AB303563

Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Calcineurin B/PPP3R1 antibody. Carrier free. Suitable for WB, IHC-P, IP and reacts with Human, Mouse, Rat samples.

View Alternative Names

CNA2, CNB, PPP3R1, Calcineurin subunit B type 1, Protein phosphatase 2B regulatory subunit 1, Protein phosphatase 3 regulatory subunit B alpha isoform 1

10 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] - BSA and Azide free (AB303563)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] - BSA and Azide free (AB303563)

This data was developed using ab303562, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 with ab303562 at 1/10000 (0.052 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on human cerebrum. The section was incubated with ab303562 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] - BSA and Azide free (AB303563)

This data was developed using ab303562, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 with ab303562 at 1/10000 (0.052 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Negative control : No staining on human kidney. The section was incubated with ab303562 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Supplier Data

Immunoprecipitation - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] - BSA and Azide free (AB303563)

This data was developed using ab303562, the same antibody clone in a different buffer formulation.

Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 was immunoprecipitated from 0.35 mg HEL (human erythroleukemia erythroblast), whole cell lysate with ab303562 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab303562 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : HEL (human erythroleukemia erythroblast), whole cell lysate (Inset)

Lane 2 : ab303562 IP in HEL whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab303562 in HEL whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Immunoprecipitation - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] (<a href='/en-us/products/primary-antibodies/calcineurin-b-ppp3r1-calcineurin-bii-ppp3r2-antibody-epr24992-14-ab303562'>ab303562</a>) at 1/1000 dilution

Lane 1:

HEL (human erythroleukemia erythroblast), whole cell lysate (Inset)

Lane 2:

<a href='/en-us/products/primary-antibodies/calcineurin-b-ppp3r1-calcineurin-bii-ppp3r2-antibody-epr24992-14-ab303562'>ab303562</a> IP in HEL whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/en-us/products/primary-antibodies/calcineurin-b-ppp3r1-calcineurin-bii-ppp3r2-antibody-epr24992-14-ab303562'>ab303562</a> in HEL whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

true

Exposure time: 110s

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] - BSA and Azide free (AB303563)

This data was developed using ab303562, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 with ab303562 at 1/10000 (0.052 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on rat cerebrum. The section was incubated with ab303562 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] - BSA and Azide free (AB303563)

This data was developed using ab303562, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 with ab303562 at 1/10000 (0.052 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on mouse cerebrum. The section was incubated with ab303562 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Supplier Data

Western blot - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] - BSA and Azide free (AB303563)

This data was developed using ab303562, the same antibody clone in a different buffer formulation.

Blocking / Diluting buffer and concentration : 5% NFDM/TBST

Low expression : heart, kidney (PMID : 8110831).

The molecular weight observed is consistent with what has been described in the literature (PMID : 30262862).

The identity of the higher MW band at approximately 75 kDa (in lane 3) is unknown.

All lanes:

Western blot - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] (<a href='/en-us/products/primary-antibodies/calcineurin-b-ppp3r1-calcineurin-bii-ppp3r2-antibody-epr24992-14-ab303562'>ab303562</a>) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 40 µg

Lane 2:

Human kidney tissue lysate at 40 µg

Lane 3:

Human heart tissue lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 15 kDa

false

Supplier Data

Western blot - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] - BSA and Azide free (AB303563)

This data was developed using ab303562, the same antibody clone in a different buffer formulation.

Blocking / Diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Lane 1:

HEL (human erythroleukemia erythroblast), whole cell lysate at 40 µg

Lane 2:

C6 (rat glial tumor glial cell), whole cell lysate at 40 µg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 70s

Supplier Data

Western blot - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] - BSA and Azide free (AB303563)

This data was developed using ab303562, the same antibody clone in a different buffer formulation.

Blocking / Diluting buffer and concentration : 5% NFDM/TBST

The higher MW band at approximately 18 kDa (in lane 2) is PPP3R2. (PMID : 26429887)

All lanes:

Lane 1:

Human testis tissue lysate at 20 µg

Lane 2:

Mouse testis tissue lysate at 20 µg

Lane 3:

Rat testis tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 15 kDa,18 kDa

false

Exposure time: 136s

Supplier Data

Western blot - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] - BSA and Azide free (AB303563)

This data was developed using ab303562, the same antibody clone in a different buffer formulation.

Blocking / Diluting buffer and concentration : 5% NFDM/TBST

Low expression : heart, kidney (PMID : 8110831).

The identity of the higher MW band at approximately 75 kDa is unknown.

All lanes:

Lane 1:

Mouse brain tissue lysate at 40 µg

Lane 2:

Mouse kidney tissue lysate at 40 µg

Lane 3:

Mouse heart tissue lysate at 40 µg

Lane 4:

Rat brain tissue lysate at 40 µg

Lane 5:

Rat kidney tissue lysate at 40 µg

Lane 6:

Rat heart tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 15 kDa

false

Exposure time: 10s

Supplier Data

Western blot - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] - BSA and Azide free (AB303563)

This data was developed using ab303562, the same antibody clone in a different buffer formulation.

Blocking / Diluting buffer and concentration : 5% NFDM/TBST

The identity of the higher MW band at approximately 75kDa is unknown.

All lanes:

Lane 1:

HEK-293T cells transfected with a human Calcineurin B/PPP3R1 expression vector containing a his tag, whole cell lysate at 1 µg

Lane 2:

HEK-293T cells transfected with a human Calcineurin BII/PPP3R2 expression vector containing a his tag, whole cell lysate at 75 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 20 kDa

false

Exposure time: 7.75s

Unconjugated

Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14]
Carrier free

Anti-Calcineurin B/PPP3R1+Calcineurin BII/PPP3R2 antibody [EPR24992-14] -BSA & Azide free (Detector)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24992-14

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calcineurin B is a regulatory subunit of the enzyme calcineurin known alternatively as PPP3R1 for Calcineurin BI and PPP3R2 for Calcineurin BII. This protein weighs approximately 19 kDa. Calcineurin B is expressed in various tissues including brain muscle and immune cells. Its primary function is to bind calcium ions facilitating conformational changes that enable the activation of the catalytic subunit of calcineurin. Through these changes it maintains a connection between calcium signaling and other cellular processes.

Biological function summary

Calcineurin B plays an essential role in intracellular signaling by being part of the calcineurin complex which also includes the catalytic subunit calcineurin A. This complex is vital for the dephosphorylation of the nuclear factor of activated T-cells (NFAT) which then translocates to the nucleus to influence the expression of various genes. By controlling gene transcription calcineurin B contributes significantly to immune response muscle development and brain function.

Pathways

Calcineurin B facilitates various cellular reactions as an integral component of calcium signaling and immune response. It is significantly involved in the T-cell receptor signaling pathway where its activity helps regulate T-cell activation and differentiation. Calcineurin B also plays a role in the synaptic plasticity pathway important for learning and memory functions in the brain. It interacts closely with proteins like NFATc and calmodulin that further extend its influence across other signaling pathways.

Calcineurin B is implicated in conditions such as autoimmune diseases and cardiac hypertrophy. In autoimmune diseases disrupted calcineurin function can lead to improper T-cell activity contributing to diseases like rheumatoid arthritis. In cardiac hypertrophy altered calcineurin signaling can affect heart muscle growth and function. Calcineurin B is connected to the protein FKBP12 which is part of the immunosuppressive drug complex influencing its role in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.

See full target information PPP3R1

Additional targets

PPP3R2,PPP3R2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] - BSA and Azide free (AB303563)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] - BSA and Azide free (AB303563)

Immunoprecipitation - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] - BSA and Azide free (AB303563)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] - BSA and Azide free (AB303563)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] - BSA and Azide free (AB303563)

Western blot - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] - BSA and Azide free (AB303563)

Western blot - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] - BSA and Azide free (AB303563)

Western blot - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] - BSA and Azide free (AB303563)

Western blot - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] - BSA and Azide free (AB303563)

Western blot - Anti-Calcineurin B/PPP3R1 + Calcineurin BII/PPP3R2 antibody [EPR24992-14] - BSA and Azide free (AB303563)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise