorders

Calcineurin B is implicated in conditions such as autoimmune diseases and cardiac hypertrophy. In autoimmune diseases disrupted calcineurin function can lead to improper T-cell activity contributing to diseases like rheumatoid arthritis. In cardiac hypertrophy altered calcineurin signaling can affect heart muscle growth and function. Calcineurin B is connected to the protein FKBP12 which is part of the immunosuppressive drug complex influencing its role in these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Immunoprecipitation - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] (ab303562)
Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 was immunoprecipitated from 0.35 mg HEL (human erythroleukemia erythroblast), whole cell lysate with ab303562 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab303562 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HEL (human erythroleukemia erythroblast), whole cell lysate (Inset)
Lane 2: ab303562 IP in HEL whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab303562 in HEL whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
Lanes 1 - 2: Immunoprecipitation - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] (ab303562) at 1/1000 dilution
Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)
Lane 1: HEL (human erythroleukemia erythroblast), whole cell lysate (Inset)
Lane 2: ab303562 IP in HEL whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab303562 in HEL whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Developed using the ECL technique.
Exposure time: 110s
Western blot - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] (ab303562)
Blocking / Diluting buffer and concentration: 5% NFDM/TBST
The identity of the higher MW band at approximately 75kDa is unknown.
All lanes: Western blot - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] (ab303562) at 1/1000 dilution
Lane 1: HEK-293T cells transfected with a human Calcineurin B/PPP3R1 expression vector containing a his tag, whole cell lysate at 1 µg
Lane 2: HEK-293T cells transfected with a human Calcineurin BII/PPP3R2 expression vector containing a his tag, whole cell lysate at 75 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 20 kDa
Exposure time: 7.75s
Western blot - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] (ab303562)
Blocking / Diluting buffer and concentration: 5% NFDM/TBST
Low expression: heart, kidney (PMID:8110831).
The identity of the higher MW band at approximately 75 kDa is unknown.
All lanes: Western blot - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] (ab303562) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 40 µg
Lane 2: Mouse kidney tissue lysate at 40 µg
Lane 3: Mouse heart tissue lysate at 40 µg
Lane 4: Rat brain tissue lysate at 40 µg
Lane 5: Rat kidney tissue lysate at 40 µg
Lane 6: Rat heart tissue lysate at 40 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 15 kDa
Exposure time: 10s
Western blot - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] (ab303562)
Blocking / Diluting buffer and concentration: 5% NFDM/TBST
The higher MW band at approximately 18 kDa (in lane 2) is PPP3R2. (PMID: 26429887)
All lanes: Western blot - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] (ab303562) at 1/1000 dilution
Lane 1: Human testis tissue lysate at 20 µg
Lane 2: Mouse testis tissue lysate at 20 µg
Lane 3: Rat testis tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 15,18 kDa
Exposure time: 136s
Western blot - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] (ab303562)
Blocking / Diluting buffer and concentration: 5% NFDM/TBST
Low expression: heart, kidney (PMID:8110831).
The molecular weight observed is consistent with what has been described in the literature (PMID: 30262862).
The identity of the higher MW band at approximately 75 kDa (in lane 3) is unknown.
All lanes: Western blot - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] (ab303562) at 1/1000 dilution
Lane 1: Human cerebellum tissue lysate at 40 µg
Lane 2: Human kidney tissue lysate at 40 µg
Lane 3: Human heart tissue lysate at 40 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 15 kDa
Western blot - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] (ab303562)
Blocking / Diluting buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] (ab303562) at 1/1000 dilution
Lane 1: HEL (human erythroleukemia erythroblast), whole cell lysate at 40 µg
Lane 2: C6 (rat glial tumor glial cell), whole cell lysate at 40 µg
Lane 3: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 40 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Exposure time: 70s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] (ab303562)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 with ab303562 at 1/10000 (0.052 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Negative control: No staining on human kidney. The section was incubated with ab303562 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] (ab303562)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 with ab303562 at 1/10000 (0.052 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on rat cerebrum. The section was incubated with ab303562 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] (ab303562)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 with ab303562 at 1/10000 (0.052 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on mouse cerebrum. The section was incubated with ab303562 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] (ab303562)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 with ab303562 at 1/10000 (0.052 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on human cerebrum. The section was incubated with ab303562 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins